Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_G13
(632 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 217 4e-57
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 217 7e-57
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 196 1e-50
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 109 1e-24
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 95 5e-20
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 85 4e-17
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 0.64
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.1
At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing p... 29 2.6
At2g43440.1 68415.m05399 F-box family protein contains Pfam PF00... 29 3.4
At1g58210.1 68414.m06610 kinase interacting family protein simil... 28 4.5
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 28 4.5
At5g33380.1 68418.m03963 hypothetical protein 27 7.8
At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family... 27 7.8
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 217 bits (531), Expect = 4e-57
Identities = 103/181 (56%), Positives = 129/181 (71%), Gaps = 2/181 (1%)
Frame = +3
Query: 96 VFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYALS 269
V FEE+F DD WE+ WV SE + G++K TAG + DA DKG+QTSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFYAIS 81
Query: 270 RKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDICG 449
+F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 450 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGELEA 629
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +LIDN + ++G L +
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDNVEKQTGSLYS 201
Query: 630 D 632
D
Sbjct: 202 D 202
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 217 bits (529), Expect = 7e-57
Identities = 101/184 (54%), Positives = 130/184 (70%), Gaps = 2/184 (1%)
Frame = +3
Query: 87 NCEVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFY 260
+ EV FEEKF +D WE WV S+ + G++K TAG + DA DKG+QTSED RFY
Sbjct: 21 SAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFY 78
Query: 261 ALSRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPD 440
A+S +F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQ G+TPY IMFGPD
Sbjct: 79 AISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGPD 138
Query: 441 ICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGE 620
ICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +LIDN + ++G
Sbjct: 139 ICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTGS 198
Query: 621 LEAD 632
L +D
Sbjct: 199 LYSD 202
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 196 bits (478), Expect = 1e-50
Identities = 92/182 (50%), Positives = 126/182 (69%), Gaps = 2/182 (1%)
Frame = +3
Query: 93 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 266
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 267 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDIC 446
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 447 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGELE 626
G TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y VL+DN++ E G +
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMY 206
Query: 627 AD 632
D
Sbjct: 207 TD 208
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 109 bits (262), Expect = 1e-24
Identities = 54/114 (47%), Positives = 75/114 (65%), Gaps = 2/114 (1%)
Frame = +3
Query: 93 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 266
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 267 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIM 428
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 94.7 bits (225), Expect = 5e-20
Identities = 57/178 (32%), Positives = 92/178 (51%), Gaps = 10/178 (5%)
Frame = +3
Query: 120 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF-KPFSNE 296
D+ ++ W+ S++ E G +K ++ D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 297 GKPLVVQFSVKHEQDIDCGGGYLKVF---DCRLDQKDMHGETPYEIMFGPDICGPGTKKV 467
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 468 HVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGEL 623
H I +K H +K D +H+YT I+KPDN +L+D E+ + L
Sbjct: 150 HFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKPDNEVRILVDGEEKKKANL 207
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 85.0 bits (201), Expect = 4e-17
Identities = 56/181 (30%), Positives = 93/181 (51%), Gaps = 13/181 (7%)
Frame = +3
Query: 120 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF---KPFS 290
D+ ++ WV SE + G +K + ++D GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEKSEGHDDY----GLLVSEKAKKYGIVKELDVDEPLN 90
Query: 291 -NEGKPLVVQFSVKHEQDIDCGGGYLKVF---DCRLDQKDMHGETPYEIMFGPDICGPGT 458
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 459 KKVHVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGE 620
KVH I +K H +K D+ +H+YT ++K DN +L+D E+ + G
Sbjct: 149 NKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKSDNEVRILVDGEEKKKGN 208
Query: 621 L 623
L
Sbjct: 209 L 209
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 0.64
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 312 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEI 425
++F+ KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.1
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 312 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYE 422
++F+ KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing
protein / plus-3 domain-containing protein / GYF
domain-containing protein similar to CPRF interacting
protein [Petroselinum crispum] GI:9588690; contains Pfam
profiles PF02201: BAF60b domain of the SWIB complex,
PF03126: Plus-3 domain, PF02213: GYF domain; contains
non-conensus AT-AC splice sites at intron 5
Length = 553
Score = 29.1 bits (62), Expect = 2.6
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 117 SDDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAE 218
S D + NW+Y + G G F LT K ++DAE
Sbjct: 492 SYDKEKLNWLYKDPQGLVQGPFSLTQLKAWSDAE 525
>At2g43440.1 68415.m05399 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 791
Score = 28.7 bits (61), Expect = 3.4
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = -1
Query: 434 PKHNLVGCLAMHVLLVQSTVEYLQVTASAVDVLFVLHTELYHQRFALIAEWL 279
P HN++ + V ++ +LQ++A + + LH E YH L A W+
Sbjct: 184 PPHNMLP--GSKSVCVNGSIYWLQISAGYIILAMDLHEESYHGVHHLPATWV 233
>At1g58210.1 68414.m06610 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) {Petunia
integrifolia}
Length = 1246
Score = 28.3 bits (60), Expect = 4.5
Identities = 15/29 (51%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +3
Query: 210 DAEADKGLQTSEDARFYALSRKF-KPFSN 293
+AE +KG T+ RFYAL KF KP S+
Sbjct: 583 EAEIEKGRITTAKERFYALRNKFEKPESD 611
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 28.3 bits (60), Expect = 4.5
Identities = 18/63 (28%), Positives = 24/63 (38%)
Frame = -2
Query: 202 NFPAVNLNLPNSFPGCSLYTQFDSHESSENFSSKNTSQFIDEIANKLAIASTADFIF*FT 23
N+P + + + CS D SS NF D++ N L I D I F
Sbjct: 31 NYPDYKIVVLDKLDYCSDLKNLDPSFSSPNFKFVKGDIASDDLVNYLLITENIDTIMHFA 90
Query: 22 AST 14
A T
Sbjct: 91 AQT 93
>At5g33380.1 68418.m03963 hypothetical protein
Length = 154
Score = 27.5 bits (58), Expect = 7.8
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = -3
Query: 390 GPVYSRIPSGNRLRSRCPVRASH*TVPPKVCPHC*MA*ISSIG 262
G + +RI + R P R T+ P+V HC ISS G
Sbjct: 111 GDLRARIGFSVIVTHRVPTREKEQTISPRVSSHCARGSISSFG 153
>At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family
protein
Length = 466
Score = 27.5 bits (58), Expect = 7.8
Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 396 DMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKK-DIRCKDDVYTHLYTL 557
D+ + + P+I G G K V +++ + K +KK ++ KD T LY L
Sbjct: 152 DIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGFLKKLSLKFKDPENTTLYIL 206
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,872,456
Number of Sequences: 28952
Number of extensions: 298745
Number of successful extensions: 800
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 791
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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