Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_D03
(541 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 29 0.58
SPBC800.07c |tsf1||mitochondrial translation elongation factor E... 26 4.1
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 26 4.1
SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 26 4.1
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 26 4.1
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 5.4
SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccha... 25 9.5
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 25 9.5
SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi... 25 9.5
SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 25 9.5
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 28.7 bits (61), Expect = 0.58
Identities = 22/75 (29%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
Frame = -1
Query: 274 NGEDTTFLSFPDVYASLRSFIEVLSMASPKAVLLYSWSKK*NART-LQTAITANKYNLIM 98
N +DT+ L PD+ +S S E L P+ L+S + N+ T Q + T +K ++
Sbjct: 433 NNKDTSKLENPDISSSPLSPTEDLFPNDPEEENLFSAALGLNSNTGSQPSETQSKPSIDP 492
Query: 97 LTRPRVVDNQNTIMY 53
V DNQ+++++
Sbjct: 493 SESITVTDNQDSLLF 507
>SPBC800.07c |tsf1||mitochondrial translation elongation factor
EF-Ts Tsf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 299
Score = 25.8 bits (54), Expect = 4.1
Identities = 18/60 (30%), Positives = 31/60 (51%)
Frame = -1
Query: 436 IVKKSGFISEKVLLRLMYGLVASL*STSPKNLSISTRLPSPAPDL*RSISIITPNGEDTT 257
IVK + F EKV ++ ++ + A + ST+ S + SP L R S++ N + +T
Sbjct: 184 IVKMTSFTGEKVQVQRLHCMNARVPSTAIGIFSHGAKQSSPLQQLGRIGSMVQINSDLST 243
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 4.1
Identities = 14/52 (26%), Positives = 26/52 (50%)
Frame = +3
Query: 360 DYSEATNPYISLSKTFSEMNPDFFTMANKIYVGNKYTLDEKFTSSSRQYQSE 515
D +A + S SK F+E NP T ++ G +TL + T ++ ++ +
Sbjct: 141 DSLKALRQFFS-SKDFTETNPPIITSSDCEGAGEVFTLTPQETHKNKSFERD 191
>SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 510
Score = 25.8 bits (54), Expect = 4.1
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +3
Query: 288 LMLLYKSGAGEGSRVEIDKFLGDVDYSEATNP 383
L+ K G+G G + + FLGD+ Y + P
Sbjct: 243 LLAFDKIGSGSGDLSKCESFLGDILYMVSKEP 274
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.8 bits (54), Expect = 4.1
Identities = 18/53 (33%), Positives = 24/53 (45%)
Frame = -1
Query: 376 VASL*STSPKNLSISTRLPSPAPDL*RSISIITPNGEDTTFLSFPDVYASLRS 218
VAS TSP L+++ + PSP P S + +T T SLRS
Sbjct: 549 VASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSSPTKPSTTSLRS 601
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 5.4
Identities = 16/68 (23%), Positives = 31/68 (45%)
Frame = +3
Query: 246 KDKNVVSSPLGVMMLMLLYKSGAGEGSRVEIDKFLGDVDYSEATNPYISLSKTFSEMNPD 425
++ N + + + L ++ E + +D+ +V E+TN +L T + D
Sbjct: 367 EENNSLKQQIEQLQRELASETVVKENLKSSLDQQSANVQKLESTNR--ALESTIKTLEED 424
Query: 426 FFTMANKI 449
+TM NKI
Sbjct: 425 VYTMKNKI 432
>SPBC36.06c |spo9||farnesyl pyrophosphate
synthetase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 351
Score = 24.6 bits (51), Expect = 9.5
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = -1
Query: 307 DL*RSISIITPNGEDTTFLSFPDVYASLRSF 215
D R ++ +T G+ LS D ASLRSF
Sbjct: 154 DTFREVTFLTELGQQEDLLSSRDGEASLRSF 184
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/19 (52%), Positives = 13/19 (68%), Gaps = 1/19 (5%)
Frame = -2
Query: 183 LSCCTRGQRNRML-ARCRP 130
LSCCT G N +L ++C P
Sbjct: 1236 LSCCTEGSINALLNSKCLP 1254
>SPCC1183.04c |pet127||mitochondrial membrane protein
Pet127|Schizosaccharomyces pombe|chr 3|||Manual
Length = 524
Score = 24.6 bits (51), Expect = 9.5
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = +3
Query: 144 RAFYFFDHEYNRTALGDAIDKTSMKLLKEAYTSGKDKNVVSSPLGVMMLMLLYKS 308
+ FY +E N T L + + Y KDK++ P ++LM + KS
Sbjct: 158 KGFYISPNERNFTRLCRSASSVHISYQNGLYCIDKDKSLTKEPSVNIILMNVGKS 212
>SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 225
Score = 24.6 bits (51), Expect = 9.5
Identities = 14/49 (28%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = -1
Query: 256 FLSFPDVYASLR-SFIEVLSMASPKAVLLYSWSKK*NARTLQTAITANK 113
+ SFP + AS+ +F+ ++ + P V +Y+W+ LQ + +NK
Sbjct: 156 YSSFPLMIASIVCAFVPLIFIRIPVIVAMYAWTLFAAMGVLQNSNLSNK 204
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,180,896
Number of Sequences: 5004
Number of extensions: 43957
Number of successful extensions: 127
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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