BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_I16 (600 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 27 2.8 SPBC354.03 |swd3||WD repeat protein Swd3|Schizosaccharomyces pom... 27 2.8 SPAC1565.01 |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.4 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 6.4 SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 8.5 >SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosaccharomyces pombe|chr 1|||Manual Length = 180 Score = 26.6 bits (56), Expect = 2.8 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = -2 Query: 509 VLEHVVHSGTDSVEVRNLRNIWQVFSGECF-GTEIVVVVMEEIYFTGSRH 363 +L HV + GT+SV++ R I Q F +CF V + E +F RH Sbjct: 111 ILFHVTYDGTESVDL--ARPIIQEFFLKCFLQNNKSVTPVYESFFNQPRH 158 >SPBC354.03 |swd3||WD repeat protein Swd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 380 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Frame = -2 Query: 458 LRNIWQVFSGECFGT--EIVVVVMEEIYFTGSRHFV 357 + IW V SG+C T E + V + + FT +R ++ Sbjct: 203 MARIWDVLSGQCLKTLVEPINVPLSNLQFTENRKYL 238 >SPAC1565.01 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 25.4 bits (53), Expect = 6.4 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = +3 Query: 480 GAGVDYMFKDKIGASATAAHTDVFNRNDYSLGG 578 G+G +F DK G A F++ DYS G Sbjct: 61 GSGASIIFADKAGLKFEAERYGKFDQIDYSTKG 93 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 25.4 bits (53), Expect = 6.4 Identities = 20/88 (22%), Positives = 40/88 (45%), Gaps = 3/88 (3%) Frame = +3 Query: 129 SRVRRQAGALTVNSDGTSGAMVKVPITGNENHRLSALGSVDLTNQMKLGAATAGLAYD-- 302 S + +++G + + +GT G + G + S +++ + + + AA + + Sbjct: 1228 SVINQESGKIELFMEGT-GLQAVMNTEGIVGTKTSTNHVMEMKDVLGIEAARYSIISEIG 1286 Query: 303 -NVNGHGATLTKTHIPGFGDKMTAAGKV 383 + HG T+ HI GD MT G+V Sbjct: 1287 YTMAKHGLTVDPRHIMLLGDVMTCKGEV 1314 >SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 25.0 bits (52), Expect = 8.5 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +3 Query: 300 DNVNGHGATLTKTHIPGFGDKMTAAGKVNL 389 +N AT T T GFGDK + AGK N+ Sbjct: 251 ENKESTTATSTLTDA-GFGDKSSTAGKHNV 279 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,617,133 Number of Sequences: 5004 Number of extensions: 54262 Number of successful extensions: 142 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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