Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_D17
(500 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family pr... 30 0.76
At5g57000.1 68418.m07114 expressed protein similar to unknown pr... 28 3.1
At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein si... 28 4.1
At1g79890.1 68414.m09334 helicase-related similar to CHL1 potent... 28 4.1
At5g65630.1 68418.m08256 DNA-binding bromodomain-containing prot... 27 7.1
At4g25390.2 68417.m03653 protein kinase family protein contains ... 27 7.1
At4g25390.1 68417.m03652 protein kinase family protein contains ... 27 7.1
At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE... 27 9.4
At4g27690.1 68417.m03981 vacuolar protein sorting-associated pro... 27 9.4
At3g07300.1 68416.m00869 eukaryotic translation initiation facto... 27 9.4
At1g07650.1 68414.m00821 leucine-rich repeat transmembrane prote... 27 9.4
>At4g17880.1 68417.m02665 basic helix-loop-helix (bHLH) family
protein bHLH protein, Arabidopsis thaliana,
PATCHX:E255557
Length = 589
Score = 30.3 bits (65), Expect = 0.76
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = +1
Query: 55 NDPSKRFGTYAFRLPDDNGEG--GFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDDF 228
N+ FG++AF L D GE G W+S + +G A+ V N G S +
Sbjct: 253 NNGGGEFGSWAFNLNPDQGENDPGLWISEPNGVDSGLVAAPV-MNNGGNDSTSNSDSQPI 311
Query: 229 RGLCTG 246
LC G
Sbjct: 312 SKLCNG 317
>At5g57000.1 68418.m07114 expressed protein similar to unknown
protein (gb|AAF21159.1)
Length = 187
Score = 28.3 bits (60), Expect = 3.1
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = -2
Query: 139 LRKKPRSHLHHYHQE 95
L++KP HLHH+H+E
Sbjct: 87 LKEKPPRHLHHHHKE 101
>At4g19810.1 68417.m02905 glycosyl hydrolase family 18 protein
similar to chitinase/lysozyme GI:467689 from [Nicotiana
tabacum]
Length = 379
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +1
Query: 115 GGFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDDFRGL 237
G W+ Y+D + K Y K + L G + DD GL
Sbjct: 318 GTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSGL 358
>At1g79890.1 68414.m09334 helicase-related similar to CHL1 potential
helicase protein (GI:2632247) [Homo sapiens]; similar to
helicase GB:AAB06962 [Homo sapiens]
Length = 882
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/53 (24%), Positives = 23/53 (43%)
Frame = +1
Query: 67 KRFGTYAFRLPDDNGEGGFWVSYEDPDTAGNKASYVKTKNLGGVSIMDLSMDD 225
+ F L ++NG+G +S T+G Y+K L G + +D+
Sbjct: 504 RAFSDMLVALTNNNGDGRIIISRTSSSTSGQHGGYIKYVMLTGAKLFSEVVDE 556
>At5g65630.1 68418.m08256 DNA-binding bromodomain-containing protein
similar to 5.9 kb fsh membrane protein [Drosophila
melanogaster] GI:157455; contains Pfam profile PF00439:
Bromodomain
Length = 590
Score = 27.1 bits (57), Expect = 7.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 13 NPNQNGKRPHLRKVNDPSKRFGTYA 87
NPN N +R + K D S FG+YA
Sbjct: 44 NPNPNFERSNSSKQCDDSSEFGSYA 68
>At4g25390.2 68417.m03653 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 497
Score = 27.1 bits (57), Expect = 7.1
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 13 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDNGEGGFWVSYEDPDTAGNKASYVKTKNLG 192
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 193 GV 198
+
Sbjct: 133 SL 134
>At4g25390.1 68417.m03652 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 651
Score = 27.1 bits (57), Expect = 7.1
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 13 NPNQNGKRPHLRKVNDPSKRFGTYAFRLPDDNGEGGFWVSYEDPDTAGNKASYVKTKNLG 192
N + + +RP R+ + S R T +F + G+GGF V + + G + VK + G
Sbjct: 74 NASSSPRRPSPREFSYSSLRRATGSFSQANRLGQGGFGVVFRGTISGGENVA-VKVMDSG 132
Query: 193 GV 198
+
Sbjct: 133 SL 134
>At5g24120.1 68418.m02835 RNA polymerase sigma subunit SigE (sigE) /
sigma-like factor (SIG5) identical to RNA polymerase
sigma subunit SigE [Arabidopsis thaliana] GI:4972299,
sigma-like factor [Arabidopsis thaliana] GI:4033838;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2
Length = 517
Score = 26.6 bits (56), Expect = 9.4
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +1
Query: 193 GVSIMDLSMDDFRGLCTG-DKYPILRAAKYRL*TFSLYF*KHAL 321
G DL RGL T D++ R K+RL T+ L++ +HA+
Sbjct: 305 GPKFQDLCQAGMRGLITAIDRFEPKR--KFRLSTYGLFWIRHAI 346
>At4g27690.1 68417.m03981 vacuolar protein sorting-associated
protein 26, putative / VPS26, putative similar to
vacuolar sorting protein 26 [Homo sapiens] GI:9622852;
contains Pfam profile PF03643: Vacuolar protein
sorting-associated protein 26
Length = 303
Score = 26.6 bits (56), Expect = 9.4
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 255 IFISSAKTTKIIHGKVHDGHTAKVLCFHVTSFVSGRVRI 139
I S K K + K +G TA V FH +SG+V I
Sbjct: 16 ITFSDGKNRKQVPMKKENGQTALVPLFHSQDTISGKVCI 54
>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P49770 Translation initiation factor eIF-2B beta
subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF01008: Initiation factor 2
subunit family
Length = 407
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 178 TKNLGGVSIMDLSMDDFRGLCTGDKYPILRAA 273
T +GG+ ++D S DD C G +P + AA
Sbjct: 93 TAAMGGLDLLDASDDDDVDNCKGIGFPAMSAA 124
>At1g07650.1 68414.m00821 leucine-rich repeat transmembrane protein
kinase, putative similar to GB:AAC50043 from
[Arabidopsis thaliana] (Plant Mol. Biol. 37 (4), 587-596
(1998))
Length = 1014
Score = 26.6 bits (56), Expect = 9.4
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = -3
Query: 135 VRNPEATFTIIIRKAEGIGPKTFARIVDLTKMRTLSV 25
++N E+ T+I+RK + IGP I DL K++TL +
Sbjct: 277 LKNLESIKTLILRKCKIIGPIP-KYIGDLKKLKTLDL 312
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,100,227
Number of Sequences: 28952
Number of extensions: 210565
Number of successful extensions: 546
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 535
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 544
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -