BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_I15 (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 155 4e-39 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 150 2e-37 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 28 1.2 SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 1.6 SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 1.6 SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 27 2.9 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 27 2.9 SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 26 3.8 SPAC22F8.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.8 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 8.8 SPBC947.11c |elg1||DNA replication factor C complex subunit Elg1... 25 8.8 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 155 bits (377), Expect = 4e-39 Identities = 79/122 (64%), Positives = 93/122 (76%), Gaps = 1/122 (0%) Frame = -3 Query: 465 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 286 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK EIREKLA+MYK + V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59 Query: 285 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 109 +T+FGGG+STGFALIYD+ + KKFEP +RL R G E ++ R+QRK+RKNR KKV Sbjct: 60 LRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVF 119 Query: 108 GT 103 GT Sbjct: 120 GT 121 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 150 bits (363), Expect = 2e-37 Identities = 74/122 (60%), Positives = 91/122 (74%), Gaps = 1/122 (0%) Frame = -3 Query: 465 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 286 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK ++REKL +MYK V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59 Query: 285 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 109 +T++GGG++TGFALIYD ++ KKFEP +RL R G E ++ R+QRK+RKNR KKV Sbjct: 60 LRTHYGGGRTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVF 119 Query: 108 GT 103 GT Sbjct: 120 GT 121 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -3 Query: 213 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 115 K+ P R G + +KR RK++ E+KN K Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571 >SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 27.5 bits (58), Expect = 1.6 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 348 EIREKLAKMYKVTPDVVFVFGFKTNFGG 265 +++E LAK YK+ P +F F T+ G Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313 >SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 27.5 bits (58), Expect = 1.6 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +1 Query: 277 RLETEHENY-VWRHFVHFSELFTDLSFADGRFSWMQDIAYHL 399 RLE EH V R+ L +F D R+ W Q+I+ L Sbjct: 261 RLEVEHPGEEVLRNNRILGRLMPSRAFGDARYKWSQEISERL 302 >SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharomyces pombe|chr 1|||Manual Length = 671 Score = 26.6 bits (56), Expect = 2.9 Identities = 16/62 (25%), Positives = 26/62 (41%), Gaps = 1/62 (1%) Frame = -3 Query: 519 PRAEFGTRFTPKSRK*SKMSEGTATIRTRKFMTNRLLARKQMVC-DVLHPGKPTVSKTEI 343 P+ ++ TR T +S S + KF+ L A K +C D++ +T I Sbjct: 102 PKDDYKTRITLRSPSGSYRGSVVCLFKRSKFLPEELPADKSQICTDIIDDASGCAWETRI 161 Query: 342 RE 337 E Sbjct: 162 DE 163 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 26.6 bits (56), Expect = 2.9 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = -1 Query: 173 MKRRGRHANSVRNVRTE*RRYEVPRN 96 +KRR R N++R + E RRY +P++ Sbjct: 28 VKRRKRLFNAIRKLWNERRRYRMPKD 53 >SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 26.2 bits (55), Expect = 3.8 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = +3 Query: 105 YLVPSSFCSYVPYAVCVSASSFHTGRDERVCVLAQTSL 218 Y VP + A+ V A H G+D+ C LA+ L Sbjct: 65 YDVPFLINDRIDVALAVGADGVHIGQDDMDCALARKIL 102 >SPAC22F8.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 133 Score = 25.0 bits (52), Expect = 8.8 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +1 Query: 100 LGTSYLLHSVLTFLTLFACRPLLFIQAVTSESVFWL 207 L TS+ S+L L + PLLF+ + S S + L Sbjct: 17 LNTSFSFSSLLCILCFYKDGPLLFLYSPLSASYYCL 52 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 25.0 bits (52), Expect = 8.8 Identities = 13/46 (28%), Positives = 20/46 (43%) Frame = +1 Query: 250 SGRFATTEVRLETEHENYVWRHFVHFSELFTDLSFADGRFSWMQDI 387 +G +A E HENY H E++ D + R ++ DI Sbjct: 60 NGMYAGAENHNVENHENYTMVGHDHMEEVYGDDLVNEPRIAYSSDI 105 >SPBC947.11c |elg1||DNA replication factor C complex subunit Elg1|Schizosaccharomyces pombe|chr 2|||Manual Length = 920 Score = 25.0 bits (52), Expect = 8.8 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +2 Query: 191 SLCFGSNFFAKSSVS*IKANPVDLP 265 S CF +N F+KSS+ I +N +P Sbjct: 766 SFCFINNLFSKSSLKAISSNDSIVP 790 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,405,123 Number of Sequences: 5004 Number of extensions: 50003 Number of successful extensions: 149 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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