BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_H23 (556 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 224 8e-60 SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 196 2e-51 SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 99 4e-22 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.021 SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.35 SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 29 0.35 SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 28 0.80 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.1 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.4 SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.9 SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 26 4.3 SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|... 26 4.3 SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pomb... 25 5.7 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 25 5.7 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 5.7 SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 25 7.5 SPAC23C4.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 9.9 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 25 9.9 SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 25 9.9 SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual 25 9.9 SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 9.9 >SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 224 bits (547), Expect = 8e-60 Identities = 111/147 (75%), Positives = 128/147 (87%) Frame = +2 Query: 110 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 289 P+Y PFFGVMG +AI+F+S GAAYGTAK+G GI+AM V+RP+ I+K+ IPVVMAGIIAI Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66 Query: 290 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 469 YGLVV+VLI+G+L Q +LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQ Sbjct: 67 YGLVVSVLISGNLKQI---LSLYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQ 123 Query: 470 PRLFVGMILILIFAEVXGLYGLIVAIL 550 PRLFV MILILIFAEV GLYGLIVA+L Sbjct: 124 PRLFVAMILILIFAEVLGLYGLIVALL 150 >SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 196 bits (477), Expect = 2e-51 Identities = 91/147 (61%), Positives = 117/147 (79%) Frame = +2 Query: 110 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 289 PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE +MKS+IPVVM+GII + Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66 Query: 290 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 469 YGLV++VLIAG + P N+Y+L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q Sbjct: 67 YGLVMSVLIAGDMS-PDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQ 125 Query: 470 PRLFVGMILILIFAEVXGLYGLIVAIL 550 R+FV M+LILIFAEV GLYGLIV ++ Sbjct: 126 DRIFVSMVLILIFAEVLGLYGLIVGLI 152 >SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 99.1 bits (236), Expect = 4e-22 Identities = 51/146 (34%), Positives = 80/146 (54%), Gaps = 5/146 (3%) Frame = +2 Query: 128 FGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIYGLVVA 307 +G++G AS + F +GAA+G GT I AV P K++I ++ ++AIY L++A Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104 Query: 308 VLIAGSLDQ--PSNNYT---LYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 472 ++ + ++ P+ YT Y GF G+ VG L G +GI G + AQ Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164 Query: 473 RLFVGMILILIFAEVXGLYGLIVAIL 550 LFV ++++ IF V GL+GLIV +L Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLL 190 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 33.5 bits (73), Expect = 0.021 Identities = 20/57 (35%), Positives = 28/57 (49%) Frame = -2 Query: 531 PYRPXTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTARPAPK*MNP 361 P RP ++A ++ PT +VP P++P MP P+A P A AP NP Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 29.5 bits (63), Expect = 0.35 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = -2 Query: 534 SPYRPXTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTAR 385 S Y+P +S+K+ + + G A+P+ P P P +P R T + Sbjct: 401 SSYKPSSSSKVSAKSVSRKPTGAPAIPKLP--PKHPSRQPTVRATPSTGK 448 >SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 29.5 bits (63), Expect = 0.35 Identities = 21/69 (30%), Positives = 30/69 (43%) Frame = +2 Query: 254 IIPVVMAGIIAIYGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGI 433 II +AG+IA + +++ IAG + G ++ G L LA GF I Sbjct: 193 IITATIAGVIAAFSVIITATIAGVI-------AAMVGILYFGHWLVYKILILAFGFKIVT 245 Query: 434 VGDAGVRGT 460 GD V T Sbjct: 246 SGDVCVSNT 254 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 28.3 bits (60), Expect = 0.80 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -3 Query: 113 LDFRPFFLNVGVFTGPKNSDDYLHTL 36 +DF P V TGP N+DDY+H + Sbjct: 357 MDF-PNITQVVQITGPSNTDDYIHRI 381 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.1 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 2/31 (6%) Frame = -2 Query: 462 AVPRTPAS--PTMPMAKPAARPEKPTARPAP 376 A P P S P++PM PAA P P+A AP Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 27.5 bits (58), Expect = 1.4 Identities = 16/80 (20%), Positives = 37/80 (46%), Gaps = 2/80 (2%) Frame = +2 Query: 125 FFG-VMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMR-PEQIMKSIIPVVMAGIIAIYGL 298 F+G ++G + F+ + + ++ G + I+ S+ P+V++ IIA + Sbjct: 1439 FYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFF 1498 Query: 299 VVAVLIAGSLDQPSNNYTLY 358 + +++ L S Y +Y Sbjct: 1499 CLGIMLRPILGDRSKTYGVY 1518 >SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 27.1 bits (57), Expect = 1.9 Identities = 21/72 (29%), Positives = 33/72 (45%) Frame = +2 Query: 113 IYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIY 292 +YGPF+ A+ FS+ Y +G G + ++ K +I A II Y Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137 Query: 293 GLVVAVLIAGSL 328 ++AVL+ G L Sbjct: 138 TTIIAVLLWGIL 149 >SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 579 Score = 25.8 bits (54), Expect = 4.3 Identities = 13/50 (26%), Positives = 26/50 (52%), Gaps = 2/50 (4%) Frame = +2 Query: 296 LVVAVLIAGSLDQPSNNYTLYK--GFIHLGAGLAVGFSGLAAGFAIGIVG 439 L+ + ++ + +N +Y+ G + +A+G +GLA G IG+ G Sbjct: 141 LITSSMLLDTFQTMESNQEMYELSGTRKMRRRIAMGLAGLAGGALIGLTG 190 >SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 397 Score = 25.8 bits (54), Expect = 4.3 Identities = 12/29 (41%), Positives = 15/29 (51%) Frame = +3 Query: 21 VVSWSECVQIVIRVFGTCKYSHIQEKWPK 107 V S V I + F + SH+QEKW K Sbjct: 96 VFSLIAVVLITVMGFAMLRVSHLQEKWRK 124 >SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 287 Score = 25.4 bits (53), Expect = 5.7 Identities = 13/45 (28%), Positives = 21/45 (46%) Frame = +3 Query: 84 HIQEKWPKIQSMDLSLELWGRRLPSSSAPLVLHMAQPSQAPVLPP 218 HI WP+ QS D E G L + A + + + ++ +L P Sbjct: 62 HIATLWPETQSSDQENEEEGEELEDAVAKEINSLQKKNKKELLTP 106 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 25.4 bits (53), Expect = 5.7 Identities = 19/61 (31%), Positives = 24/61 (39%), Gaps = 10/61 (16%) Frame = -2 Query: 534 SPYRPXTSAKMRIRIIPTNNLGCWAVPRT----------PASPTMPMAKPAARPEKPTAR 385 S RP TSA R I P + +P PA P P+ P + P P+A Sbjct: 135 SELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAV 194 Query: 384 P 382 P Sbjct: 195 P 195 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 5.7 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = -2 Query: 462 AVPRTPASPTMPMA----KPAARPEKPTARPAPK 373 +VP+ PA+P +P A +P A P P AP+ Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546 >SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizosaccharomyces pombe|chr 3|||Manual Length = 920 Score = 25.0 bits (52), Expect = 7.5 Identities = 12/40 (30%), Positives = 19/40 (47%) Frame = -3 Query: 125 KVHRLDFRPFFLNVGVFTGPKNSDDYLHTLTPRNYFSTDK 6 KV +D + FL PKN +DY++ L + D+ Sbjct: 249 KVQNVDQKSGFLTSSETDVPKNINDYIYLLIDDRFVPPDR 288 >SPAC23C4.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 104 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/42 (26%), Positives = 23/42 (54%) Frame = +1 Query: 76 NTPTFKKNGRKSNLWTFLWSYGGGVCHHLQLPWCCIWHSQVR 201 ++PT + + W S+G VC H++ CC ++S+++ Sbjct: 32 DSPTTVHHDERKESWR---SHGFIVCLHIRCCICCKYNSKIK 70 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 24.6 bits (51), Expect = 9.9 Identities = 8/31 (25%), Positives = 19/31 (61%) Frame = -2 Query: 255 MDFMICSGRITAMAAIPVPDLAVPYAAPREL 163 ++ ++ +G ++ +P+ DLA Y A R++ Sbjct: 283 LEAIVATGAKVVLSKLPIGDLATQYFADRDI 313 >SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 708 Score = 24.6 bits (51), Expect = 9.9 Identities = 12/32 (37%), Positives = 15/32 (46%) Frame = -2 Query: 480 NNLGCWAVPRTPASPTMPMAKPAARPEKPTAR 385 +N+ V P +P M M PA P K AR Sbjct: 256 SNMHIRYVSANPKTPVMYMVDPAYEPHKQIAR 287 >SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 478 Score = 24.6 bits (51), Expect = 9.9 Identities = 7/16 (43%), Positives = 10/16 (62%) Frame = -3 Query: 236 QAASPPWRQYRCLTWL 189 Q ++PPW C +WL Sbjct: 448 QGSAPPWHYIPCQSWL 463 >SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = +1 Query: 52 SSEFLGPVNTPTFKKNG 102 SS +L P +P FKKNG Sbjct: 169 SSGYLHPKTSPIFKKNG 185 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,627,563 Number of Sequences: 5004 Number of extensions: 60719 Number of successful extensions: 188 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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