Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_F17
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 246 3e-66
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 31 0.18
SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 30 0.24
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 28 0.95
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 28 1.3
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 26 5.1
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 26 5.1
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 25 6.7
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 6.7
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 8.9
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 25 8.9
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 8.9
SPAC22A12.06c |||serine hydrolase|Schizosaccharomyces pombe|chr ... 25 8.9
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 246 bits (601), Expect = 3e-66
Identities = 113/190 (59%), Positives = 142/190 (74%)
Frame = +2
Query: 2 RFKDIFEEVYQSDYKKQFDDAKIWYEHRLIDDMVAQAIKGSGGFVWACKNYDGDVQSDVV 181
RFKD F+EVY+SDYK++F++ +WY+HRLIDDMVAQAIK +GGFVWACKNYDGDV SDVV
Sbjct: 232 RFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSNGGFVWACKNYDGDVMSDVV 291
Query: 182 AQGYGSLGMMTSVLMCPDGRTVESEAAHGTVTRHYRMHQQGKPTSTNPVASIYAWTRGLV 361
AQ YGSLG+MTSVL+ P+GRT ESEAAHGTV RHY + +GK TSTN +ASI+AWTRGL
Sbjct: 292 AQAYGSLGLMTSVLIHPNGRTFESEAAHGTVQRHYMQYLKGKKTSTNSIASIFAWTRGLA 351
Query: 362 HRAKLDGTPXXXXXXXXXXXXCVECIDSGKMTKDLVICIHGLANTKEGMYLNTEDFLQAI 541
HR +LDG CV C++ G MTKDL + L+ + G Y++T +FL A+
Sbjct: 352 HRGRLDGNERLVKFANALEHACVRCVEKGIMTKDLYL----LSKSPNG-YVDTFEFLDAV 406
Query: 542 ADQLERKLSN 571
+L+ +L N
Sbjct: 407 KSELDSELVN 416
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 30.7 bits (66), Expect = 0.18
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +2
Query: 62 AKIWYEHRLIDDMVAQAIKGSGGF-VWACKNYDGDVQSDVVAQGYGSLGMMTSVLMCPDG 238
A I + +++D MV + + F V N GD+ SD A GSLG++ S +
Sbjct: 222 ASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV--GD 279
Query: 239 RTVESEAAHGT 271
V SE HG+
Sbjct: 280 NFVMSEPVHGS 290
>SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 30.3 bits (65), Expect = 0.24
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = -1
Query: 179 LHRTARHRRNSYRPIRIHRILLSPVRPYHQSAYAHTKS 66
LH+ +H NSYRP R + +H+S Y+ +S
Sbjct: 182 LHKNHKHYENSYRPSRSQNNSHYNDKSFHRSRYSRARS 219
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 28.3 bits (60), Expect = 0.95
Identities = 19/71 (26%), Positives = 29/71 (40%)
Frame = -1
Query: 350 SSKRISRRRGSWTSASLVGACDSAESRCRERLQTPPSVRQDTSVLTSSFPANRNPALLHR 171
SS S S TS+S + S + S + +S + S + + HR
Sbjct: 121 SSSSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSSRSSSRTTSHR 180
Query: 170 TARHRRNSYRP 138
T H+ +SYRP
Sbjct: 181 TTSHKSSSYRP 191
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 27.9 bits (59), Expect = 1.3
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = +2
Query: 149 NYDGDVQSDVVAQGYGSLGMMTSVLMCPDGRTVESEAAHGTV 274
N+ G + V +QG+ + + S LMCP + E H ++
Sbjct: 244 NFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSI 285
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 25.8 bits (54), Expect = 5.1
Identities = 23/84 (27%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +2
Query: 8 KDIFEEVYQSDYKKQFDDAKIWYEH-RLIDDMVAQAIKGSGGFVWACKNYDGDVQSDVVA 184
K I+E + F AK W H DD+ + G V+ KNYDGDV++D+
Sbjct: 774 KCIYEGIQIDAAFASFFVAK-WLGHPSYFDDLTSLDPNLYEGLVFL-KNYDGDVENDMAL 831
Query: 185 Q---GYGSLGMMTSVLMCPDGRTV 247
+ G+ + + P+G +
Sbjct: 832 NFTVVHEEFGVRNVIDLIPNGSNI 855
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 25.8 bits (54), Expect = 5.1
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 479 HGLANTKEGMYLNTEDFLQAIADQLERKLS 568
HGL T E + NTE L +QLE+K+S
Sbjct: 244 HGL--TPEVFWQNTESILTCPEEQLEQKIS 271
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 25.4 bits (53), Expect = 6.7
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = -1
Query: 422 PLQGRVQTAPVLEYRPA*HGEPNPSSKRISRRRGSWTSASLVGACDSAESRCRERLQTPP 243
PLQ +V TA + P +P++ R S R+G+ + + ++ + + PP
Sbjct: 145 PLQSKVTTASLKPNIPRTSMSSSPTASRTSLRKGAGELETHSAVKSAKKASVKSKSVLPP 204
Query: 242 S 240
S
Sbjct: 205 S 205
>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 476
Score = 25.4 bits (53), Expect = 6.7
Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = -1
Query: 266 RERLQTPPSVRQDTSVLTSSFPANRNPALLHRTARHR-RNSY 144
RE L PSVR+ + S+P++ RHR NSY
Sbjct: 142 REMLPPQPSVRRPRFYSSYSYPSSHQDPAYSSFKRHRSSNSY 183
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.0 bits (52), Expect = 8.9
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +3
Query: 528 SYRQSPISWNANY 566
SY +SPISWN Y
Sbjct: 336 SYIESPISWNLKY 348
>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 604
Score = 25.0 bits (52), Expect = 8.9
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -1
Query: 176 HRTARHRRNSYRP 138
HRT RH R SY P
Sbjct: 110 HRTPRHHRRSYSP 122
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 25.0 bits (52), Expect = 8.9
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 354 VWFTVLSWTVLQNWSGLHSPLKR 422
V+ TV W++ QNWS + KR
Sbjct: 69 VYQTVRHWSLKQNWSSMSLDQKR 91
>SPAC22A12.06c |||serine hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 429
Score = 25.0 bits (52), Expect = 8.9
Identities = 10/34 (29%), Positives = 18/34 (52%)
Frame = +2
Query: 455 TKDLVICIHGLANTKEGMYLNTEDFLQAIADQLE 556
TK ++CIHG A + E + + +AD ++
Sbjct: 5 TKSKILCIHGYAESGELFSVKLRALRERMADSVD 38
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,729,641
Number of Sequences: 5004
Number of extensions: 60515
Number of successful extensions: 181
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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