Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_D01
(461 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann... 156 8e-39
At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann... 153 6e-38
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S... 85 2e-17
At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s... 82 2e-16
At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s... 81 3e-16
At1g67410.1 68414.m07672 exostosin family protein contains Pfam ... 32 0.16
At3g09560.2 68416.m01136 lipin family protein contains Pfam prof... 28 3.5
At3g09560.1 68416.m01135 lipin family protein contains Pfam prof... 28 3.5
At5g51020.1 68418.m06325 expressed protein similar to unknown pr... 27 4.7
At2g19050.1 68415.m02225 GDSL-motif lipase/hydrolase family prot... 27 4.7
At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family prot... 27 6.2
At1g66145.1 68414.m07507 CLE18, putative CLAVATA3/ESR-Related 18... 27 8.2
>At1g51590.1 68414.m05808 mannosyl-oligosaccharide
1,2-alpha-mannosidase, putative similar to
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
max][GI:6552504]
Length = 560
Score = 156 bits (378), Expect = 8e-39
Identities = 77/153 (50%), Positives = 109/153 (71%), Gaps = 1/153 (0%)
Frame = +2
Query: 5 HAWNNYKLYAWGKNELKPMSKRAHLSSVFGASDLGLTIVDGLSTLYLMGLNDEFREGRDW 184
HAW++Y+ YAWGK+EL+P +K + FG LG T+VD L TLY+MGL+++F++ R+W
Sbjct: 107 HAWSSYEKYAWGKDELQPRTKDG--TDSFGG--LGATMVDSLDTLYIMGLDEQFQKAREW 162
Query: 185 VAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGL 364
VA L F++ D + S+FETTIR +GGLLS Y L+GD +F +KA ++AD LLPA+ TPTG+
Sbjct: 163 VASSLDFDK-DYDASMFETTIRVVGGLLSAYDLSGDKMFLEKAKDIADRLLPAWNTPTGI 221
Query: 365 PYALINPSTKASKQYHW-AGPNSILSELGTLHL 460
PY +IN + W AG +SIL++ GT L
Sbjct: 222 PYNIINLRNGNAHNPSWAAGGDSILADSGTEQL 254
>At3g21160.1 68416.m02673 mannosyl-oligosaccharide
1,2-alpha-mannosidase, putative similar to
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
max][GI:6552504]
Length = 572
Score = 153 bits (371), Expect = 6e-38
Identities = 74/152 (48%), Positives = 108/152 (71%)
Frame = +2
Query: 5 HAWNNYKLYAWGKNELKPMSKRAHLSSVFGASDLGLTIVDGLSTLYLMGLNDEFREGRDW 184
HAW++Y+ YAWG++EL+P +K S FG LG T++D L TLY+MGL+++F++ R+W
Sbjct: 108 HAWSSYEKYAWGQDELQPQTKDGVDS--FGG--LGATMIDALDTLYIMGLDEQFQKAREW 163
Query: 185 VAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGL 364
VA L F++ D S+FETTIR +GGLLS Y L+GD +F +KA+++AD LLPA+ T +G+
Sbjct: 164 VASSLDFDK-DYAASMFETTIRVVGGLLSAYDLSGDKIFLEKAMDIADRLLPAWDTQSGI 222
Query: 365 PYALINPSTKASKQYHWAGPNSILSELGTLHL 460
PY +IN + WAG +SIL++ GT L
Sbjct: 223 PYNIINLKHGNAHNPTWAGGDSILADSGTEQL 254
>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
Similar to gb|U04299 mannosyl-oligosaccharide
alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
and gb|AA394707 come from this gene
Length = 574
Score = 85.0 bits (201), Expect = 2e-17
Identities = 59/178 (33%), Positives = 99/178 (55%), Gaps = 28/178 (15%)
Frame = +2
Query: 5 HAWNNYKLYAWGKNELKPMSKR-------------AHLSSVFGASDLGLTIVDGLSTLYL 145
HA++NY YA+ +ELKP++K HL + + S +T+V+ LS+L +
Sbjct: 47 HAYDNYMTYAFPHDELKPLTKSFTDSLSELGNLKLEHLPTDYNGS--AVTLVESLSSLAI 104
Query: 146 MGLNDEFREGRDWVAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDT-------VFR 304
+G + EF +G W++E+L F ++D +++FE IR +GGL+S + L D +
Sbjct: 105 LGNSTEFEKGVLWLSENLTF-DIDARVNLFECNIRVLGGLISAHLLAIDPNNRLIQGSYN 163
Query: 305 DKAVEVADAL----LPAFQTPTGLPYALINPST----KASKQYHWAGPNSILSELGTL 454
++ + +A+ L LPAF+TPTGLPYA IN + + +G S++ E+G L
Sbjct: 164 NQLLRLAEDLGKRFLPAFETPTGLPYAWINLKNGVMENETTETSTSGCGSLVLEMGAL 221
>At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein
similar to GI:5579331 from [Homo sapiens]; contains Pfam
profile PF01532: Glycosyl hydrolase family 47
Length = 624
Score = 82.2 bits (194), Expect = 2e-16
Identities = 52/138 (37%), Positives = 76/138 (55%), Gaps = 16/138 (11%)
Frame = +2
Query: 5 HAWNNYKLYAWGKNELKPMSKRAHLSSVFGASDLGLTIVDGLSTLYLMGLNDEFREGRDW 184
HAW+ Y+ YA G +EL P+S++ V G LG T+VD L T +MGL++ E W
Sbjct: 138 HAWSGYRKYAMGYDELMPISQKG----VDGLGGLGATVVDALDTAMIMGLDNIVSEAGSW 193
Query: 185 VAEH-LHFNEVDTELSVFETTIRFIGGLLSCYSLTG--------------DTVFRDKAVE 319
V H L ++++FETTIR +GGLLS Y L+G ++ + A +
Sbjct: 194 VETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSGGEQGTVNMTHVGPKPVIYLNIAKD 253
Query: 320 VADALLPAF-QTPTGLPY 370
+AD LL AF +PT +P+
Sbjct: 254 LADRLLSAFTSSPTPVPF 271
>At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein
similar to mannosyl-oligosaccharide
1,2-alpha-mannosidase IB [Mus musculus][SP|P39098]
Length = 624
Score = 81.4 bits (192), Expect = 3e-16
Identities = 55/165 (33%), Positives = 87/165 (52%), Gaps = 15/165 (9%)
Frame = +2
Query: 5 HAWNNYKLYAWGKNELKPMSKRAHLSSVFGASDLGLTIVDGLSTLYLMGLNDEFREGRDW 184
HA++ Y A+ +EL+P+S + G LT++D L TL L+G + F +W
Sbjct: 50 HAFDGYMNNAFPLDELRPLSCQGE--DTLGG--YALTLIDSLDTLALLGDRERFTSSVEW 105
Query: 185 VAEHLHFNEVDTELSVFETTIRFIGGLLSCYSLTGDTV-------FRDKAVEVADAL--- 334
+ ++L FN ++ +SVFETTIR +GGLLS + + D + ++ + +A+ L
Sbjct: 106 IGKNLQFN-INKTVSVFETTIRVLGGLLSAHLIASDYATGMRIPSYNNELLVLAENLARR 164
Query: 335 -LPAFQTPTGLPYALIN----PSTKASKQYHWAGPNSILSELGTL 454
LPAF TPTG+P+ +N SK AG ++ E G L
Sbjct: 165 MLPAFDTPTGIPFGSVNLMYGVDKHESKITSTAGGGTLSLEFGVL 209
>At1g67410.1 68414.m07672 exostosin family protein contains Pfam
profile: PF03016 Exostosin family
Length = 430
Score = 32.3 bits (70), Expect = 0.16
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = +3
Query: 168 ERDETGSPNICISTKSIRSYRYSRQPSGSSVDSCRVIR*PVTQC 299
E+ + NI +ST+ +RS ++ P+G + SCR+ V+ C
Sbjct: 281 EKSVATTQNIKVSTEGMRSSKFCLHPAGDTPSSCRLFDAIVSHC 324
>At3g09560.2 68416.m01136 lipin family protein contains Pfam
profile: PF04571 lipin, N-terminal conserved region
Length = 904
Score = 27.9 bits (59), Expect = 3.5
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = -2
Query: 256 DEPDGCLEYR*LRIDFVEMQMFGDPVSSLSELI---IESHQVESAESVYDGESE 104
D P EY R D + ++ +GD S SE++ I+ H + + SV + E+E
Sbjct: 159 DPPSPTSEYGSARFDNLNVESYGDSQGSDSEVVLVSIDGHILTAPVSVAEQEAE 212
>At3g09560.1 68416.m01135 lipin family protein contains Pfam
profile: PF04571 lipin, N-terminal conserved region
Length = 904
Score = 27.9 bits (59), Expect = 3.5
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = -2
Query: 256 DEPDGCLEYR*LRIDFVEMQMFGDPVSSLSELI---IESHQVESAESVYDGESE 104
D P EY R D + ++ +GD S SE++ I+ H + + SV + E+E
Sbjct: 159 DPPSPTSEYGSARFDNLNVESYGDSQGSDSEVVLVSIDGHILTAPVSVAEQEAE 212
>At5g51020.1 68418.m06325 expressed protein similar to unknown
protein (pir||S76207)
Length = 269
Score = 27.5 bits (58), Expect = 4.7
Identities = 16/66 (24%), Positives = 30/66 (45%)
Frame = -2
Query: 316 HCFIPEHCVTGQRITRQESTDEPDGCLEYR*LRIDFVEMQMFGDPVSSLSELIIESHQVE 137
H + +TG++ TR++++ +PD R L EM + G V L + + Q
Sbjct: 48 HARVVSRSLTGEKFTREQASRDPDNYFNIRMLSCPAAEM-VDGSEVLYLEQAFWRTPQKP 106
Query: 136 SAESVY 119
+ +Y
Sbjct: 107 FRQRLY 112
>At2g19050.1 68415.m02225 GDSL-motif lipase/hydrolase family protein
low similarity to SP|P40603 Anter-specific proline-rich
protein APG (Protein CEX) (Fragment) {Brassica napus};
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 349
Score = 27.5 bits (58), Expect = 4.7
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +2
Query: 227 SVFETTIRFIGGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGLPYA 373
+ F TT +G + CY + GD+VF + V + +P G+ +A
Sbjct: 16 TAFATTEAALGQRVPCYFVFGDSVFDNGNNNVLNTSAKVNYSPYGIDFA 64
>At2g19060.1 68415.m02226 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL6 GI:15054390, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 349
Score = 27.1 bits (57), Expect = 6.2
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 257 GGLLSCYSLTGDTVFRDKAVEVADALLPAFQTPTGLPYA 373
G L+ CY + GD+VF + D L +P G+ +A
Sbjct: 25 GQLVPCYFVFGDSVFDNGNNNELDTLAKVNYSPYGIDFA 63
>At1g66145.1 68414.m07507 CLE18, putative CLAVATA3/ESR-Related 18
(CLE18)
Length = 101
Score = 26.6 bits (56), Expect = 8.2
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +2
Query: 347 QTPTGLPYALINPSTKASKQYHWAG 421
Q PTG P L NP + K +HW G
Sbjct: 38 QIPTG-PDPLHNPPQPSPKHHHWIG 61
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,755,160
Number of Sequences: 28952
Number of extensions: 185093
Number of successful extensions: 546
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 535
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 772134480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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