Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_B22
(589 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 31 0.12
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 27 2.0
SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein L10a... 26 4.7
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 26 4.7
SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe... 26 4.7
SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|ch... 26 4.7
SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 26 4.7
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 6.2
SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4 |Schiz... 25 6.2
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 25 8.2
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 25 8.2
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 31.1 bits (67), Expect = 0.12
Identities = 23/89 (25%), Positives = 39/89 (43%)
Frame = -3
Query: 290 RSQEDLDNGISGNIGLNVDGNTERLVVESWLTNLEVVWVTSLNLFFGQEYTVSGIKLAIV 111
R QED + G N+ G E + E ++ L F G+ + +S + I+
Sbjct: 1109 RCQEDFERGWKANLPSGKAGEAEIMSDEYYVAAAIKRRGLGLVRFIGELFKLSMLSEKIM 1168
Query: 110 KECD*FLNDNIIDFNADEMKFLLRIRLQV 24
EC L N+ D +E++ L R+ + V
Sbjct: 1169 HECIKRLLGNVTDPEEEEIESLCRLLMTV 1197
>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 796
Score = 27.1 bits (57), Expect = 2.0
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +3
Query: 270 IKIFLGPKYNDXGFPITLEENWHKFYELDWFTHKIT 377
+ I L P ND + T WHKF++ WF +T
Sbjct: 416 LHIELAPAANDIQWHNTYIGRWHKFFQ-GWFITLVT 450
>SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein
L10a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 4.7
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -1
Query: 484 GTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGV 374
G FPS + + GK + +KS F+L+ +L C GV
Sbjct: 131 GKFPSPVSHADDLYGKITEVKSTIKFQLKKVL-CLGV 166
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +3
Query: 342 FYELDWFTHKITPGQNKIVRNSNE--FSLFKEDSLPXTDLMKLLD 470
F LDWFT+ + QN+++R S + F + L +KLL+
Sbjct: 654 FPTLDWFTNDSSVIQNELLRRSVDTYFRYIFQTDLKLEQRIKLLE 698
>SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 426
Score = 25.8 bits (54), Expect = 4.7
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 289 LSTTXMDSPSHWKKTGINSTNWIG 360
LS + S+W++T ++ TNW+G
Sbjct: 208 LSVKAIIFASYWQQTVLSITNWLG 231
>SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 202
Score = 25.8 bits (54), Expect = 4.7
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -1
Query: 523 LFGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFE 404
LFG +KP+ GT +SNN +G + +EN E
Sbjct: 160 LFGGGNKPAAAVTGTSSNSNNASAKSDGPTITELNENETE 199
>SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.8 bits (54), Expect = 4.7
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +3
Query: 291 KYNDXGFPITLEENWHKFYELDWFTHKITPGQNKIVRNSNEFSLFKEDSLPXTD 452
KYN+ P H ++L+W T N + R+ EFS+F D LP T+
Sbjct: 147 KYNNTMIP-----GAHCTFKLNWATGGGIQHNNFVSRDP-EFSIFVGDLLPTTE 194
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 25.4 bits (53), Expect = 6.2
Identities = 14/51 (27%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = -3
Query: 245 LNVDGNTERLVVESWLTNLEVVWVTSLNLFFGQEYTVSGI---KLAIVKEC 102
LN+ TE ++ ++ +V V S N FFG + G+ K ++ ++C
Sbjct: 309 LNITRKTETILKSLKESSTPLVQVVSFNAFFGVMIAILGVQFFKASLNRQC 359
>SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 674
Score = 25.4 bits (53), Expect = 6.2
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +3
Query: 90 EKLVTFFDYSQFDATNSVFLTKKEIKTSYPHNFKVRQPRLNHKPFSVTID-VKSDIATD 263
E++V + QF+A + T+K + + P K R+ RL F +D + D ATD
Sbjct: 591 ERVVLLEAWKQFEAMHGTEDTRKHVSSLMPQVVKKRR-RLEDGSFEEYLDYLFPDTATD 648
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 25.0 bits (52), Expect = 8.2
Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = -1
Query: 337 CQFSSNVMGNPXSLYLGPKKILITASVAISDLTSMV-TLKGLWLSRG 200
CQF V+G L+ K LI+ S A L+++ + KG LS G
Sbjct: 113 CQFWETVLGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMG 159
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 25.0 bits (52), Expect = 8.2
Identities = 22/83 (26%), Positives = 34/83 (40%)
Frame = -1
Query: 475 PSSNNFMRSVNGKESSLKSENSFELRTILFCPGVILCVNQSSS*NLCQFSSNVMGNPXSL 296
PS + F + + S S N F + IL P L SS F +N P S+
Sbjct: 249 PSYSIFPGQIVSVKGSNPSGNMFIAKEILPIPP--LPFPSSSKQEHATFVANTNNQPISI 306
Query: 295 YLGPKKILITASVAISDLTSMVT 227
Y+ + ++ S L SM++
Sbjct: 307 YIASGPWSLRDDLSFSPLKSMIS 329
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,417,334
Number of Sequences: 5004
Number of extensions: 49408
Number of successful extensions: 157
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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