Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_E02
(629 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 73 2e-13
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 68 4e-12
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 67 8e-12
At1g64030.1 68414.m07252 serpin family protein / serine protease... 62 4e-10
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 58 5e-09
At2g35580.1 68415.m04357 serpin family protein / serine protease... 50 1e-06
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 42 3e-04
At1g62170.1 68414.m07013 serpin family protein / serine protease... 42 3e-04
At2g14540.1 68415.m01628 serpin family protein / serine protease... 40 0.001
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 39 0.002
At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 3.4
At1g70900.1 68414.m08181 expressed protein 29 3.4
At1g23110.1 68414.m02889 hypothetical protein 29 3.4
At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 5.9
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 5.9
At4g01290.1 68417.m00170 expressed protein 28 5.9
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 5.9
At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 7.8
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 7.8
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 72.5 bits (170), Expect = 2e-13
Identities = 54/185 (29%), Positives = 90/185 (48%), Gaps = 9/185 (4%)
Frame = +2
Query: 23 TSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKF--LGNGDY-SGVANPYISLSKTF 193
T + N+V SP+ + +L+ L AG+ ++ +I F L + DY + V +S++
Sbjct: 24 TVANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALND 83
Query: 194 SEMNPD-YFTMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI--INQWA 361
D + + A +++ + F + Y + +DF+ TK A I +N WA
Sbjct: 84 GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFA-TKPAEVINEVNAWA 142
Query: 362 NEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTTIVK 535
T G IK +SDD+I + L N ++F+G W F+A TK DFH+ T+VK
Sbjct: 143 EVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHLLDGTMVK 202
Query: 536 KPTMS 550
P M+
Sbjct: 203 VPFMT 207
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 68.1 bits (159), Expect = 4e-12
Identities = 47/176 (26%), Positives = 81/176 (46%), Gaps = 7/176 (3%)
Frame = +2
Query: 41 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 220
NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + +
Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90
Query: 221 MANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTK-KAADIINQWANEKTRGHIKSP 394
A+ +++ L F + Y++ +DF+ + D +N WA+ T G IK
Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFATKPVEVIDEVNIWADVHTNGLIKQI 150
Query: 395 ISDDTIDP-----NAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTTIVKKPTM 547
+S D D N+ L N ++F+ W F+A TK+ DFH+ VK P M
Sbjct: 151 LSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDNDFHLLDGNTVKVPFM 206
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 67.3 bits (157), Expect = 8e-12
Identities = 37/115 (32%), Positives = 62/115 (53%), Gaps = 4/115 (3%)
Frame = +2
Query: 218 TMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDF-SDTKKAADIINQWANEKTRGHIKS 391
++AN +++ ++L F + Y++ +DF S + D +N WA T G IK
Sbjct: 28 SIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVHTNGLIKQ 87
Query: 392 PISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTTIVKKPTMS 550
+S D+ID ++ L N ++F+G W F+A+ TK+ DFH+ T VK P M+
Sbjct: 88 ILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLDGTSVKVPFMT 142
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 61.7 bits (143), Expect = 4e-10
Identities = 44/183 (24%), Positives = 76/183 (41%), Gaps = 7/183 (3%)
Frame = +2
Query: 23 TSSKDKNVVSSPLGVMMLMLLYKAG-AGEGSRAEIDKFLGNGDYSGVANPYISLSKTF-- 193
++ KD NV+ SP + + ++ AG G+ +I FL + + + L+
Sbjct: 24 SAPKDSNVIFSPASINSAITMHAAGPGGDLVSGQILSFLRSSSIDELKTVFRELASVVYA 83
Query: 194 --SEMNPDYFTMANKIYVGNKYTLDEKFTITVRQYQSEVET-IDF-SDTKKAADIINQWA 361
S T AN +++ D KF + V +DF S+ ++ +N W
Sbjct: 84 DRSATGGPKITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWV 143
Query: 362 NEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTTIVKKP 541
T IK + D ++ N + F+G W PF T++ DF++ + T V P
Sbjct: 144 EHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVP 203
Query: 542 TMS 550
MS
Sbjct: 204 FMS 206
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 58.0 bits (134), Expect = 5e-09
Identities = 42/184 (22%), Positives = 82/184 (44%), Gaps = 8/184 (4%)
Frame = +2
Query: 23 TSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFL---GNGDYSGVANPYISLSKTF 193
T S++ NV+ SP + +++ + AG+ ++ +I FL + ++ +S
Sbjct: 24 TVSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFLKFSSTDQLNSFSSEIVSAVLAD 83
Query: 194 SEMNPD-YFTMANKIYVGNKYTLDEKFTITVRQ-YQSEVETIDFSDTKKAADII---NQW 358
N ++AN ++ + F + Y++ DF KA ++I N W
Sbjct: 84 GSANGGPKLSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS--KAVEVIAEVNSW 141
Query: 359 ANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTTIVKK 538
A ++T G I + + + D N ++F+G W+ F+ S T+E +FH+ V
Sbjct: 142 AEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGNKVTA 201
Query: 539 PTMS 550
P M+
Sbjct: 202 PFMT 205
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 50.0 bits (114), Expect = 1e-06
Identities = 30/114 (26%), Positives = 55/114 (48%), Gaps = 5/114 (4%)
Frame = +2
Query: 224 ANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI---INQWANEKTRGHIKS 391
AN +++ ++ F + + Y++ +DF KA ++ +N W ++T G I +
Sbjct: 95 ANGLWIEKTLNVEPSFKDLLLNSYKAAFNRVDFRT--KADEVNREVNSWVEKQTNGLITN 152
Query: 392 PISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETKEKDFHVDSTTIVKKPTMS 550
+ + +F N +FF G W F+ S TK+ DFH+ T V+ P M+
Sbjct: 153 LLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDGTKVRVPFMT 206
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 41.9 bits (94), Expect = 3e-04
Identities = 16/55 (29%), Positives = 29/55 (52%)
Frame = +2
Query: 347 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFH 511
+N+WA++ T G I + ++ N ++F+G W F+ S TK+ +FH
Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFH 70
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 41.9 bits (94), Expect = 3e-04
Identities = 23/81 (28%), Positives = 40/81 (49%), Gaps = 1/81 (1%)
Frame = +2
Query: 311 IDF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNAS 487
+DF S ++ +N WA+ T G IK + ++ + ++F+G W ++ S
Sbjct: 190 VDFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKS 249
Query: 488 ETKEKDFHVDSTTIVKKPTMS 550
TK K F++ + T V P MS
Sbjct: 250 MTKCKPFYLLNGTSVSVPFMS 270
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 40.3 bits (90), Expect = 0.001
Identities = 22/94 (23%), Positives = 42/94 (44%), Gaps = 1/94 (1%)
Frame = +2
Query: 290 YQSEVETIDFSDTKKAADI-INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHW 466
+++ +DF + + +N WA+ T IK + ++ N ++F+G W
Sbjct: 147 FKASFAKVDFRHKAEEVRLDVNTWASRHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAW 206
Query: 467 HVPFNASETKEKDFHVDSTTIVKKPTMSLLQPLF 568
F+ S T++K FH+ + V P M + F
Sbjct: 207 EKAFDKSMTRDKPFHLLNGKSVSVPFMRSYEKQF 240
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 39.1 bits (87), Expect = 0.002
Identities = 20/67 (29%), Positives = 30/67 (44%)
Frame = +2
Query: 347 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 526
+N WA T G IK+ + ++ N ++F+G W F S T K FH+ +
Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98
Query: 527 IVKKPTM 547
V P M
Sbjct: 99 QVLVPFM 105
>At3g02280.1 68416.m00209 flavodoxin family protein low similarity
to SP|Q05001 NADPH-cytochrome P450 reductase (EC
1.6.2.4) {Catharanthus roseus}, similar to
NADPH-dependent FMN and FAD containing oxidoreductase
[Homo sapiens] GI:6694369; contains Pfam profiles
PF00258: flavodoxin, PF00667: FAD binding domain,
PF00175: Oxidoreductase NAD-binding domain
Length = 623
Score = 28.7 bits (61), Expect = 3.4
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%)
Frame = +2
Query: 107 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 268
G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K
Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182
Query: 269 FTITV-RQYQSEVETIDFSD 325
+ I +Q + E + + SD
Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202
>At1g70900.1 68414.m08181 expressed protein
Length = 244
Score = 28.7 bits (61), Expect = 3.4
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +2
Query: 38 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 217
K +S +GV + LY + SR + K+L DY+ +A I L++ E NP +
Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157
Query: 218 TMANKI 235
A+ +
Sbjct: 158 MAASAL 163
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 28.7 bits (61), Expect = 3.4
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +2
Query: 38 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 217
K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP +
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 218 TMANKI 235
A+ +
Sbjct: 175 MAASAL 180
>At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK16) mitogen-activated protein
kinase (MAPK)(AtMPK16), PMID:12119167; similar to
ATMPK9, Arabidopsis thaliana, EMBL:AB038694
Length = 567
Score = 27.9 bits (59), Expect = 5.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 101 GEGSRAEIDKFLGNGDYSGVANPY 172
GEGSR I++ +G G Y V + Y
Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 27.9 bits (59), Expect = 5.9
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -3
Query: 90 LYRSISIITPNGEDTTFLSFDDVYVSLRN 4
LYR+ +++ P G+D ++S D+ RN
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 27.9 bits (59), Expect = 5.9
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%)
Frame = +3
Query: 225 PIKSTSEINILWMRNSRSLSVSTKAKLKLLT----SAIPKKQLTSLISGLMRRP 374
P+ + + + M R SV +A LL+ S+ PK Q T L+S RRP
Sbjct: 584 PVDQDTSVPSVKMTKQRKTSVDDQASQHLLSLLQRSSDPKSQDTQLLSATERRP 637
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 27.9 bits (59), Expect = 5.9
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +2
Query: 41 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 220
+VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337
Query: 221 MANKIYVGNKYTLDEKF 271
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 444
Score = 27.5 bits (58), Expect = 7.8
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +2
Query: 35 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 211
DKNV S LG + +L +G + + GDY G+ Y++ T M D
Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188
Query: 212 Y 214
Y
Sbjct: 189 Y 189
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 27.5 bits (58), Expect = 7.8
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +2
Query: 125 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 247
D++L Y P I KT S P+ N++Y+GN
Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,004,396
Number of Sequences: 28952
Number of extensions: 298024
Number of successful extensions: 938
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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