Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_E09
(255 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40770.1 68415.m05030 SNF2 domain-containing protein / helica... 30 0.18
At2g45870.1 68415.m05705 expressed protein contains Pfam profile... 29 0.32
At3g52970.1 68416.m05839 cytochrome P450 family protein cytochro... 28 0.74
At4g04110.1 68417.m00581 Toll-Interleukin-Resistance (TIR) domai... 27 2.3
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 26 3.0
At3g56150.1 68416.m06241 eukaryotic translation initiation facto... 26 3.0
At2g30370.1 68415.m03696 allergen-related weak similarity to pol... 26 4.0
At1g68890.1 68414.m07884 menaquinone biosynthesis protein-relate... 26 4.0
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 25 5.2
At3g48830.1 68416.m05333 polynucleotide adenylyltransferase fami... 25 5.2
At2g32950.1 68415.m04039 COP1 regulatory protein photomorphogene... 25 5.2
At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma mem... 25 6.9
At3g48770.1 68416.m05326 hypothetical protein 25 6.9
At5g61150.2 68418.m07672 leo1-like family protein weak similarit... 25 9.2
At5g61150.1 68418.m07671 leo1-like family protein weak similarit... 25 9.2
At5g56560.1 68418.m07058 F-box family protein contains F-box dom... 25 9.2
At5g11430.1 68418.m01335 transcription elongation factor-related... 25 9.2
At4g34940.1 68417.m04953 armadillo/beta-catenin repeat family pr... 25 9.2
At4g13630.1 68417.m02121 expressed protein contains Pfam profile... 25 9.2
At3g03220.1 68416.m00318 expansin, putative (EXP13) similar to e... 25 9.2
At1g11300.1 68414.m01298 S-locus lectin protein kinase family pr... 25 9.2
At1g03055.2 68414.m00279 expressed protein 25 9.2
At1g03055.1 68414.m00278 expressed protein 25 9.2
>At2g40770.1 68415.m05030 SNF2 domain-containing protein / helicase
domain-containing protein / zinc finger (C3HC4 type RING
finger) family protein low similarity to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00628: PHD-finger, PF00097: Zinc finger, C3HC4
type (RING finger)
Length = 1648
Score = 30.3 bits (65), Expect = 0.18
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = +3
Query: 45 ARKLISTMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQ 188
AR++I T+KR + P+ + +++N L+KL A C Q
Sbjct: 719 AREVIETLKRDILKRGHTSSDNPLVTHAEAAKLLNSLLKLRQACCHPQ 766
>At2g45870.1 68415.m05705 expressed protein contains Pfam profile
PF05249: Uncharacterised protein family (UPF0187)
Length = 410
Score = 29.5 bits (63), Expect = 0.32
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +3
Query: 24 KANSNRMARKLISTMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQHLNHS 203
K++ + ++ KLIS +K S+ I +R + K+ L N + S S++HL H
Sbjct: 49 KSDDSPLSEKLISLLKAVPNWSDGIKERR-MQQKRSLYTHENWVRHRS----SLRHLRHV 103
Query: 204 TSTPSCPVRLTFTKPHF 254
+S+PS V L+ P F
Sbjct: 104 SSSPSSRVILSLIPPVF 120
>At3g52970.1 68416.m05839 cytochrome P450 family protein cytochrome
P450 76A2, eggplant, PIR:S38534
Length = 516
Score = 28.3 bits (60), Expect = 0.74
Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%)
Frame = +2
Query: 47 KKIDFHDEKAINFVGNYWQENADLYE----EEVTKDY 145
+K FH EKA G + +E ++ E +E TKDY
Sbjct: 242 RKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDY 278
>At4g04110.1 68417.m00581 Toll-Interleukin-Resistance (TIR)
domain-containing protein domain signature TIR exists,
suggestive of a disease resistance protein.
Length = 239
Score = 26.6 bits (56), Expect = 2.3
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +3
Query: 18 YRKANSNRMARKLISTMKRQLTLSETIGKRTPICMKKKLQRIIND-LMKLSL 170
++K NR ++ + + ++ T+G + IC + I ND L+KL+L
Sbjct: 155 FKKTCQNRTEDEINLRRRALIDVANTLGYHSTICKANMTKEITNDVLVKLNL 206
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 26.2 bits (55), Expect = 3.0
Identities = 22/71 (30%), Positives = 29/71 (40%)
Frame = -2
Query: 251 VRFGKSQAHWA*RCACRMV*VLHRAHGERQFHKIVDNPL*LLLHTDRRSLANSFRQS*LP 72
+RFG+ A R ++ L H + H+ V P LLL + F S LP
Sbjct: 112 IRFGRLNESAARRYFQQLASALSFCHRDGIAHRDV-KPQNLLLDKQGNLKVSDFGLSALP 170
Query: 71 FHRGNQFSCHT 39
HR N HT
Sbjct: 171 EHRSNNGLLHT 181
>At3g56150.1 68416.m06241 eukaryotic translation initiation factor 3
subunit 8 / eIF3 p110 / eIF3c / p105 (TIF3C1) nearly
identical to SP|O49160 Eukaryotic translation initiation
factor 3 subunit 8 (eIF3 p110) (eIF3c) (p105)
{Arabidopsis thaliana}
Length = 900
Score = 26.2 bits (55), Expect = 3.0
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = +2
Query: 47 KKIDFHDEKAINFVGNYWQENADLYEEEVTKDYQRSYEI 163
KK+ + KA+N + ++N LYE+++ K Y+ + E+
Sbjct: 131 KKMSTSNSKALNSMKQKLKKNNKLYEDDINK-YREAPEV 168
>At2g30370.1 68415.m03696 allergen-related weak similarity to pollen
major allergen 2 protein [Juniperus ashei]
gi|9955725|emb|CAC05582
Length = 230
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 88 RKLLARERRSV*RRSYKGLSTIL*NCRSPCARCS 189
RK+ R + + RR +GL + C S C RC+
Sbjct: 162 RKIGDRSKEAELRRILRGLGSSPPRCSSKCGRCT 195
>At1g68890.1 68414.m07884 menaquinone biosynthesis protein-related
similar to menaquinone biosynthesis protein menD
[SP|P17109][E. coli] ; Pfam HMM hit:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic
acid synthase / 2-oxoglutarate decarboxylase
Length = 894
Score = 25.8 bits (54), Expect = 4.0
Identities = 14/54 (25%), Positives = 24/54 (44%)
Frame = +2
Query: 50 KIDFHDEKAINFVGNYWQENADLYEEEVTKDYQRSYEIVARHVLGAAPKPFDKH 211
++D H+E +I V W E+ E+ Y++S V+ H + KH
Sbjct: 232 ELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHFCPNVEDHWFKH 285
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 25.4 bits (53), Expect = 5.2
Identities = 12/54 (22%), Positives = 25/54 (46%)
Frame = +2
Query: 20 QKGKFESYGKKIDFHDEKAINFVGNYWQENADLYEEEVTKDYQRSYEIVARHVL 181
++ KF+ + + + + N N N D+ +E+ D + SY+ H+L
Sbjct: 234 EEAKFDDDDNALSYREVQGNNQEENVQLSNDDVLGDEIPYDQEVSYQNSINHML 287
>At3g48830.1 68416.m05333 polynucleotide adenylyltransferase family
protein / RNA recognition motif (RRM)-containing protein
similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19)
{Escherichia coli O157:H7}; contains Pfam profiles
PF01743: polyA polymerase family protein, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 881
Score = 25.4 bits (53), Expect = 5.2
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +3
Query: 72 RQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQHLNHSTSTPS 218
R + E IGK+ PIC I ND++++S S Q+ +T T S
Sbjct: 126 RSFSRCEIIGKKFPICH----VHIGNDMIEVSSFSTSAQNSLRNTRTGS 170
>At2g32950.1 68415.m04039 COP1 regulatory protein photomorphogenesis
repressor; identical to COP1 regulatory protein/FUSCA
protein FUS1 GI:402685 SP:P43254
Length = 675
Score = 25.4 bits (53), Expect = 5.2
Identities = 12/42 (28%), Positives = 17/42 (40%)
Frame = +3
Query: 111 PICMKKKLQRIINDLMKLSLAMCSVQHLNHSTSTPSCPVRLT 236
PICM+ + MC + HL + + P C LT
Sbjct: 53 PICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLT 94
>At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA10)
identical to SP|Q9SZR1 Potential calcium-transporting
ATPase 10, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 10) {Arabidopsis thaliana};
similar to SP|Q9LF79 Calcium-transporting ATPase 8,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
8) {Arabidopsis thaliana}
Length = 1069
Score = 25.0 bits (52), Expect = 6.9
Identities = 17/65 (26%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
Frame = +3
Query: 48 RKLIS--TMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQHLNHSTSTPSC 221
RK+++ + R+L+ ET+G T IC K +N+ M + +Q ++ S+
Sbjct: 455 RKMMADKALVRRLSACETMGSATTICSDKTGTLTLNE-MTVVECYAGLQKMDSPDSSSKL 513
Query: 222 PVRLT 236
P T
Sbjct: 514 PSAFT 518
>At3g48770.1 68416.m05326 hypothetical protein
Length = 1899
Score = 25.0 bits (52), Expect = 6.9
Identities = 8/22 (36%), Positives = 12/22 (54%)
Frame = -3
Query: 211 VLVEWFRCCTEHMASDNFIRSL 146
+L+EW C H NF+ S+
Sbjct: 959 LLLEWINKCNRHSLRSNFLNSV 980
>At5g61150.2 68418.m07672 leo1-like family protein weak similarity
to SP|P38439 LEO1 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04004: Leo1-like protein;
supporting cDNA gi|21929714|gb|AF490422.1|
Length = 623
Score = 24.6 bits (51), Expect = 9.2
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 161 IVARHVLGAAPKPFDKHTFM 220
I +++G PKPFD TF+
Sbjct: 264 IKVSNIMGIDPKPFDAKTFV 283
>At5g61150.1 68418.m07671 leo1-like family protein weak similarity
to SP|P38439 LEO1 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04004: Leo1-like protein;
supporting cDNA gi|21929714|gb|AF490422.1|
Length = 625
Score = 24.6 bits (51), Expect = 9.2
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 161 IVARHVLGAAPKPFDKHTFM 220
I +++G PKPFD TF+
Sbjct: 264 IKVSNIMGIDPKPFDAKTFV 283
>At5g56560.1 68418.m07058 F-box family protein contains F-box domain
Pfam:PF00646
Length = 607
Score = 24.6 bits (51), Expect = 9.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +1
Query: 172 PCARCST*TIRQAHLHAQCA*LLP 243
PC CS T+++ LH C ++P
Sbjct: 122 PCCLCSCITLKELTLHDLCIKVVP 145
>At5g11430.1 68418.m01335 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 871
Score = 24.6 bits (51), Expect = 9.2
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +3
Query: 60 STMKRQLTLSETIGKRTPICMKKKLQRIINDLMKLSLAMCSVQHLNH 200
S KRQ + S +IG +T + K QR+ + + L + S+++ +H
Sbjct: 692 SGSKRQHSFSSSIGSKTSVLPVNKKQRV--HVTEKPLVVASMRNHHH 736
>At4g34940.1 68417.m04953 armadillo/beta-catenin repeat family
protein contains Pfam profile: PF00514
armadillo/beta-catenin-like repeat
Length = 664
Score = 24.6 bits (51), Expect = 9.2
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -3
Query: 187 CTEHMASDNFIRSLIILCNFFFIQIGVLLPIVSDK 83
C +H A +N IR L+ F +Q IVS+K
Sbjct: 263 CQDHFAQNNIIRFLVSHLAFETVQEHSKYAIVSNK 297
>At4g13630.1 68417.m02121 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 581
Score = 24.6 bits (51), Expect = 9.2
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 11 QSLQKGKFESYGKKIDFH 64
QSL+KG F+ K++ FH
Sbjct: 132 QSLKKGSFKVKSKRLSFH 149
>At3g03220.1 68416.m00318 expansin, putative (EXP13) similar to
expansin precursor GB:AAD13631 from [Lycopersicon
esculentum]; alpha-expansin gene family, PMID:11641069
Length = 266
Score = 24.6 bits (51), Expect = 9.2
Identities = 11/29 (37%), Positives = 20/29 (68%)
Frame = +3
Query: 132 LQRIINDLMKLSLAMCSVQHLNHSTSTPS 218
+QR + L+ L+L+ ++ H + STS+PS
Sbjct: 1 MQRFLLPLLFLALSPPAICHYSSSTSSPS 29
>At1g11300.1 68414.m01298 S-locus lectin protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains S-locus glycoprotein family domain,
Pfam:PF00954
Length = 1635
Score = 24.6 bits (51), Expect = 9.2
Identities = 13/50 (26%), Positives = 22/50 (44%)
Frame = -3
Query: 178 HMASDNFIRSLIILCNFFFIQIGVLLPIVSDKVNCLFIVEINFLAIRFEF 29
H +S F+ L++ C F + + S K+N + +F RF F
Sbjct: 4 HESSSPFVCILVLSCFFLSVSLAQERAFFSGKLNDSETIVSSFRTFRFGF 53
>At1g03055.2 68414.m00279 expressed protein
Length = 200
Score = 24.6 bits (51), Expect = 9.2
Identities = 16/59 (27%), Positives = 26/59 (44%)
Frame = +2
Query: 59 FHDEKAINFVGNYWQENADLYEEEVTKDYQRSYEIVARHVLGAAPKPFDKHTFMPSALD 235
F + AIN++ Q+ A + + DY R + R K +H F+ S+LD
Sbjct: 69 FFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTK--QQHEFVLSSLD 125
>At1g03055.1 68414.m00278 expressed protein
Length = 264
Score = 24.6 bits (51), Expect = 9.2
Identities = 16/59 (27%), Positives = 26/59 (44%)
Frame = +2
Query: 59 FHDEKAINFVGNYWQENADLYEEEVTKDYQRSYEIVARHVLGAAPKPFDKHTFMPSALD 235
F + AIN++ Q+ A + + DY R + R K +H F+ S+LD
Sbjct: 69 FFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTK--QQHEFVLSSLD 125
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,797,479
Number of Sequences: 28952
Number of extensions: 102494
Number of successful extensions: 342
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 338
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 342
length of database: 12,070,560
effective HSP length: 63
effective length of database: 10,246,584
effective search space used: 215178264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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