Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_G11
(294 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g28520.1 68414.m03506 expressed protein 28 0.94
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 28 1.2
At5g48010.1 68418.m05933 pentacyclic triterpene synthase, putati... 27 1.6
At2g11090.1 68415.m01187 expressed protein 27 2.9
At5g36150.1 68418.m04356 pentacyclic triterpene synthase, putati... 26 3.8
At4g15370.1 68417.m02349 pentacyclic triterpene synthase, putati... 26 3.8
At2g13770.1 68415.m01521 hypothetical protein 26 3.8
At1g74790.1 68414.m08665 expressed protein contains similarity t... 26 3.8
At1g72550.2 68414.m08390 tRNA synthetase beta subunit family pro... 26 3.8
At1g72550.1 68414.m08389 tRNA synthetase beta subunit family pro... 26 3.8
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 26 5.0
At1g78500.1 68414.m09150 pentacyclic triterpene synthase, putati... 25 6.6
At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 25 6.6
At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 25 6.6
At5g57720.1 68418.m07215 transcriptional factor B3 family protei... 25 8.7
At3g57010.1 68416.m06346 strictosidine synthase family protein s... 25 8.7
>At1g28520.1 68414.m03506 expressed protein
Length = 486
Score = 28.3 bits (60), Expect = 0.94
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +2
Query: 44 NTTNNGPDCMKGTKDCAHPVVTAPIEKTI 130
NTTNN C+KG + V T ++ T+
Sbjct: 433 NTTNNNKRCIKGRPKVSTKVATGNVQNTV 461
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar
to SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.9 bits (59), Expect = 1.2
Identities = 16/36 (44%), Positives = 20/36 (55%)
Frame = +2
Query: 80 TKDCAHPVVTAPIEKTIPHPDYIPNDVQGRHDIALI 187
TK C V TAPI KTI + Y D G+ D+ L+
Sbjct: 391 TKHCTRAVYTAPI-KTISNQKY--RDFCGKFDVGLL 423
>At5g48010.1 68418.m05933 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650207]
[PMID: 11247608] Contains Pfam domain PF00432:
Prenyltransferase and squalene oxidase repeat
Length = 758
Score = 27.5 bits (58), Expect = 1.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 184 KRNVMSALHIVGDVIWMWNSFFYRSSNNGMCTVFGTFHAVRTIV 53
KR + A + D+ + S++ N G+C ++GTF AVR +V
Sbjct: 586 KRFITKAAKYIEDMQTVDGSWY---GNWGVCFIYGTFFAVRGLV 626
>At2g11090.1 68415.m01187 expressed protein
Length = 151
Score = 26.6 bits (56), Expect = 2.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 200 TAPYTDFVRPICLPSLDYTQQPPGR 274
+ P++ + PSLD+T QP GR
Sbjct: 52 STPWSSIITSTTSPSLDHTTQPRGR 76
>At5g36150.1 68418.m04356 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650208]
[PMID:11247608]; oxidosqualene cyclase; also highly
similar to beta-amyrin synthase, lupeol synthase,
cycloartenol synthase
Length = 729
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -3
Query: 106 NNGMCTVFGTFHAVRTIV 53
N G+C ++GTF AVR +V
Sbjct: 612 NWGICFIYGTFFAVRGLV 629
>At4g15370.1 68417.m02349 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650208]
[PMID:11247608]
Length = 759
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -3
Query: 106 NNGMCTVFGTFHAVRTIV 53
N G+C ++GTF AVR +V
Sbjct: 612 NWGVCFIYGTFFAVRGLV 629
>At2g13770.1 68415.m01521 hypothetical protein
Length = 244
Score = 26.2 bits (55), Expect = 3.8
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 12/49 (24%)
Frame = -3
Query: 205 CCY-HQPNKRNVMSALHI---------VGDV-IWMWNSFF-YRSSNNGM 95
CCY P+ +V LHI V D +W+W+++F SNNG+
Sbjct: 45 CCYLRSPDANDVARLLHIGESRGFPEAVADYDLWIWHAYFGLPGSNNGI 93
>At1g74790.1 68414.m08665 expressed protein contains similarity to
hedgehog-interacting protein GI:4868122 from [Mus
musculus]
Length = 695
Score = 26.2 bits (55), Expect = 3.8
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 4/43 (9%)
Frame = +1
Query: 91 CTSRCYCSYRKNYSTSRLHP----QRCARQT*HCAY*ADGNST 207
CT RC C+ N S+L P Q C QT Y A+ S+
Sbjct: 299 CTGRCSCNSDVNCDPSKLTPDSGSQPCQYQTVIAEYTANSTSS 341
>At1g72550.2 68414.m08390 tRNA synthetase beta subunit family
protein contains Pfam profiles: PF03484
phenylalanine-tRNA synthetase, B5 domain, PF03483 B3/4
domain; an isoform contains a non-consensus TG acceptor
splice site at a terminal exon.
Length = 584
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 72 MQSGPLFVVLYSPKRTFFGVPSI 4
+++ P+F VLY KRT +P I
Sbjct: 215 IENSPVFPVLYDSKRTVLSLPPI 237
>At1g72550.1 68414.m08389 tRNA synthetase beta subunit family
protein contains Pfam profiles: PF03484
phenylalanine-tRNA synthetase, B5 domain, PF03483 B3/4
domain; an isoform contains a non-consensus TG acceptor
splice site at a terminal exon.
Length = 598
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 72 MQSGPLFVVLYSPKRTFFGVPSI 4
+++ P+F VLY KRT +P I
Sbjct: 215 IENSPVFPVLYDSKRTVLSLPPI 237
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 25.8 bits (54), Expect = 5.0
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2
Query: 263 VVAYNPKTVNRWA 225
VV NP+TVNRWA
Sbjct: 559 VVVPNPETVNRWA 571
>At1g78500.1 68414.m09150 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase (04C11)
[gi:6650208] [PMID:11247608]; similar to beta-Amyrin
Synthase GI:3688600 from [Panax ginseng]
Length = 767
Score = 25.4 bits (53), Expect = 6.6
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -3
Query: 106 NNGMCTVFGTFHAVRTIV 53
N G+C ++GTF AVR +V
Sbjct: 618 NWGVCFMYGTFFAVRGLV 635
>At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 662
Score = 25.4 bits (53), Expect = 6.6
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 233 CLPSLDYTQQPP 268
CLP LD +QQPP
Sbjct: 149 CLPPLDMSQQPP 160
>At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 665
Score = 25.4 bits (53), Expect = 6.6
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 233 CLPSLDYTQQPP 268
CLP LD +QQPP
Sbjct: 149 CLPPLDMSQQPP 160
>At5g57720.1 68418.m07215 transcriptional factor B3 family protein
contains Pfam profile PF02362: B3 DNA binding domain
Length = 300
Score = 25.0 bits (52), Expect = 8.7
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +2
Query: 131 PHPDYIPNDVQGRHDIALIRLMVTAPYTDFVRPICLPSLDYTQQPPGRFW 280
P+PD++ + H+ + + L++ Y + P LP + P GRFW
Sbjct: 9 PYPDFLK--IFNSHEDSQL-LVIPRSYNRYY-PNPLPQTAVLKNPEGRFW 54
>At3g57010.1 68416.m06346 strictosidine synthase family protein
similar to strictosidine synthase [Rauvolfia
serpentina][SP|P15324]
Length = 376
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +2
Query: 59 GPDCMKGTKDCAHPVVTAPIEKTIPHPDYIPNDVQG 166
GPD +KGTK T P+ + P+ + D QG
Sbjct: 31 GPDNLKGTKHVLQDAKTIPL--PVDGPESLEFDPQG 64
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,490,736
Number of Sequences: 28952
Number of extensions: 154475
Number of successful extensions: 386
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 386
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 271185840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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