BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_G03 (540 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 97 2e-21 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 73 2e-14 SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 67 1e-12 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 58 8e-10 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 54 2e-08 SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 29 0.58 SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 26 3.1 SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces... 25 5.4 SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy... 25 7.2 SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 25 7.2 SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 25 9.5 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 9.5 SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 25 9.5 SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr... 25 9.5 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 96.7 bits (230), Expect = 2e-21 Identities = 56/165 (33%), Positives = 85/165 (51%), Gaps = 5/165 (3%) Frame = +3 Query: 57 VNNYPLPKITKDTDVIVKVEYSGVCGTDLHIIQ----GEFPALKDRPFPLGHESSGIIHE 224 + + P +T D V V ++ +G+CG+D+H + G+F + +P LGHES+G++ E Sbjct: 18 IEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDF--ILKKPMILGHESAGVVVE 75 Query: 225 VGTA-SIFKKGQKVAVDPNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDGGWAQYVCVP 401 VG S K G VAV+P C LC++CRSG Y C +T DG Y Sbjct: 76 VGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPY--DGTLRTYYITT 133 Query: 402 QDQVYALPDGITTEQAGLCEPYSCVSHGFKRASPVSVGKKILIVG 536 +D LP I+ E+ L EP S H R + + G ++L++G Sbjct: 134 EDFCTKLPKQISVEEGALFEPMSVAVHAMTRGN-LKCGSRVLVMG 177 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 73.3 bits (172), Expect = 2e-14 Identities = 46/152 (30%), Positives = 73/152 (48%), Gaps = 4/152 (2%) Frame = +3 Query: 96 DVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVGT-ASIFKKGQKVAVD 272 +V+V ++Y+GVC TDLH +QG++P P GHE +G++ +VG + K G +V V Sbjct: 35 EVLVNIKYTGVCHTDLHALQGDWPLPAKMPLIGGHEGAGVVVKVGAGVTRLKIGDRVGVK 94 Query: 273 -PNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDGGWAQYVCVPQDQVYALPDGITTEQA 449 N +C CE+C C ++ G DG + Y +P+ + E A Sbjct: 95 WMNSSCGNCEYCMKAEETICPHIQLS---GYTVDGTFQHYCIANATHATIIPESVPLEVA 151 Query: 450 G--LCEPYSCVSHGFKRASPVSVGKKILIVGA 539 +C +C + + S V G+ I I GA Sbjct: 152 APIMCAGITC--YRALKESKVGPGEWICIPGA 181 >SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 67.3 bits (157), Expect = 1e-12 Identities = 40/110 (36%), Positives = 56/110 (50%), Gaps = 2/110 (1%) Frame = +3 Query: 12 MEAVLFDGKTSTLKYVNNYPLPKITKDTDVIVKVEYSGVC-GTDLHIIQGEFPALKDRPF 188 M+A ++DG + + P P IT DVIVK +C G+D HI GE P ++ Sbjct: 37 MKACVWDGPLNVK--IAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94 Query: 189 PLGHESSGIIHEVGT-ASIFKKGQKVAVDPNRACNLCEFCRSGNYQYCLT 335 LGHES GI+ E G + + G +V + + AC C FC+ Y C T Sbjct: 95 -LGHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDT 143 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 58.0 bits (134), Expect = 8e-10 Identities = 34/97 (35%), Positives = 46/97 (47%), Gaps = 1/97 (1%) Frame = +3 Query: 87 KDTDVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVGTASI-FKKGQKV 263 K +V VKV++S VC TD + + G P P LGHE +GI+ +G I + G V Sbjct: 33 KAHEVRVKVDWSAVCHTDAYTLSGVDPE-GAFPIVLGHEGAGIVESIGEGVINVRPGDHV 91 Query: 264 AVDPNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDG 374 + C C+FCRSG C G+ DG Sbjct: 92 ILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDG 128 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 53.6 bits (123), Expect = 2e-08 Identities = 31/94 (32%), Positives = 44/94 (46%), Gaps = 1/94 (1%) Frame = +3 Query: 96 DVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVG-TASIFKKGQKVAVD 272 +V +K+ SGVC TD + + G+ P P LGHE +GI+ VG + + G V Sbjct: 39 EVRIKIVNSGVCHTDAYTLSGKDPE-GLFPVILGHEGAGIVESVGPQVTTVQVGDPVIAL 97 Query: 273 PNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDG 374 C C+FC+SG C G+ DG Sbjct: 98 YTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDG 131 >SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 28.7 bits (61), Expect = 0.58 Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 1/65 (1%) Frame = +3 Query: 348 STIGIWKDGGWAQYVCVPQDQVYALPDGIT-TEQAGLCEPYSCVSHGFKRASPVSVGKKI 524 S + KDG A+Y +P+ +P ++ TE A + P++ R + Sbjct: 94 SELSFTKDGTHAEYCIIPEKAAVRMPSNLSFTEAASVGVPFTTAYLALSRGE-TKGSDIV 152 Query: 525 LIVGA 539 L+VGA Sbjct: 153 LVVGA 157 >SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1150 Score = 26.2 bits (55), Expect = 3.1 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +1 Query: 46 PLNTSTIILYPRLQKTRMS*SKWNTL 123 PL + I+LY R Q TRM ++WN L Sbjct: 386 PLCCAKILLYARRQLTRM--ARWNNL 409 >SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 796 Score = 25.4 bits (53), Expect = 5.4 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 4/50 (8%) Frame = +3 Query: 228 GTASIFKKGQKVA----VDPNRACNLCEFCRSGNYQYCLTAGINSTIGIW 365 GT ++ G VA +R N + +G+ Y +TAG ++ I +W Sbjct: 164 GTVKVWSFGSSVANFTLQAHDRGVNYVNYYPAGDKPYLITAGDDNLIKVW 213 >SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 922 Score = 25.0 bits (52), Expect = 7.2 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +3 Query: 303 CRSGNYQYCLTAGINSTIGIWK 368 C S + + CLTAG S + +W+ Sbjct: 222 CVSPDAKRCLTAGTGSDVKVWE 243 >SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 900 Score = 25.0 bits (52), Expect = 7.2 Identities = 10/37 (27%), Positives = 22/37 (59%) Frame = +3 Query: 186 FPLGHESSGIIHEVGTASIFKKGQKVAVDPNRACNLC 296 F +G ++S + HE+ T + + + + ++PN +C C Sbjct: 805 FEIG-QASEVAHEIQTNAENMRNRYIVLEPNESCWHC 840 >SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 9.5 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +3 Query: 18 AVLFDGKTSTLKYVNNYPLPKITK 89 A +FDG++ST+K V N P+ + Sbjct: 638 AFIFDGESSTMKLVGNPSGPEFER 661 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 24.6 bits (51), Expect = 9.5 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +3 Query: 423 PDGITTEQAGLCEPYSCVSHGFKRASPVSVGKKIL 527 PD T + G+ PY H +RA V GK + Sbjct: 1525 PDVNFTGRIGVITPYRSQLHELRRAFKVKYGKSFM 1559 >SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 506 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = +1 Query: 97 MS*SKWNTLESAVLISILFRVNS 165 M S+W TL + +LIS L+ VN+ Sbjct: 1 MKFSQWYTLTAPLLISSLYTVNA 23 >SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/40 (27%), Positives = 22/40 (55%) Frame = -2 Query: 161 FTLNNMEISTADSRVFHFDYDIRVFCNLG*RIIVDVFKGR 42 + L +E + VF+ D +I++ NL ++VD +G+ Sbjct: 556 YNLYLVEFKAGRADVFYIDDNIKLSLNLNDYVVVDADRGQ 595 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,266,526 Number of Sequences: 5004 Number of extensions: 45906 Number of successful extensions: 151 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 221892220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -