Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_E08
(515 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g19800.1 68417.m02904 glycosyl hydrolase family 18 protein si... 32 0.20
At3g16460.2 68416.m02097 jacalin lectin family protein contains ... 31 0.61
At3g16460.1 68416.m02098 jacalin lectin family protein contains ... 31 0.61
At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family pr... 30 0.80
At4g34350.1 68417.m04881 LytB family protein contains Pfam profi... 28 3.2
At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein si... 28 3.2
At4g20190.1 68417.m02952 hypothetical protein 27 5.7
At3g11670.2 68416.m01431 digalactosyldiacylglycerol synthase 1 (... 27 5.7
At3g11670.1 68416.m01430 digalactosyldiacylglycerol synthase 1 (... 27 5.7
At2g46530.2 68415.m05803 transcriptional factor B3 family protei... 27 5.7
At2g46530.1 68415.m05802 transcriptional factor B3 family protei... 27 5.7
At5g65630.1 68418.m08256 DNA-binding bromodomain-containing prot... 27 7.5
At5g57000.1 68418.m07114 expressed protein similar to unknown pr... 27 7.5
At4g25390.2 68417.m03653 protein kinase family protein contains ... 27 7.5
At4g25390.1 68417.m03652 protein kinase family protein contains ... 27 7.5
At4g19770.1 68417.m02901 glycosyl hydrolase family 18 protein si... 27 7.5
At4g19730.1 68417.m02897 glycosyl hydrolase family 18 protein si... 27 7.5
At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containi... 27 7.5
At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE... 27 9.9
At4g27690.1 68417.m03981 vacuolar protein sorting-associated pro... 27 9.9
At3g50670.1 68416.m05542 U1 small nuclear ribonucleoprotein 70 (... 27 9.9
At3g07300.1 68416.m00869 eukaryotic translation initiation facto... 27 9.9
At1g07650.1 68414.m00821 leucine-rich repeat transmembrane prote... 27 9.9
>At4g19800.1 68417.m02904 glycosyl hydrolase family 18 protein
similar to chitinase, class V GI:899342 from [Nicotiana
tabacum]
Length = 398
Score = 32.3 bits (70), Expect = 0.20
Identities = 36/142 (25%), Positives = 53/142 (37%)
Frame = +1
Query: 1 GAPTHKLVLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKLI 180
G P K VLG G W L +D +++G GP G +SY ++ ++
Sbjct: 224 GLPAKKAVLGFPYYGWAWTL-ADPDVNGYD------ANTTGPAISDDGEISYRQLQTWIV 276
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNKASYVKTKNLG 360
+ NG KV+D G Y + G W+ Y+ + K Y K K L
Sbjct: 277 D---NGAT----KVHD-DMMVGDYCYA-------GTTWIGYDSEKSIVTKVIYAKQKGLL 321
Query: 361 GVSIMDLSMDDFRGLCTGDKYP 426
G + DD L + P
Sbjct: 322 GYFSWHVGGDDNSELSSAGSTP 343
>At3g16460.2 68416.m02097 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 647
Score = 30.7 bits (66), Expect = 0.61
Identities = 24/80 (30%), Positives = 33/80 (41%), Gaps = 1/80 (1%)
Frame = +1
Query: 1 GAPTHKL-VLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKL 177
G+ KL G ST G +W D S+ GV I+A GGE Y K + L
Sbjct: 249 GSGAQKLEAQGNSTGGTSW--DDGSDYDGVTKIYASYGGEGIQYVKFDYVKGGVTKQGVL 306
Query: 178 INPNQNGKRPHLRKVNDPSK 237
Q+ + P +N P +
Sbjct: 307 HGKQQSRQNPREFVINHPDE 326
>At3g16460.1 68416.m02098 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 705
Score = 30.7 bits (66), Expect = 0.61
Identities = 24/80 (30%), Positives = 33/80 (41%), Gaps = 1/80 (1%)
Frame = +1
Query: 1 GAPTHKL-VLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKL 177
G+ KL G ST G +W D S+ GV I+A GGE Y K + L
Sbjct: 249 GSGAQKLEAQGNSTGGTSW--DDGSDYDGVTKIYASYGGEGIQYVKFDYVKGGVTKQGVL 306
Query: 178 INPNQNGKRPHLRKVNDPSK 237
Q+ + P +N P +
Sbjct: 307 HGKQQSRQNPREFVINHPDE 326
>At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family
protein bHLH protein, Arabidopsis thaliana,
PATCHX:E255557
Length = 589
Score = 30.3 bits (65), Expect = 0.80
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = +1
Query: 223 NDPSKRFGTYAFRLPDDHGEG--GFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDDF 396
N+ FG++AF L D GE G W+S + +G A+ V N G S +
Sbjct: 253 NNGGGEFGSWAFNLNPDQGENDPGLWISEPNGVDSGLVAAPV-MNNGGNDSTSNSDSQPI 311
Query: 397 RGLCTG 414
LC G
Sbjct: 312 SKLCNG 317
>At4g34350.1 68417.m04881 LytB family protein contains Pfam profile:
PF02401 LytB protein
Length = 466
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = +1
Query: 19 LVLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAK 174
LV+G + T L SE G+P D GP K+ L Y E+ K
Sbjct: 372 LVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGELVEK 423
>At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein
similar to chitinase/lysozyme GI:467689 from [Nicotiana
tabacum]
Length = 379
Score = 28.3 bits (60), Expect = 3.2
Identities = 34/135 (25%), Positives = 48/135 (35%)
Frame = +1
Query: 1 GAPTHKLVLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKLI 180
G P K VLG G W+L + + S P G P G + Y +I ++
Sbjct: 246 GLPAKKAVLGFPYYGYAWRLTNANSHSYYAPT---TGAAISP----DGSIGYGQIRKFIV 298
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNKASYVKTKNLG 360
+ NG S G Y + G W+ Y+D + K Y K + L
Sbjct: 299 D---NGATTVYN-----STVVGDYCYA-------GTNWIGYDDNQSIVTKVRYAKQRGLL 343
Query: 361 GVSIMDLSMDDFRGL 405
G + DD GL
Sbjct: 344 GYFSWHVGADDNSGL 358
>At4g20190.1 68417.m02952 hypothetical protein
Length = 389
Score = 27.5 bits (58), Expect = 5.7
Identities = 19/88 (21%), Positives = 34/88 (38%)
Frame = -3
Query: 414 SSAKTTKIIHGKVHDGHTAKVLCFHVTSFVSGRVRIFVRNPEATFTMIIRKAEGIGPKTF 235
S+ +K I+ DG LC ++ F G+ R +++FT R ++
Sbjct: 195 SNTANSKSINS-FEDGFKCSALCLYLPGFSKGKPVRSSRKGDSSFT---RTTTMTSSQSM 250
Query: 234 ARIVDLTKMRTLSVLVGVNKFGANFWVA 151
AR + LS + +F W +
Sbjct: 251 ARTASIRDTAVLSARASLERFECGSWTS 278
>At3g11670.2 68416.m01431 digalactosyldiacylglycerol synthase 1
(DGD1) / MGDG:MGDG galactosyltransferase / galactolipid
galactosyltransferase identical to
digalactosyldiacylglycerol synthase (DGD1) GI:5354158
[Arabidopsis thaliana]
Length = 639
Score = 27.5 bits (58), Expect = 5.7
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +1
Query: 274 HGEGGFWVSYEDPDTAGNK 330
H +GGFW + P+T NK
Sbjct: 325 HYDGGFWTDFVKPETPENK 343
>At3g11670.1 68416.m01430 digalactosyldiacylglycerol synthase 1
(DGD1) / MGDG:MGDG galactosyltransferase / galactolipid
galactosyltransferase identical to
digalactosyldiacylglycerol synthase (DGD1) GI:5354158
[Arabidopsis thaliana]
Length = 808
Score = 27.5 bits (58), Expect = 5.7
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +1
Query: 274 HGEGGFWVSYEDPDTAGNK 330
H +GGFW + P+T NK
Sbjct: 325 HYDGGFWTDFVKPETPENK 343
>At2g46530.2 68415.m05803 transcriptional factor B3 family protein /
auxin-responsive factor AUX/IAA-related contains Pfam
profiles: PF02309 AUX/IAA family, PF02362 B3 DNA binding
domain
Length = 514
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +2
Query: 158 QKFAPNLLTPTKTESVLIFVRSTILANVLG-PMPSAFL 268
+ F+P+ LTPT T+ RS ++ + G P+ S+FL
Sbjct: 263 EPFSPSALTPTPTQQQSKSKRSRPISEITGSPVASSFL 300
>At2g46530.1 68415.m05802 transcriptional factor B3 family protein /
auxin-responsive factor AUX/IAA-related contains Pfam
profiles: PF02309 AUX/IAA family, PF02362 B3 DNA binding
domain
Length = 601
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +2
Query: 158 QKFAPNLLTPTKTESVLIFVRSTILANVLG-PMPSAFL 268
+ F+P+ LTPT T+ RS ++ + G P+ S+FL
Sbjct: 350 EPFSPSALTPTPTQQQSKSKRSRPISEITGSPVASSFL 387
>At5g65630.1 68418.m08256 DNA-binding bromodomain-containing protein
similar to 5.9 kb fsh membrane protein [Drosophila
melanogaster] GI:157455; contains Pfam profile PF00439:
Bromodomain
Length = 590
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYA 255
NPN N +R + K D S FG+YA
Sbjct: 44 NPNPNFERSNSSKQCDDSSEFGSYA 68
>At5g57000.1 68418.m07114 expressed protein similar to unknown
protein (gb|AAF21159.1)
Length = 187
Score = 27.1 bits (57), Expect = 7.5
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -2
Query: 307 LRKKPRSHLHHDHQE 263
L++KP HLHH H+E
Sbjct: 87 LKEKPPRHLHHHHKE 101
>At4g25390.2 68417.m03653 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 497
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNKASYVKTKNLG 360
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 361 GV 366
+
Sbjct: 133 SL 134
>At4g25390.1 68417.m03652 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 651
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNKASYVKTKNLG 360
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 361 GV 366
+
Sbjct: 133 SL 134
>At4g19770.1 68417.m02901 glycosyl hydrolase family 18 protein
similar to chitinase, class V GI:899342 from [Nicotiana
tabacum]
Length = 261
Score = 27.1 bits (57), Expect = 7.5
Identities = 33/142 (23%), Positives = 48/142 (33%)
Frame = +1
Query: 1 GAPTHKLVLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKLI 180
G P K VLG G W L P H GP G +SY ++ ++
Sbjct: 137 GLPAEKAVLGFPYYGWAWTLAD-------PKNHGYYVDTTGPAISDDGEISYSQLKTWIV 189
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNKASYVKTKNLG 360
+ V+D + G Y + G W+ Y+ ++ K Y K K L
Sbjct: 190 DNKAT-------TVHD-NIVIGDYCYA-------GTTWIGYDSEESIVTKVIYAKQKGLL 234
Query: 361 GVSIMDLSMDDFRGLCTGDKYP 426
G + DD L + P
Sbjct: 235 GYFSWQVGGDDKSELSSAGSSP 256
>At4g19730.1 68417.m02897 glycosyl hydrolase family 18 protein
similar to chitinase, class V GI:505267 from [Nicotiana
tabacum]
Length = 332
Score = 27.1 bits (57), Expect = 7.5
Identities = 30/110 (27%), Positives = 42/110 (38%)
Frame = +1
Query: 1 GAPTHKLVLGISTTGRTWKLDSDSEISGVPPIHADKGGEAGPYTKVQGLLSYPEICAKLI 180
G P K VLG S G W L +D + +G A ++ G ++Y +I
Sbjct: 239 GLPEKKAVLGFSYVGWAWTLQNDKD-TGYNAAAAGVAKSEDDVSE-DGSINYAQI----- 291
Query: 181 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDHGEGGFWVSYEDPDTAGNK 330
N+ + KV DP K G Y F W+ YED + K
Sbjct: 292 --NKFIRDEEAAKVYDP-KVVGHYCFAKK-------IWIGYEDTQSVEAK 331
>At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 501
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = -3
Query: 258 EGIGPKTFARIVDLTKMRTLSVLVGVNKFG-ANFW 157
EGIG K R+++L R+ ++++ N+F A W
Sbjct: 401 EGIGEKVKKRLIELEPKRSGNLVIVANRFAEARMW 435
>At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE) /
sigma-like factor (SIG5) identical to RNA polymerase
sigma subunit SigE [Arabidopsis thaliana] GI:4972299,
sigma-like factor [Arabidopsis thaliana] GI:4033838;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2
Length = 517
Score = 26.6 bits (56), Expect = 9.9
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +1
Query: 361 GVSIMDLSMDDFRGLCTG-DKYPILRAAKYRL*TFSLYF*KHAL 489
G DL RGL T D++ R K+RL T+ L++ +HA+
Sbjct: 305 GPKFQDLCQAGMRGLITAIDRFEPKR--KFRLSTYGLFWIRHAI 346
>At4g27690.1 68417.m03981 vacuolar protein sorting-associated
protein 26, putative / VPS26, putative similar to
vacuolar sorting protein 26 [Homo sapiens] GI:9622852;
contains Pfam profile PF03643: Vacuolar protein
sorting-associated protein 26
Length = 303
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 423 IFISSAKTTKIIHGKVHDGHTAKVLCFHVTSFVSGRVRI 307
I S K K + K +G TA V FH +SG+V I
Sbjct: 16 ITFSDGKNRKQVPMKKENGQTALVPLFHSQDTISGKVCI 54
>At3g50670.1 68416.m05542 U1 small nuclear ribonucleoprotein 70
(U1-70k)
Length = 427
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = -2
Query: 322 RPCQDLRKKPRSHLHH-DHQEGGRHRSQNVC*DR 224
+P + R +PR HH D +GGR R ++ DR
Sbjct: 285 QPRERSRDRPREDKHHRDRDQGGRDRDRDSRRDR 318
>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P49770 Translation initiation factor eIF-2B beta
subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF01008: Initiation factor 2
subunit family
Length = 407
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 346 TKNLGGVSIMDLSMDDFRGLCTGDKYPILRAA 441
T +GG+ ++D S DD C G +P + AA
Sbjct: 93 TAAMGGLDLLDASDDDDVDNCKGIGFPAMSAA 124
>At1g07650.1 68414.m00821 leucine-rich repeat transmembrane protein
kinase, putative similar to GB:AAC50043 from
[Arabidopsis thaliana] (Plant Mol. Biol. 37 (4), 587-596
(1998))
Length = 1014
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = -3
Query: 303 VRNPEATFTMIIRKAEGIGPKTFARIVDLTKMRTLSV 193
++N E+ T+I+RK + IGP I DL K++TL +
Sbjct: 277 LKNLESIKTLILRKCKIIGPIP-KYIGDLKKLKTLDL 312
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,601,899
Number of Sequences: 28952
Number of extensions: 289909
Number of successful extensions: 780
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 779
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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