Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_F05
(546 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 111 4e-25
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 111 4e-25
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 110 7e-25
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 108 2e-24
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 108 3e-24
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 108 3e-24
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 92 2e-19
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 88 4e-18
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 88 4e-18
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90 protein
Length = 823
Score = 111 bits (266), Expect = 4e-25
Identities = 62/182 (34%), Positives = 95/182 (52%), Gaps = 1/182 (0%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG++ED +NR+RLAKLLRF ++ L +Y+ RMK +Q I+YI
Sbjct: 523 KLGIIEDAANRNRLAKLLRFETTKSDG----------KLTSLDQYIKRMKKSQKDIFYIT 572
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
GSS+ ++++SPF ERL+++GYEV++ T+ VDEY + L +Y+ KFQN+++E + +
Sbjct: 573 GSSKEQLEKSPFLERLIKKGYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKVGKD- 631
Query: 363 XXXXXXXXXXXXXXPLTR-WLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERLA 539
LT+ W G+ V FGW+ NMER+
Sbjct: 632 ---SKDKELKEAFKELTKWWKGNLASENVDDVKISNRLADTPCVVVTSKFGWSANMERIM 688
Query: 540 LS 545
S
Sbjct: 689 QS 690
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90 protein
Length = 823
Score = 111 bits (266), Expect = 4e-25
Identities = 62/182 (34%), Positives = 95/182 (52%), Gaps = 1/182 (0%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG++ED +NR+RLAKLLRF ++ L +Y+ RMK +Q I+YI
Sbjct: 523 KLGIIEDAANRNRLAKLLRFETTKSDG----------KLTSLDQYIKRMKKSQKDIFYIT 572
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
GSS+ ++++SPF ERL+++GYEV++ T+ VDEY + L +Y+ KFQN+++E + +
Sbjct: 573 GSSKEQLEKSPFLERLIKKGYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKVGKD- 631
Query: 363 XXXXXXXXXXXXXXPLTR-WLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERLA 539
LT+ W G+ V FGW+ NMER+
Sbjct: 632 ---SKDKELKEAFKELTKWWKGNLASENVDDVKISNRLADTPCVVVTSKFGWSANMERIM 688
Query: 540 LS 545
S
Sbjct: 689 QS 690
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 110 bits (264), Expect = 7e-25
Identities = 59/178 (33%), Positives = 89/178 (50%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG+ ED NR+++A+LLR+ D + L +YV RMK Q+ I+YI
Sbjct: 417 KLGIHEDSQNRTKIAELLRYHSTKSG----------DELTSLKDYVTRMKEGQNEIFYIT 466
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
G S+ V+ SPF E+L ++GYEVLY+ +A+DEY + L E++G K + +E L+E+
Sbjct: 467 GESKKAVENSPFLEKLKKKGYEVLYMVDAIDEYAIGQLKEFEGKKLVSATKEGLKLEETD 526
Query: 363 XXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L + + D LG V + +GWT NMER+
Sbjct: 527 DEKKKKEELKEKFEGLCKVIKDVLGDKVEKVIVSDRVVDSPCCLVTGEYGWTANMERI 584
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 108 bits (260), Expect = 2e-24
Identities = 63/179 (35%), Positives = 85/179 (47%), Gaps = 1/179 (0%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG+ ED NR ++A LLR+ D M +YV RMK Q I+YI
Sbjct: 423 KLGIHEDSQNRGKIADLLRYHSTKSG----------DEMTSFKDYVTRMKEGQKDIFYIT 472
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDL-DES 359
G S+ V+ SPF ERL +RGYEVLY+ +A+DEY + L EYDG K + +E L DE+
Sbjct: 473 GESKKAVENSPFLERLKKRGYEVLYMVDAIDEYAVGQLKEYDGKKLVSATKEGLKLEDET 532
Query: 360 XXXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L + + + LG V + +GWT NMER+
Sbjct: 533 EEEKKKREEKKKSFENLCKTIKEILGDKVEKVVVSDRIVDSPCCLVTGEYGWTANMERI 591
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 108 bits (259), Expect = 3e-24
Identities = 59/178 (33%), Positives = 88/178 (49%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG+ ED NR+++A+LLR+ D + L +YV RMK Q+ I+YI
Sbjct: 417 KLGIHEDSQNRTKIAELLRYHSTKSG----------DELTSLKDYVTRMKEGQNDIFYIT 466
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
G S+ V+ SPF E+L ++G EVLY+ +A+DEY + L E++G K + +E LDE+
Sbjct: 467 GESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEGKKLVSATKEGLKLDETE 526
Query: 363 XXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L + + D LG V + +GWT NMER+
Sbjct: 527 DEKKKKEELKEKFEGLCKVIKDVLGDKVEKVIVSDRVVDSPCCLVTGEYGWTANMERI 584
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 108 bits (259), Expect = 3e-24
Identities = 59/178 (33%), Positives = 88/178 (49%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG+ ED NR+++A+LLR+ D + L +YV RMK Q+ I+YI
Sbjct: 417 KLGIHEDSQNRTKIAELLRYHSTKSG----------DELTSLKDYVTRMKEGQNDIFYIT 466
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
G S+ V+ SPF E+L ++G EVLY+ +A+DEY + L E++G K + +E LDE+
Sbjct: 467 GESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEGKKLVSATKEGLKLDETE 526
Query: 363 XXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L + + D LG V + +GWT NMER+
Sbjct: 527 DEKKKKEELKEKFEGLCKVIKDVLGDKVEKVIVSDRVVDSPCCLVTGEYGWTANMERI 584
>At3g07770.1 68416.m00947 heat shock protein-related strong similarity
to heat-shock protein [Secale cereale] GI:556673;
contains Pfam profiles PF02518: ATPase, histidine
kinase-, DNA gyrase B-, and HSP90-like domain protein,
PF00183: Hsp90 protein
Length = 803
Score = 92.3 bits (219), Expect = 2e-19
Identities = 58/180 (32%), Positives = 77/180 (42%), Gaps = 2/180 (1%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG +ED N R+A LLRF + M+ L EYV MKP Q IY+IA
Sbjct: 512 KLGCIEDRENHKRIAPLLRFFSSQSE----------NDMISLDEYVENMKPEQKAIYFIA 561
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLD--E 356
S + +PF E+++ +G EVLYL E +DE + SL Y F +I++E DL
Sbjct: 562 SDSITSAKNAPFLEKMLEKGLEVLYLVEPIDEVAVQSLKAYKEKDFVDISKEDLDLGNMS 621
Query: 357 SXXXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
W+ +LG V FGW+ NMERL
Sbjct: 622 GDKNEEKEAAVKKEFGQTCDWIKKRLGDKVASVQISNRLSSSPCVLVSGKFGWSANMERL 681
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 87.8 bits (208), Expect = 4e-18
Identities = 54/178 (30%), Positives = 78/178 (43%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG +ED N R+ LLRF + + L +Y+ M NQ IYY+A
Sbjct: 486 KLGCIEDTGNHKRITPLLRFFSSKNE----------EELTSLDDYIENMGENQKAIYYLA 535
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
S + +PF E+L+++ EVLYL E +DE + +L Y KF +I++E DL+
Sbjct: 536 TDSLKSAKSAPFLEKLIQKDIEVLYLVEPIDEVAIQNLQTYKEKKFVDISKE--DLELGD 593
Query: 363 XXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L W+ +LG V + FGW+ NMERL
Sbjct: 594 EDEVKDREAKQEFNLLCDWIKQQLGDKVAKVQVSNRLSSSPCVLVSGKFGWSANMERL 651
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 87.8 bits (208), Expect = 4e-18
Identities = 54/178 (30%), Positives = 78/178 (43%)
Frame = +3
Query: 3 KLGVMEDPSNRSRLAKLLRFXXXXXXXXXXXXXXXPDRMVFLSEYVARMKPNQHHIYYIA 182
KLG +ED N R+ LLRF + + L +Y+ M NQ IYY+A
Sbjct: 489 KLGCIEDTGNHKRITPLLRFFSSKNE----------EELTSLDDYIENMGENQKAIYYLA 538
Query: 183 GSSRAEVQRSPFAERLVRRGYEVLYLTEAVDEYCLSSLPEYDGHKFQNIAEEIFDLDESX 362
S + +PF E+L+++ EVLYL E +DE + +L Y KF +I++E DL+
Sbjct: 539 TDSLKSAKSAPFLEKLIQKDIEVLYLVEPIDEVAIQNLQTYKEKKFVDISKE--DLELGD 596
Query: 363 XXXXXXXXXXXXXXPLTRWLGDKLGAWVTRCXXXXXXXXXXXXXXXXXFGWTGNMERL 536
L W+ +LG V + FGW+ NMERL
Sbjct: 597 EDEVKDREAKQEFNLLCDWIKQQLGDKVAKVQVSNRLSSSPCVLVSGKFGWSANMERL 654
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,943,484
Number of Sequences: 28952
Number of extensions: 64944
Number of successful extensions: 209
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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