BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_F15 (308 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g17490.1 68416.m02234 F-box family protein similar to F-box p... 35 0.013 At3g17480.1 68416.m02233 F-box family protein contains F-box dom... 33 0.052 At2g23200.1 68415.m02771 protein kinase family protein contains ... 28 1.1 At3g61160.2 68416.m06845 shaggy-related protein kinase beta / AS... 27 1.9 At3g61160.1 68416.m06844 shaggy-related protein kinase beta / AS... 27 1.9 At2g02500.1 68415.m00189 expressed protein contains Pfam profile... 27 2.6 At1g71940.1 68414.m08316 expressed protein 27 2.6 At3g17620.1 68416.m02251 F-box family protein contains Pfam prof... 27 3.4 At3g13830.1 68416.m01746 F-box family protein contains Pfam:PF00... 27 3.4 At2g17220.2 68415.m01989 protein kinase, putative similar to pro... 27 3.4 At1g74580.1 68414.m08639 pentatricopeptide (PPR) repeat-containi... 27 3.4 At3g17530.1 68416.m02239 F-box family protein contains Pfam prof... 26 4.5 At3g16740.1 68416.m02137 F-box family protein contains F-box dom... 26 4.5 At3g03810.1 68416.m00391 expressed protein contains Pfam PF03138... 26 4.5 At1g32140.1 68414.m03954 F-box family protein contains F-box dom... 26 4.5 At5g46570.1 68418.m05734 protein kinase family protein contains ... 26 5.9 At5g44670.1 68418.m05473 expressed protein contains Pfam:PF01697... 26 5.9 At5g06580.1 68418.m00743 FAD linked oxidase family protein simil... 26 5.9 At3g16250.1 68416.m02050 ferredoxin-related contains Pfam profil... 26 5.9 At1g33610.1 68414.m04160 leucine-rich repeat family protein cont... 26 5.9 At1g29355.1 68414.m03589 expressed protein 26 5.9 At5g48620.1 68418.m06013 disease resistance protein (CC-NBS-LRR ... 25 7.8 At4g16560.1 68417.m02505 heat shock protein-related contains sim... 25 7.8 At4g16550.1 68417.m02504 heat shock protein-related contains sim... 25 7.8 At3g63140.1 68416.m07091 mRNA-binding protein, putative similar ... 25 7.8 At3g54550.1 68416.m06036 hypothetical protein contains Pfam prof... 25 7.8 At3g17540.1 68416.m02240 F-box family protein similar to hypothe... 25 7.8 At1g73680.1 68414.m08532 pathogen-responsive alpha-dioxygenase, ... 25 7.8 >At3g17490.1 68416.m02234 F-box family protein similar to F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis thaliana]; contains Pfam PF00646: F-box domain and TIGRFAM TIGR01640: F-box protein interaction domain Length = 388 Score = 34.7 bits (76), Expect = 0.013 Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 1/63 (1%) Frame = -3 Query: 306 PSVPLG-NINLFGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGV 130 P LG + F ++S S+++ ++ + + GK SSL N FE+ I C G+ Sbjct: 51 PKTYLGLTLKDFRIYSMSSNLHGLLHNNNIDLLMEFKGKLSSLNDLNDFEISQIYPCDGL 110 Query: 129 ILC 121 ILC Sbjct: 111 ILC 113 >At3g17480.1 68416.m02233 F-box family protein contains F-box domain Pfam:PF00646 Length = 373 Score = 32.7 bits (71), Expect = 0.052 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +2 Query: 59 NGFPITLEENWHKFYELDWFTHKITPGQNKIVRNSNEFSLFKED 190 N T+ +W KF +D +TH+ G + N+ ++F ED Sbjct: 286 NDITDTIVTSWSKFLRVDLYTHRFYNGVTFFIDEENKAAVFSED 329 >At2g23200.1 68415.m02771 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 834 Score = 28.3 bits (60), Expect = 1.1 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 3/67 (4%) Frame = +2 Query: 2 SDIATDAVIKIFLGPKYNDNGFPITLEENWHKFYELDWFTHKITPGQNKIVRN---SNEF 172 S +A I++F P +D P ++N H Y L+ KITP + + R ++ Sbjct: 177 SSLALINAIEVFSAP--DDLEIPSASDKNLHTIYRLNVGGEKITPDNDTLGRTWLPDDDD 234 Query: 173 SLFKEDS 193 L+++DS Sbjct: 235 FLYRKDS 241 >At3g61160.2 68416.m06845 shaggy-related protein kinase beta / ASK-beta (ASK2) identical to shaggy-related protein kinase beta SP:O23145 GI:2569931 from [Arabidopsis thaliana] Length = 438 Score = 27.5 bits (58), Expect = 1.9 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = +2 Query: 38 LGPKYNDNGFPITLEENWHKFY 103 + P+YND FP + WHK + Sbjct: 337 MNPRYNDFKFPQIKAQPWHKIF 358 >At3g61160.1 68416.m06844 shaggy-related protein kinase beta / ASK-beta (ASK2) identical to shaggy-related protein kinase beta SP:O23145 GI:2569931 from [Arabidopsis thaliana] Length = 431 Score = 27.5 bits (58), Expect = 1.9 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = +2 Query: 38 LGPKYNDNGFPITLEENWHKFY 103 + P+YND FP + WHK + Sbjct: 330 MNPRYNDFKFPQIKAQPWHKIF 351 >At2g02500.1 68415.m00189 expressed protein contains Pfam profile: PF01128 uncharacterized protein family UPF0007; identical to GP:12697583 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase {Arabidopsis thaliana}; identical to cDNA 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase (ISPD) GI:7385140 Length = 302 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/30 (36%), Positives = 20/30 (66%) Frame = +2 Query: 170 FSLFKEDSLPLTDLMKLLDEGKVPLYMSEG 259 F L K + L +TD + +++ K P+Y+S+G Sbjct: 249 FELVKSEGLEVTDDVSIVEYLKHPVYVSQG 278 >At1g71940.1 68414.m08316 expressed protein Length = 272 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = -3 Query: 135 GVILCV-NQSSS*NLCQFSSNVMGNPLSLYLGPKKI 31 GV+L V N ++ C F S ++G PL +L P K+ Sbjct: 131 GVVLVVFNATAGATSCFFLSKLIGRPLITWLWPDKL 166 >At3g17620.1 68416.m02251 F-box family protein contains Pfam profile: PF00646 F-box domain Length = 398 Score = 26.6 bits (56), Expect = 3.4 Identities = 19/63 (30%), Positives = 31/63 (49%) Frame = -3 Query: 300 VPLGNINLFGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGVILC 121 V L +INL G+H NG PS N + V SL + ++ + C G++LC Sbjct: 67 VYLTSINLRGIH-------NGFDPSINRQAKLV-----SLNGTDQIDISEVYHCDGLLLC 114 Query: 120 VNQ 112 +++ Sbjct: 115 ISK 117 >At3g13830.1 68416.m01746 F-box family protein contains Pfam:PF00646 F-box domain; contains TIGRFAM TIGR01640 : F-box protein interaction domain Length = 377 Score = 26.6 bits (56), Expect = 3.4 Identities = 15/59 (25%), Positives = 32/59 (54%) Frame = -3 Query: 204 VNGKESSLKSENSFELRTILFCPGVILCVNQSSS*NLCQFSSNVMGNPLSLYLGPKKIL 28 V+ + + + + FE+ + C G++LCV+ N +S+ VM ++YLG +++ Sbjct: 83 VDTSVNQISAFDDFEISQLFHCDGLLLCVS-----NKHYYSNTVM--VCNMYLGETRLI 134 >At2g17220.2 68415.m01989 protein kinase, putative similar to protein kinase APK1A [Arabidopsis thaliana] Swiss-Prot:Q06548 Length = 413 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = -3 Query: 264 SKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFEL 157 S +I +GTF SSNN + + S++ S + F + Sbjct: 18 SSTGNISSGTFKSSNNTTTTGTSRGSNISSNSGFSV 53 >At1g74580.1 68414.m08639 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile: PF01535 PPR repeat Length = 763 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = -3 Query: 249 IYNGTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGV 130 I G FP F ++G + LK EN+ E+ ++ GV Sbjct: 453 ISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDNGV 492 >At3g17530.1 68416.m02239 F-box family protein contains Pfam profile: PF00646 F-box domain Length = 388 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/34 (32%), Positives = 15/34 (44%) Frame = -3 Query: 204 VNGKESSLKSENSFELRTILFCPGVILCVNQSSS 103 V GK L ++ I C G+ILC S+ Sbjct: 83 VTGKLIKLNDSKGVDISAIFHCDGLILCTTTEST 116 >At3g16740.1 68416.m02137 F-box family protein contains F-box domain Pfam:PF00646 Length = 391 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = -3 Query: 198 GKESSLKSENSFELRTILFCPGVILCVNQSSS 103 GK SL E+ ++ I C G++LC+ + S Sbjct: 89 GKLISLDVEDHVDISKIFHCGGLLLCITKDIS 120 >At3g03810.1 68416.m00391 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'auxin-independent growth promoter protein -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 656 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = +2 Query: 80 EENWHKFYELDWFTHKITPGQNKIVRNSNEFSLFK 184 EE + F + D K P K R NEF LFK Sbjct: 149 EEQFIAFLKNDVIVMKTLPESLKAARKRNEFPLFK 183 >At1g32140.1 68414.m03954 F-box family protein contains F-box domain Pfam:PF00646 Length = 591 Score = 26.2 bits (55), Expect = 4.5 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = -3 Query: 171 NSFELRTILFCPGVILCVNQSSS 103 N E+ + +C G++LCV ++ S Sbjct: 94 NQVEISKVFYCEGLLLCVTRNHS 116 >At5g46570.1 68418.m05734 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 489 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 2/43 (4%) Frame = -3 Query: 270 MHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSEN--SFELRTI 148 +HSK +++ + PS+ N SVNG + + +N FEL + Sbjct: 4 LHSKTANLPSSDDPSAPNKPESVNGDQVDQEIQNFKEFELNEL 46 >At5g44670.1 68418.m05473 expressed protein contains Pfam:PF01697 Domain of unknown function Length = 519 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/65 (21%), Positives = 30/65 (46%), Gaps = 1/65 (1%) Frame = +2 Query: 89 WHKFYELDWFTHKITPGQ-NKIVRNSNEFSLFKEDSLPLTDLMKLLDEGKVPLYMSEGFE 265 W F+++D F + + ++ + E+S F + +P++ + +G Y GFE Sbjct: 351 WMFFFDVDEFIYVPAKSSISSVMVSLEEYSQFTIEQMPMSSQLCYDGDGPARTYRKWGFE 410 Query: 266 CMPNR 280 + R Sbjct: 411 KLAYR 415 >At5g06580.1 68418.m00743 FAD linked oxidase family protein similar to SP|Q12627 from Kluyveromyces lactis and SP|P32891 from Saccharomyces cerevisiae; contains Pfam FAD linked oxidases, C-terminal domain PF02913, Pfam FAD binding domain PF01565 Length = 567 Score = 25.8 bits (54), Expect = 5.9 Identities = 24/98 (24%), Positives = 43/98 (43%), Gaps = 2/98 (2%) Frame = +2 Query: 11 ATDAVIK--IFLGPKYNDNGFPITLEENWHKFYELDWFTHKITPGQNKIVRNSNEFSLFK 184 +T+AV+K PK + LE+N Y+ +F K +K V N + +F Sbjct: 94 STEAVVKGEYKQVPKELISQLKTILEDNLTTDYDERYFHGKPQNSFHKAV-NIPDVVVFP 152 Query: 185 EDSLPLTDLMKLLDEGKVPLYMSEGFECMPNRLMLPRG 298 ++ ++K +E KVP+ G + + P+G Sbjct: 153 RSEEEVSKILKSCNEYKVPIVPYGGATSIEGHTLAPKG 190 >At3g16250.1 68416.m02050 ferredoxin-related contains Pfam profile: PF00111 2Fe-2S iron-sulfur cluster binding domains Length = 204 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = -3 Query: 288 NINLFGMHSKPSDIYNGTFPSSNNFMRSVNGKE 190 NI L+G +SKP G + + VNGKE Sbjct: 106 NIELYGPYSKPLSNCAGVGTCATCMVEIVNGKE 138 >At1g33610.1 68414.m04160 leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam:PF00560, INTERPRO:IPR001611 Length = 907 Score = 25.8 bits (54), Expect = 5.9 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 2/53 (3%) Frame = -3 Query: 300 VPLGNINLFGMHSKP--SDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRTI 148 VP+ NL + + ++ G FP ++S+NG ES S N F L+TI Sbjct: 716 VPMSFTNLINITNLDLSHNLLTGPFP----VLKSINGIESLDLSYNKFHLKTI 764 >At1g29355.1 68414.m03589 expressed protein Length = 307 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -3 Query: 249 IYNGTFPSSNNFMRSVNGKESSLKSENSFE 160 I+ G PS+N +N E +KSE ++E Sbjct: 265 IHIGELPSANPENTEINASEMEIKSEETWE 294 >At5g48620.1 68418.m06013 disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Length = 908 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = +1 Query: 85 KLA*ILRTG-LVHAQDNSWAKQDCTQLKRIFAFQRG 189 KL +L TG + D+ W K+D ++K +F +RG Sbjct: 258 KLFQLLETGRYLLVLDDVWKKEDWDRIKAVFPRKRG 293 >At4g16560.1 68417.m02505 heat shock protein-related contains similarity to SWISS-PROT:P31170 small heat shock protein, chloroplast precursor [Arabidopsis thaliana] Length = 532 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Frame = -1 Query: 308 HLQYLLATSIYLAC--IQNLQTYTTELFLRLITS*DRLTVKSPL 183 H +Y AT + C I N+Q + + +RLI S ++ ++ P+ Sbjct: 84 HRKYGTATGLICDCCEISNIQCFVGDGVVRLILSKQKINLRVPI 127 >At4g16550.1 68417.m02504 heat shock protein-related contains similarity to Swiss-Prot:P31170 small heat shock protein, chloroplast precursor [Arabidopsis thaliana] Length = 743 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Frame = -1 Query: 308 HLQYLLATSIYLAC--IQNLQTYTTELFLRLITS*DRLTVKSPL 183 H +Y AT + C I N+Q + + +RLI S ++ ++ P+ Sbjct: 238 HRKYGTATGLICDCCEISNIQCFVGDGVVRLILSKQKINLRVPI 281 >At3g63140.1 68416.m07091 mRNA-binding protein, putative similar to mRNA binding protein precursor (GI:26453355) [Lycopersicon esculentum] Length = 406 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = -3 Query: 258 PSDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRT 151 P ++ + PSS++ S++ SS S +F LRT Sbjct: 25 PPSLHRFSLPSSSSSFSSLSSSSSSSSSLLTFSLRT 60 >At3g54550.1 68416.m06036 hypothetical protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 288 Score = 25.4 bits (53), Expect = 7.8 Identities = 16/50 (32%), Positives = 23/50 (46%) Frame = -3 Query: 288 NINLFGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRTILFC 139 NIN GM P F + ++++ G +SSL ENS E+ C Sbjct: 11 NINGVGMRQAPRSRLKRLFERPFS-LKNLAGVDSSLSRENSEEIEPSSVC 59 >At3g17540.1 68416.m02240 F-box family protein similar to hypothetical protein GB:AAD18122 from [Arabidopsis thaliana];contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box protein interaction domain Length = 396 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/58 (25%), Positives = 28/58 (48%) Frame = -3 Query: 276 FGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGVILCVNQSSS 103 FG++ D++ G S + + + K S+LK + I C G+ILC + ++ Sbjct: 64 FGVYKITDDLH-GILNSGDPSLEFTS-KLSNLKISEDLTITKIFHCDGLILCSTKENT 119 >At1g73680.1 68414.m08532 pathogen-responsive alpha-dioxygenase, putative similar to pathogen-inducible alpha-dioxygenase [Nicotiana attenuata] GI:12539609; contains Pfam profile PF03098: Animal haem peroxidase Length = 631 Score = 25.4 bits (53), Expect = 7.8 Identities = 18/61 (29%), Positives = 33/61 (54%), Gaps = 5/61 (8%) Frame = +2 Query: 47 KYNDNGFPITLEENWHKFYELDWFTHKITPGQNKIVRNSNEFSLFKED-----SLPLTDL 211 K ND+G P +L E + Y + H + P + I+R+ N ++ KE+ +P+T+L Sbjct: 360 KPNDHGVPYSLTEEFVSVYRM----HCLLP-ETLILRDMNSENVDKENPAIEREIPMTEL 414 Query: 212 M 214 + Sbjct: 415 I 415 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,069,463 Number of Sequences: 28952 Number of extensions: 139883 Number of successful extensions: 481 Number of sequences better than 10.0: 28 Number of HSP's better than 10.0 without gapping: 472 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 481 length of database: 12,070,560 effective HSP length: 70 effective length of database: 10,043,920 effective search space used: 321405440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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