BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_F13
(696 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 29 0.48
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.84
SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 28 1.1
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 4.5
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 5.9
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 5.9
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 25 7.8
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 29.5 bits (63), Expect = 0.48
Identities = 27/103 (26%), Positives = 50/103 (48%), Gaps = 6/103 (5%)
Frame = +1
Query: 340 VQGLLSYPEICAKLINPNQNGKRPHLRKVNDP-SKRFGTYAFRLPDDXGEGGFWVSYEDP 516
V L+SYP++ ++ + + +LR++N P + F +A P E GF+V D
Sbjct: 1815 VNSLISYPKMNIEVSQSSSDKLLLYLRRLNQPLNPDFYIFAPLFPKPQSE-GFFVLIIDS 1873
Query: 517 DT----AGNKASYVKTKNLGGVSI-MDLSMDDFRGLCTGDNIR 630
+T A +AS+ +N + + + +SM D C N++
Sbjct: 1874 ETQELFAIRRASFAGRRNDDSIRLSLRISM-DIPPTCRNRNVK 1915
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.84
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -3
Query: 157 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 77
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 577
Score = 28.3 bits (60), Expect = 1.1
Identities = 23/79 (29%), Positives = 39/79 (49%), Gaps = 1/79 (1%)
Frame = +2
Query: 410 LIFVRSTILANVLGPMPSAFLMXMVKVA-SGFLTKILTRPETKLVT*KQRTLAVCPSWTF 586
++FV S + + +PS+F M MV +A S L+ T+ K+V+ T+ W F
Sbjct: 131 VLFVNSGMWSASTSFLPSSFAMNMVTLALSAQLSPPSTKRTVKVVS--FITIGAVIGWPF 188
Query: 587 PWMIFVVFALEIISDPEGR 643
+ + F L + D +GR
Sbjct: 189 SAALSIPFILLELVDLKGR 207
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 26.2 bits (55), Expect = 4.5
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +2
Query: 215 PTNLSL-VSAPLDVRGSSIQTVKFPEFHRYT 304
PT L SA DVR +S + +FH+Y+
Sbjct: 51 PTELKARTSATFDVRSASTSPINASDFHKYS 81
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +1
Query: 133 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWK--LDSDSEIS 285
P PQ+ + +QN + + + G P K V+ + T DSDS++S
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSDSKLS 829
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -1
Query: 69 DNVDQVYDAWDVKVNGAIYVWQ 4
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 25.4 bits (53), Expect = 7.8
Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 5/63 (7%)
Frame = +1
Query: 175 DAAINYWIQNGAPTHKLVLGISTTGRTWKLDSDSEISGVPP-----IHADXGGEAGPYTK 339
+ A YWI+ T + +LG S + + + IS + P H+ G GP TK
Sbjct: 792 EKATTYWIKGTEFTVERLLGYSNEAQRF-VGGSICISRLAPQDYHRFHSPVNGCIGPITK 850
Query: 340 VQG 348
++G
Sbjct: 851 IEG 853
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,065,087
Number of Sequences: 5004
Number of extensions: 67381
Number of successful extensions: 190
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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