Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_G20
(608 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g54030.1 68418.m06720 DC1 domain-containing protein contains ... 33 0.15
At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type f... 32 0.34
At5g60920.1 68418.m07642 phytochelatin synthetase, putative / CO... 31 0.60
At2g17620.1 68415.m02038 cyclin, putative (CYC2a) similar to cyc... 31 0.60
At5g05660.1 68418.m00622 zinc finger (NF-X1 type) family protein... 30 1.4
At3g17750.1 68416.m02265 protein kinase family protein contains ... 30 1.4
At5g55960.1 68418.m06979 expressed protein 29 1.8
At3g62810.1 68416.m07057 complex 1 family protein / LVR family p... 29 3.2
At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type f... 29 3.2
At1g73460.1 68414.m08504 protein kinase family protein contains ... 29 3.2
At1g73450.1 68414.m08503 protein kinase, putative similar to nuc... 29 3.2
At5g56050.1 68418.m06993 hypothetical protein 28 5.6
At1g61870.1 68414.m06981 pentatricopeptide (PPR) repeat-containi... 28 5.6
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 27 7.4
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 27 7.4
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 27 7.4
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 27 7.4
At5g42620.1 68418.m05188 expressed protein 27 9.7
>At5g54030.1 68418.m06720 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 419
Score = 33.1 bits (72), Expect = 0.15
Identities = 18/68 (26%), Positives = 30/68 (44%)
Frame = +2
Query: 125 KIYSPVCGSDGKTYENPCEFYCEKDKTHSNMTIVKNTACEVGIPCYCTLEYAPVCGSNGK 304
K+Y+ + S G+ YEN C F C+ + + T C + + C P+ S K
Sbjct: 83 KVYTHIFTSSGRIYENTCHF-CQ---SKLEFLFARCTICNLNVDIECLFALPPLTISEPK 138
Query: 305 TYANKCSL 328
+ + SL
Sbjct: 139 HHKHSLSL 146
>At4g01575.1 68417.m00205 serine protease inhibitor, Kazal-type
family protein contains Pfam domain PF00050: Kazal-type
serine protease inhibitor domain
Length = 144
Score = 31.9 bits (69), Expect = 0.34
Identities = 25/69 (36%), Positives = 32/69 (46%), Gaps = 4/69 (5%)
Frame = +2
Query: 347 IPSLKMEHD---GE-CQGAKLASLHPCICTREKDPVCGSDGVTYSNLCLLKCASLSKPSL 514
+PS K+ + GE C+G + P C R DPVCG D VTY C CA +
Sbjct: 47 LPSEKINGEKNRGEFCEGIAKPASCPVQCFRP-DPVCGEDSVTY--WC--GCADALCHGV 101
Query: 515 SIEHTGPCD 541
+ G CD
Sbjct: 102 RVVKQGACD 110
Score = 27.5 bits (58), Expect = 7.4
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = +2
Query: 137 PVCGSDGKTYENPCEFYCEKDKTHSNMTIVKNTACEVG 250
PVCG D TY C D + +VK AC+VG
Sbjct: 80 PVCGEDSVTYWCGC-----ADALCHGVRVVKQGACDVG 112
>At5g60920.1 68418.m07642 phytochelatin synthetase, putative / COBRA
cell expansion protein COB, putative similar to
phytochelatin synthetase GI:29570314; similar to
GB:AAK56072; identified in Roudier, et al, Plant Phys.
(2002) 130:538-548 (PMID:12376623); identical to cDNA
putative phytochelatin synthetase GI:3559804; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 456
Score = 31.1 bits (67), Expect = 0.60
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = +3
Query: 231 TPH-ARWASPATVPWS-MPPSVALTEKLTPTNVHWNAPKRLYRL*RWNMMANARELNWRV 404
TPH A SP T + +PP V T + P VHW+ K+ Y+ W + N+R+
Sbjct: 270 TPHLASVVSPPTKKGTVLPPLVQCTRHMCPIRVHWHV-KQNYKE-YWRVKITITNFNYRL 327
>At2g17620.1 68415.m02038 cyclin, putative (CYC2a) similar to cyclin
2b protein [Arabidopsis thaliana] GI:509423; contains
Pfam profiles PF00134: Cyclin, N-terminal domain,
PF02984: Cyclin, C-terminal domain; identical to cDNA
cyc2a mRNA for cyclin 2a protein GI:728518
Length = 429
Score = 31.1 bits (67), Expect = 0.60
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +2
Query: 83 YSVTALPPPLCICGKIYSPVCGSDG-KTYENPCEFYC 190
Y + PP L +Y+ C DG + + + CEF+C
Sbjct: 336 YEMLRFPPSLLAATSVYTAQCTLDGSRKWNSTCEFHC 372
>At5g05660.1 68418.m00622 zinc finger (NF-X1 type) family protein
contains PF01422: NF-X1 type zinc finger
Length = 912
Score = 29.9 bits (64), Expect = 1.4
Identities = 27/119 (22%), Positives = 45/119 (37%), Gaps = 10/119 (8%)
Frame = +2
Query: 110 LCICGKIYSPVCGSDGKTYENPCEFYCEKDKT----HSNMTIVKNTACE-----VGIPCY 262
LC CGK P + + C CE+ + H + + C V C+
Sbjct: 165 LCYCGKEEDPPADNPW-ILPHSCGEVCERPLSNNCGHCCLLLCHPGPCASCPKLVKAKCF 223
Query: 263 CT-LEYAPVCGSNGKTYANKCSLECTQKIIPSLKMEHDGECQGAKLASLHPCICTREKD 436
C +E CG + + C I ++ HDGEC + +++ C C + K+
Sbjct: 224 CGGVEDVRRCGHKQFSCGDVCERVLDCNIHNCREICHDGECPPCRERAVYKCSCGKVKE 282
>At3g17750.1 68416.m02265 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 1138
Score = 29.9 bits (64), Expect = 1.4
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -1
Query: 158 YHLTRRRDYRFFRKYITAVAELLPSIL 78
YHL R DY +FR+++ V ELL + L
Sbjct: 887 YHLLRLYDYFYFREHLLIVCELLKANL 913
>At5g55960.1 68418.m06979 expressed protein
Length = 648
Score = 29.5 bits (63), Expect = 1.8
Identities = 14/32 (43%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -1
Query: 332 IPVNICWRKFFR-*SHRRGHTPRYSSRGCPPR 240
IP N+ W++ FR S R+ P SS PPR
Sbjct: 14 IPTNLAWQEMFRSASSRKPQDPPSSSSSSPPR 45
>At3g62810.1 68416.m07057 complex 1 family protein / LVR family
protein contains Pfam PF05347: Complex 1 protein (LYR
family)
Length = 106
Score = 28.7 bits (61), Expect = 3.2
Identities = 20/61 (32%), Positives = 29/61 (47%)
Frame = +3
Query: 411 RVYARARKIQFAGATASLTAICAS*NARHCPNQA*ASNTPVLAIITECRRWETKTIRTSI 590
R RA K FAG T L A + + N+ ASN+ + ++ E R T+ I T I
Sbjct: 11 RALLRATKKSFAGDTEMLKASASEIRKKFEENRLVASNSDITRLLEEARE-ATQFISTMI 69
Query: 591 L 593
+
Sbjct: 70 V 70
>At3g61980.1 68416.m06961 serine protease inhibitor, Kazal-type
family protein contains Pfam domain PF00050: Kazal-type
serine protease inhibitor domain
Length = 117
Score = 28.7 bits (61), Expect = 3.2
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = +2
Query: 434 DPVCGSDGVTY 466
DPVCG+DGVTY
Sbjct: 52 DPVCGTDGVTY 62
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = +2
Query: 137 PVCGSDGKTYENPCEFYCEKDKTHSNMTIVKNTACEVG 250
PVCG+DG TY C D +VK AC+ G
Sbjct: 53 PVCGTDGVTYWCGC-----PDAACHGARVVKKGACDTG 85
>At1g73460.1 68414.m08504 protein kinase family protein contains
protein kinase domain Pfam:PF00069
Length = 1169
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -1
Query: 158 YHLTRRRDYRFFRKYITAVAELLPSIL 78
YHL R DY ++R+++ V ELL + L
Sbjct: 918 YHLLRLYDYFYYREHLLIVCELLKANL 944
>At1g73450.1 68414.m08503 protein kinase, putative similar to
nuclear serine/threonine protein kinase GI:3582644 from
[Rattus norvegicus]
Length = 1152
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -1
Query: 158 YHLTRRRDYRFFRKYITAVAELLPSIL 78
YHL R DY ++R+++ V ELL + L
Sbjct: 901 YHLLRLYDYFYYREHLLIVCELLKANL 927
>At5g56050.1 68418.m06993 hypothetical protein
Length = 283
Score = 27.9 bits (59), Expect = 5.6
Identities = 12/30 (40%), Positives = 16/30 (53%), Gaps = 1/30 (3%)
Frame = +3
Query: 228 KTPHARWASPATVPW-SMPPSVALTEKLTP 314
+TP ++W SP PW + P S T TP
Sbjct: 21 ETPSSKWYSPIYTPWRTTPRSTQSTPTTTP 50
>At1g61870.1 68414.m06981 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 408
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/71 (23%), Positives = 28/71 (39%), Gaps = 3/71 (4%)
Frame = +2
Query: 161 TYENPCEFYCEKDKTHSNMTIVK---NTACEVGIPCYCTLEYAPVCGSNGKTYANKCSLE 331
TY + +C +D + K N C+ CY TL Y G + +T + C
Sbjct: 294 TYSHLIHGFCNEDDFEEAKKLFKIMVNRGCKPDSECYFTLIYYLCKGGDFETALSLCKES 353
Query: 332 CTQKIIPSLKM 364
+ +PS +
Sbjct: 354 MEKNWVPSFSI 364
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -1
Query: 584 CSNGFSFPPPTLCYYRKDRCVRCSSL 507
C GF PPT YR+D C L
Sbjct: 1192 CRKGFRHEPPTNVDYRRDMCAHARVL 1217
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -1
Query: 584 CSNGFSFPPPTLCYYRKDRCVRCSSL 507
C GF PPT YR+D C L
Sbjct: 1192 CRKGFRHEPPTNVDYRRDMCAHARVL 1217
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -1
Query: 584 CSNGFSFPPPTLCYYRKDRCVRCSSL 507
C GF PPT YR+D C L
Sbjct: 1192 CRKGFRHEPPTNVDYRRDMCAHARVL 1217
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -1
Query: 584 CSNGFSFPPPTLCYYRKDRCVRCSSL 507
C GF PPT YR+D C L
Sbjct: 1192 CRKGFRHEPPTNVDYRRDMCAHARVL 1217
>At5g42620.1 68418.m05188 expressed protein
Length = 841
Score = 27.1 bits (57), Expect = 9.7
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +2
Query: 284 VCGSNGKTYANKC--SLECTQKIIPSLKMEHDGECQGA 391
VC S ++Y C SL+C+ + + S E D EC G+
Sbjct: 786 VCHSACQSYNMACGASLDCSDQTLFSTAEEGDAECTGS 823
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,924,622
Number of Sequences: 28952
Number of extensions: 343388
Number of successful extensions: 877
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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