Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_A23
(429 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9SMV2 Cluster: AT-LS1 product; n=18; Magnoliophyta|Rep... 52 5e-06
UniRef50_Q1ZZP4 Cluster: Macrophage migration inhibitory factor-... 52 5e-06
UniRef50_UPI0000E473B2 Cluster: PREDICTED: similar to macrophage... 48 6e-05
UniRef50_A2I461 Cluster: Macrophage migration inhibitory factor-... 48 6e-05
UniRef50_Q6FHV0 Cluster: MIF protein; n=6; Eutheria|Rep: MIF pro... 48 8e-05
UniRef50_P14174 Cluster: Macrophage migration inhibitory factor;... 46 3e-04
UniRef50_Q9U228 Cluster: Putative uncharacterized protein mif-1;... 46 4e-04
UniRef50_A7SF14 Cluster: Predicted protein; n=1; Nematostella ve... 45 8e-04
UniRef50_P30904 Cluster: Macrophage migration inhibitory factor;... 45 8e-04
UniRef50_P34884 Cluster: Macrophage migration inhibitory factor;... 44 0.001
UniRef50_Q6IQL4 Cluster: Zgc:86714; n=10; Euteleostomi|Rep: Zgc:... 43 0.003
UniRef50_Q18785 Cluster: MIF-like protein mif-2; n=3; Rhabditida... 42 0.004
UniRef50_A1XDS9 Cluster: MIF; n=1; Toxoplasma gondii|Rep: MIF - ... 39 0.051
UniRef50_A4S5V7 Cluster: Predicted protein; n=2; Ostreococcus|Re... 38 0.067
UniRef50_A6R7K5 Cluster: Predicted protein; n=1; Ajellomyces cap... 38 0.067
UniRef50_P30046 Cluster: D-dopachrome decarboxylase; n=15; Tetra... 37 0.15
UniRef50_A5ATL9 Cluster: Putative uncharacterized protein; n=1; ... 36 0.36
UniRef50_Q2JNV6 Cluster: Conserved domain protein; n=8; Cyanobac... 36 0.47
UniRef50_Q319W9 Cluster: Macrophage migration inhibitory factor ... 35 0.82
UniRef50_Q8YZX4 Cluster: Alr0331 protein; n=4; Cyanobacteria|Rep... 34 1.4
UniRef50_Q7RJK4 Cluster: DNA-directed RNA polymerase; n=13; Euka... 33 3.3
UniRef50_Q9X180 Cluster: Sensor protein; n=2; Thermotoga|Rep: Se... 32 5.8
UniRef50_Q7U982 Cluster: Possible ATLS1-like light-inducible pro... 32 5.8
UniRef50_UPI0000D557C9 Cluster: PREDICTED: similar to CG14880-PA... 31 7.7
UniRef50_Q650N8 Cluster: Putative uncharacterized protein; n=3; ... 31 7.7
>UniRef50_Q9SMV2 Cluster: AT-LS1 product; n=18; Magnoliophyta|Rep:
AT-LS1 product - Arabidopsis thaliana (Mouse-ear cress)
Length = 115
Score = 52.0 bits (119), Expect = 5e-06
Identities = 27/69 (39%), Positives = 38/69 (55%)
Frame = +1
Query: 139 PISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNY 318
P+SFGG + P A L SIG L NK+++ VSA ++ +L + K RFFL FY+
Sbjct: 48 PMSFGG-TEDPAAYGELVSIGGLNADVNKKLSAAVSAILDTKLSVPKSRFFLKFYETKGS 106
Query: 319 NVAKGGITI 345
G T+
Sbjct: 107 FFGWNGATL 115
>UniRef50_Q1ZZP4 Cluster: Macrophage migration inhibitory
factor-like protein; n=1; Acyrthosiphon pisum|Rep:
Macrophage migration inhibitory factor-like protein -
Acyrthosiphon pisum (Pea aphid)
Length = 119
Score = 52.0 bits (119), Expect = 5e-06
Identities = 23/60 (38%), Positives = 37/60 (61%)
Frame = +1
Query: 175 AVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNYNVAKGGITIDLI 354
A+ L GN G+ ENK A+ + F+EK+LGI +D+F+L+F + N+ G T++ I
Sbjct: 58 ALGNLTGTGNFGIDENKHYASIIYDFIEKQLGIPQDKFYLSFVEQKPSNIGVRGTTLEEI 117
>UniRef50_UPI0000E473B2 Cluster: PREDICTED: similar to macrophage
migration inhibitory factor; n=2; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to macrophage
migration inhibitory factor - Strongylocentrotus
purpuratus
Length = 93
Score = 48.4 bits (110), Expect = 6e-05
Identities = 26/52 (50%), Positives = 36/52 (69%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDR 285
P+ +SF G ST P AVA + SIGNLG++ENK I ++A + K +G+K DR
Sbjct: 44 PNQMMSFAG-STEPCAVANVRSIGNLGLEENKVITQIITAEMTK-IGVKADR 93
>UniRef50_A2I461 Cluster: Macrophage migration inhibitory
factor-like protein; n=7; Coelomata|Rep: Macrophage
migration inhibitory factor-like protein -
Maconellicoccus hirsutus (hibiscus mealybug)
Length = 121
Score = 48.4 bits (110), Expect = 6e-05
Identities = 28/70 (40%), Positives = 37/70 (52%)
Frame = +1
Query: 133 DSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVL 312
D I +GG + P ATL SIG LG++ENK+ A + + K LGI DR ++ F D
Sbjct: 45 DQSIIWGG-TEEPCGYATLMSIGKLGIEENKKHAAAIYEHLLKHLGIPGDRMYINFVDSA 103
Query: 313 NYNVAKGGIT 342
V G T
Sbjct: 104 PSTVGYNGST 113
>UniRef50_Q6FHV0 Cluster: MIF protein; n=6; Eutheria|Rep: MIF
protein - Homo sapiens (Human)
Length = 115
Score = 48.0 bits (109), Expect = 8e-05
Identities = 22/65 (33%), Positives = 39/65 (60%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
PD ++FGG S+ P A+ +L SIG +G +N+ + ++ + + L I DR ++ +YD+
Sbjct: 44 PDQLMAFGG-SSEPCALCSLHSIGRIGGAQNRSYSKQLCGLLAERLRISPDRVYINYYDM 102
Query: 310 LNYNV 324
NV
Sbjct: 103 NAANV 107
>UniRef50_P14174 Cluster: Macrophage migration inhibitory factor;
n=12; Euteleostomi|Rep: Macrophage migration inhibitory
factor - Homo sapiens (Human)
Length = 115
Score = 46.0 bits (104), Expect = 3e-04
Identities = 22/65 (33%), Positives = 38/65 (58%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
PD ++FGG S+ P A+ +L SIG +G +N+ + + + + L I DR ++ +YD+
Sbjct: 44 PDQLMAFGG-SSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDM 102
Query: 310 LNYNV 324
NV
Sbjct: 103 NAANV 107
>UniRef50_Q9U228 Cluster: Putative uncharacterized protein mif-1;
n=2; Caenorhabditis|Rep: Putative uncharacterized
protein mif-1 - Caenorhabditis elegans
Length = 117
Score = 45.6 bits (103), Expect = 4e-04
Identities = 26/73 (35%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
Frame = +1
Query: 127 HPDSPISFGGESTSPGAVATLESIGNLG-VKENKEIANEVSAFVEKELGIKKDRFFLTFY 303
H D I + G +T P A L+SIG +G K+N I+ V +EK LGI +R ++ F
Sbjct: 44 HEDQGILYAG-TTEPAGFAVLKSIGGVGSAKQNNAISAVVFPIIEKHLGIPGNRLYIEFV 102
Query: 304 DVLNYNVAKGGIT 342
++ ++A G T
Sbjct: 103 NLGAADIAYNGQT 115
>UniRef50_A7SF14 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 115
Score = 44.8 bits (101), Expect = 8e-04
Identities = 22/71 (30%), Positives = 35/71 (49%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
P + FGG +T P A+ L +IG K + +S ++K LG+ DR ++ F+D
Sbjct: 44 PGLRLMFGG-TTEPAAIVNLTNIGQHDPATTKHRSKVISNHIQKTLGVPADRMYIIFHDK 102
Query: 310 LNYNVAKGGIT 342
+ V G T
Sbjct: 103 QRFEVGYNGAT 113
>UniRef50_P30904 Cluster: Macrophage migration inhibitory factor;
n=6; Rattus norvegicus|Rep: Macrophage migration
inhibitory factor - Rattus norvegicus (Rat)
Length = 115
Score = 44.8 bits (101), Expect = 8e-04
Identities = 23/71 (32%), Positives = 37/71 (52%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
PD ++F G S P A+ +L SIG +G +N+ + + + L I DR ++ +YD+
Sbjct: 44 PDQLMTFSGTS-DPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDM 102
Query: 310 LNYNVAKGGIT 342
NV G T
Sbjct: 103 NAANVGWNGST 113
>UniRef50_P34884 Cluster: Macrophage migration inhibitory factor;
n=21; Vertebrata|Rep: Macrophage migration inhibitory
factor - Mus musculus (Mouse)
Length = 115
Score = 44.0 bits (99), Expect = 0.001
Identities = 22/71 (30%), Positives = 37/71 (52%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
PD ++F G + P A+ +L SIG +G +N+ + + + L I DR ++ +YD+
Sbjct: 44 PDQLMTFSG-TNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDM 102
Query: 310 LNYNVAKGGIT 342
NV G T
Sbjct: 103 NAANVGWNGST 113
>UniRef50_Q6IQL4 Cluster: Zgc:86714; n=10; Euteleostomi|Rep:
Zgc:86714 - Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 118
Score = 42.7 bits (96), Expect = 0.003
Identities = 23/76 (30%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
Frame = +1
Query: 130 PDSPISFGGESTSPGAVATLESIGNLGVKE-NKEIANEVSAFVEKELGIKKDRFFLTFYD 306
PD P+ F G S+SP + T+ +IG E NK+ + ++ F++ E G+ DR + FY
Sbjct: 44 PDLPMFFAG-SSSPCVLMTVSAIGVTDTAEKNKQHSAKIFQFLQGEFGLSDDRILVLFYP 102
Query: 307 VLNYNVAKGGITIDLI 354
+ + K G + +
Sbjct: 103 LEPSQIGKKGTVMSFL 118
>UniRef50_Q18785 Cluster: MIF-like protein mif-2; n=3;
Rhabditida|Rep: MIF-like protein mif-2 - Caenorhabditis
elegans
Length = 120
Score = 42.3 bits (95), Expect = 0.004
Identities = 20/64 (31%), Positives = 35/64 (54%)
Frame = +1
Query: 154 GESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNYNVAKG 333
G + P V +++SIG + ++N ++ F KELG+ KD+ +TF+D+ V
Sbjct: 51 GATHDPVTVISIKSIGAVSAEDNIRNTAAITEFCGKELGLPKDKVVITFHDLPPATVGFN 110
Query: 334 GITI 345
G T+
Sbjct: 111 GTTV 114
>UniRef50_A1XDS9 Cluster: MIF; n=1; Toxoplasma gondii|Rep: MIF -
Toxoplasma gondii
Length = 116
Score = 38.7 bits (86), Expect = 0.051
Identities = 21/51 (41%), Positives = 30/51 (58%)
Frame = +1
Query: 148 FGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTF 300
FGG S+ P A + SIG + N +IA +SA E+ LG+ K+R + TF
Sbjct: 51 FGG-SSDPCAFIRVASIGGITSSTNCKIAAALSAACERHLGVPKNRIYTTF 100
>UniRef50_A4S5V7 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 146
Score = 38.3 bits (85), Expect = 0.067
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +1
Query: 154 GESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNYNVAKG 333
G S A+ L S+G + ++ NK ++ +V A + + GI R ++TF DV N+
Sbjct: 81 GGSDDDCALCRLTSLGGIDLENNKAVSEDVCALLGETFGIAGTRVYVTFEDVARENMGYD 140
Query: 334 GIT 342
T
Sbjct: 141 SAT 143
>UniRef50_A6R7K5 Cluster: Predicted protein; n=1; Ajellomyces
capsulatus NAm1|Rep: Predicted protein - Ajellomyces
capsulatus NAm1
Length = 200
Score = 38.3 bits (85), Expect = 0.067
Identities = 20/78 (25%), Positives = 40/78 (51%), Gaps = 1/78 (1%)
Frame = +1
Query: 64 KLYRFYRRQSRRCLKNLFVS*HPDSPISFGGESTSPGAVATLESIGNLGVKENKEIANEV 243
K RF+R +R + L + + D P++F ++T+P + + G +G E ++ +
Sbjct: 62 KFLRFHRPFNREVSQTLTIENNNDEPVAFKVKTTAPKSYCVRPNSGRIGAGERIDVQVLL 121
Query: 244 SAFV-EKELGIKKDRFFL 294
A +K +G KD+F +
Sbjct: 122 QAMKDDKSVGASKDKFLV 139
>UniRef50_P30046 Cluster: D-dopachrome decarboxylase; n=15;
Tetrapoda|Rep: D-dopachrome decarboxylase - Homo sapiens
(Human)
Length = 118
Score = 37.1 bits (82), Expect = 0.15
Identities = 22/80 (27%), Positives = 41/80 (51%), Gaps = 1/80 (1%)
Frame = +1
Query: 118 VS*HPDSPISFGGESTSPGAVATLESIGNLGVKE-NKEIANEVSAFVEKELGIKKDRFFL 294
V+ P ++ G ST P A ++ SIG +G E N+ + F+ KEL + +DR +
Sbjct: 40 VTVRPGLAMALSG-STEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILI 98
Query: 295 TFYDVLNYNVAKGGITIDLI 354
F+ + ++ + K G + +
Sbjct: 99 RFFPLESWQIGKIGTVMTFL 118
>UniRef50_A5ATL9 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 219
Score = 35.9 bits (79), Expect = 0.36
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 142 ISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYD-VLNY 318
ISF G P A A + S+G + + +++ + ++ +L I + RFFL YD + +
Sbjct: 156 ISFEGNK-EPAAFAEIISMGGINSEVKRKLIATIGTILQTKLSIPRTRFFLKVYDTTMAH 214
Query: 319 NVAK 330
+AK
Sbjct: 215 KIAK 218
>UniRef50_Q2JNV6 Cluster: Conserved domain protein; n=8;
Cyanobacteria|Rep: Conserved domain protein -
Synechococcus sp. (strain JA-2-3B'a(2-13))
(Cyanobacteria bacteriumYellowstone B-Prime)
Length = 116
Score = 35.5 bits (78), Expect = 0.47
Identities = 19/68 (27%), Positives = 31/68 (45%)
Frame = +1
Query: 139 PISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNY 318
P++F G S P ++SIG ++ + ++ +E LGI K R ++ F D Y
Sbjct: 48 PMTFAG-SGDPCCYLEIKSIGQFSAQQTRAMSEFFCGTIEARLGIPKKRIYIEFSDAKGY 106
Query: 319 NVAKGGIT 342
G T
Sbjct: 107 LWGWNGTT 114
>UniRef50_Q319W9 Cluster: Macrophage migration inhibitory factor
family; n=6; Prochlorococcus marinus|Rep: Macrophage
migration inhibitory factor family - Prochlorococcus
marinus (strain MIT 9312)
Length = 110
Score = 34.7 bits (76), Expect = 0.82
Identities = 19/51 (37%), Positives = 30/51 (58%)
Frame = +1
Query: 157 ESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDV 309
E SP ++SIG+L EIA +S FV +++GI D+ +++F DV
Sbjct: 50 EDESPCCFLEIKSIGSL---TPSEIAKPISNFVYEKIGIPIDKIYISFEDV 97
>UniRef50_Q8YZX4 Cluster: Alr0331 protein; n=4; Cyanobacteria|Rep:
Alr0331 protein - Anabaena sp. (strain PCC 7120)
Length = 761
Score = 33.9 bits (74), Expect = 1.4
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = +1
Query: 190 ESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNYNVAKGGITIDLIEP 360
E + N + E E+AN + V EL I D F+ ++V YN K G + L+EP
Sbjct: 129 ELVLNGALFEEIEVANYSTTTVAFELSISFDADFVDLFEVRGYNRDKRGKLLRLVEP 185
>UniRef50_Q7RJK4 Cluster: DNA-directed RNA polymerase; n=13;
Eukaryota|Rep: DNA-directed RNA polymerase - Plasmodium
yoelii yoelii
Length = 2594
Score = 32.7 bits (71), Expect = 3.3
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +1
Query: 223 KEIANEVSAFVEKELGIKKDRFFLTF 300
K I N+ + F +KEL +KKD +F+TF
Sbjct: 1159 KNIPNKFNLFNDKELNLKKDLYFITF 1184
>UniRef50_Q9X180 Cluster: Sensor protein; n=2; Thermotoga|Rep:
Sensor protein - Thermotoga maritima
Length = 755
Score = 31.9 bits (69), Expect = 5.8
Identities = 20/50 (40%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
Frame = -1
Query: 360 WFN*IDGDAAFRDVVVQDIVE-SKEKSVLFNTEFLFNEC*HFIRNLFVLF 214
+ N I + + + +V++I+E SKE+ VL TEF NE IR ++VLF
Sbjct: 576 YINIITNELSRLETIVKEILEYSKERQVLEFTEFNLNE---LIREVYVLF 622
>UniRef50_Q7U982 Cluster: Possible ATLS1-like light-inducible
protein; n=6; Cyanobacteria|Rep: Possible ATLS1-like
light-inducible protein - Synechococcus sp. (strain
WH8102)
Length = 131
Score = 31.9 bits (69), Expect = 5.8
Identities = 22/68 (32%), Positives = 34/68 (50%)
Frame = +1
Query: 139 PISFGGESTSPGAVATLESIGNLGVKENKEIANEVSAFVEKELGIKKDRFFLTFYDVLNY 318
P++F G S P A ++SIG L ++++ ++ LGI KDR ++ F DV
Sbjct: 66 PMTFAG-SEEPCAYVEVKSIGAL---TPPAMSDQFCELIKSSLGIPKDRIYIGFDDVNAS 121
Query: 319 NVAKGGIT 342
N G T
Sbjct: 122 NWGWNGRT 129
>UniRef50_UPI0000D557C9 Cluster: PREDICTED: similar to CG14880-PA,
isoform A; n=1; Tribolium castaneum|Rep: PREDICTED:
similar to CG14880-PA, isoform A - Tribolium castaneum
Length = 428
Score = 31.5 bits (68), Expect = 7.7
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = -2
Query: 275 LIPSSFSTNADTSFAISLFSLTPKFPIDSRVATAPGLVDSPP 150
L+P S ST + +F + TP FP+ V AP L PP
Sbjct: 223 LVPPSGSTESSVNFESRFKATTPVFPLSVDVTEAPNLGLLPP 264
>UniRef50_Q650N8 Cluster: Putative uncharacterized protein; n=3;
Bacteroides fragilis|Rep: Putative uncharacterized
protein - Bacteroides fragilis
Length = 355
Score = 31.5 bits (68), Expect = 7.7
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = +2
Query: 68 YTGFIEDSQEGA*KIYLCRNTLTALYRSAGNRRVPAPLLLWNQSEI*VLK 217
Y F E +Q G IY+ N++ AL S GN +P+ + +++ + I + K
Sbjct: 268 YVVFNETTQIGFEDIYVTNNSIYALLHSIGNETLPSEITVFDWAGIPITK 317
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 375,377,116
Number of Sequences: 1657284
Number of extensions: 6626352
Number of successful extensions: 19191
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 18836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19188
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 20653970351
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -