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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0001_A14
         (545 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_18399| Best HMM Match : AMP-binding (HMM E-Value=0)                 74   7e-14
SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0)                  48   7e-06
SB_18329| Best HMM Match : AMP-binding (HMM E-Value=2.7e-18)           40   0.001
SB_54379| Best HMM Match : AMP-binding (HMM E-Value=0.0023)            36   0.029
SB_53246| Best HMM Match : AMP-binding (HMM E-Value=7.8e-37)           35   0.050
SB_16214| Best HMM Match : AMP-binding (HMM E-Value=0)                 35   0.050
SB_57182| Best HMM Match : AMP-binding (HMM E-Value=0)                 34   0.066
SB_39565| Best HMM Match : No HMM Matches (HMM E-Value=.)              34   0.066
SB_36835| Best HMM Match : AMP-binding (HMM E-Value=6.2e-13)           33   0.20 
SB_7062| Best HMM Match : AMP-binding (HMM E-Value=1.4e-18)            31   0.46 
SB_19092| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.81 
SB_57048| Best HMM Match : S-antigen (HMM E-Value=3.4)                 29   2.5  
SB_19482| Best HMM Match : Pkinase_Tyr (HMM E-Value=2.4e-11)           29   2.5  
SB_57704| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.3  
SB_10020| Best HMM Match : Extensin_2 (HMM E-Value=0.88)               29   3.3  
SB_45441| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   4.3  
SB_43771| Best HMM Match : AMP-binding (HMM E-Value=0.041)             28   4.3  
SB_13709| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   4.3  
SB_10665| Best HMM Match : PT (HMM E-Value=6.2)                        28   5.7  
SB_31957| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.7  
SB_42135| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   7.6  

>SB_18399| Best HMM Match : AMP-binding (HMM E-Value=0)
          Length = 1381

 Score = 74.1 bits (174), Expect = 7e-14
 Identities = 55/179 (30%), Positives = 87/179 (48%), Gaps = 5/179 (2%)
 Frame = +3

Query: 24  VPIGVPISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGY--VRGKDPHRFIHNPHNTH 197
           + IG    N   ++LD  +  V   + GE+C++G  LARGY  +  K   +F+   H  H
Sbjct: 341 ITIGRAFGNYNAHILDSILVDVEGEKEGELCISGKCLARGYRNLPEKTKQQFVD--HQLH 398

Query: 198 PNFAKLYRTGDF-GILDKGVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLC 374
               ++YRTGD       G I Y GR D QVKIRG RV++  ++  +     V    V+ 
Sbjct: 399 ---GRIYRTGDLVRWTPDGEIEYLGRIDDQVKIRGFRVEIGSIETHLQRQENVKMAAVVV 455

Query: 375 YGVESSNPEILAFVTINPEAR-MSAKNIEAELTSSLTDYMIPQ-VIEIETVPVLVNGKV 545
                    ++A +T+  + +   A+    ++  +L +YMIP   I  E +P L +GKV
Sbjct: 456 LSPSPGQQLVVAHLTLVDKTKGFQAQQAVVKMKIALPEYMIPNGYIIHEELPTLASGKV 514


>SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0)
          Length = 445

 Score = 47.6 bits (108), Expect = 7e-06
 Identities = 37/146 (25%), Positives = 62/146 (42%), Gaps = 3/146 (2%)
 Frame = +3

Query: 57  VYLLDENMRCVREGEPGEVCVAGFNLARGYVRG--KDPHRFIHNPHNTHPNFAKLYRTGD 230
           V +LD   + V  GE G + +    L  G +    ++  RF     NT+  +   Y +GD
Sbjct: 301 VQILDAKGKPVAAGEQGSIAIK-LPLPPGCLPTIWRNHERFEAGYLNTYKGY---YVSGD 356

Query: 231 FGILDK-GVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEIL 407
            G+LD  G +   GR D  + + GHR+   E++  V+    V  C V+         E L
Sbjct: 357 GGMLDNDGYLFVLGRTDDIINVAGHRLSTGEMEEVVAGHDAVAECAVIGQNSALKGEEPL 416

Query: 408 AFVTINPEARMSAKNIEAELTSSLTD 485
             V +     +  + ++ EL   + D
Sbjct: 417 GLVLLKDGVDIDEEQLKKELVQRVRD 442


>SB_18329| Best HMM Match : AMP-binding (HMM E-Value=2.7e-18)
          Length = 1076

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 25/107 (23%), Positives = 52/107 (48%)
 Frame = +3

Query: 24  VPIGVPISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPN 203
           +PIG P+ +  V + DE  + +  G           + + Y+ G +   F++N  +  P 
Sbjct: 294 IPIGEPLQDTLVDIRDEEGKKISHG-----------IGQMYIGGHNRICFLNNEMDVSPG 342

Query: 204 FAKLYRTGDFGILDKGVILYAGRADSQVKIRGHRVDLQEVDHAVSTI 344
             +   +GD+ +++   I + GR D Q+K  G R +L  ++ A++ +
Sbjct: 343 TMRA--SGDWALINNDQIWFLGRKDRQIKRLGQRTNLLWIEQALARV 387


>SB_54379| Best HMM Match : AMP-binding (HMM E-Value=0.0023)
          Length = 277

 Score = 35.5 bits (78), Expect = 0.029
 Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 1/110 (0%)
 Frame = +3

Query: 96  GEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFAKLYRTGDFGILDKGVILY-AGR 272
           G+ GE+C+ G  + +GY+   +         NT  +   L+ TGD G  D     Y  GR
Sbjct: 112 GKQGEICIKGPLMMKGYLNNPEA------TANTIDHEGWLH-TGDIGYYDDQEHFYIVGR 164

Query: 273 ADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEILAFVTI 422
               +K +G +V   E++  + + P + +  V+    E +     AFV +
Sbjct: 165 VKELIKYKGFQVPPAELEDLLQSHPDIADAAVIGVPDEEAGELPKAFVVL 214


>SB_53246| Best HMM Match : AMP-binding (HMM E-Value=7.8e-37)
          Length = 497

 Score = 34.7 bits (76), Expect = 0.050
 Identities = 35/130 (26%), Positives = 55/130 (42%), Gaps = 3/130 (2%)
 Frame = +3

Query: 42  ISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTH--PNFAKL 215
           +  N V ++ +  + VR G PGE+CV    L + YV          NP  T         
Sbjct: 308 LPGNEVKIVGDEDKIVRRGTPGEICVRTAMLFQEYV---------GNPEATKRAKTATGW 358

Query: 216 YRTGDFGILDK-GVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESS 392
           YR GD  ++D+ G I   GR    +K    ++   E++  V     V + VV+    +  
Sbjct: 359 YRLGDICVMDESGKIKVLGREKDFIKRATVKIFPAEIERVVLQHADVADVVVVGVPDQRF 418

Query: 393 NPEILAFVTI 422
           + EI A V +
Sbjct: 419 HEEICACVIL 428


>SB_16214| Best HMM Match : AMP-binding (HMM E-Value=0)
          Length = 549

 Score = 34.7 bits (76), Expect = 0.050
 Identities = 35/130 (26%), Positives = 55/130 (42%), Gaps = 3/130 (2%)
 Frame = +3

Query: 42  ISNNYVYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTH--PNFAKL 215
           +  N V ++ +  + VR G PGE+CV    L + YV          NP  T         
Sbjct: 360 LPGNEVKIVGDEDKIVRRGTPGEICVRTAMLFQEYV---------GNPEATKRAKTATGW 410

Query: 216 YRTGDFGILDK-GVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESS 392
           YR GD  ++D+ G I   GR    +K    ++   E++  V     V + VV+    +  
Sbjct: 411 YRLGDICVMDESGKIKVLGREKDFIKRATVKIFPAEIERVVLQHADVADVVVVGVPDQRF 470

Query: 393 NPEILAFVTI 422
           + EI A V +
Sbjct: 471 HEEICACVIL 480


>SB_57182| Best HMM Match : AMP-binding (HMM E-Value=0)
          Length = 566

 Score = 34.3 bits (75), Expect = 0.066
 Identities = 32/147 (21%), Positives = 65/147 (44%), Gaps = 1/147 (0%)
 Frame = +3

Query: 63  LLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFAKLYRTGDFGIL 242
           ++D+    V  GE GE+CV    +  GY+  ++  + + +P           R GD G++
Sbjct: 387 IIDDKGCLVPRGEMGEICVRNECVFMGYLGQEETTKKVKSPDG-------WIRMGDLGVM 439

Query: 243 D-KGVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEILAFVT 419
           D +G +   GR    +K    ++   E++ ++   P +   VV+    +    EI A V 
Sbjct: 440 DEEGRLQVLGRKVEVIKKASVKIVPGEIEASLMQHPLLARAVVIGVPDQVFYQEICACVE 499

Query: 420 INPEARMSAKNIEAELTSSLTDYMIPQ 500
            + +   ++  I  EL +   ++  P+
Sbjct: 500 FHGKGEDASTKI-MELDNWCIEHFPPR 525


>SB_39565| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 234

 Score = 34.3 bits (75), Expect = 0.066
 Identities = 30/121 (24%), Positives = 51/121 (42%), Gaps = 1/121 (0%)
 Frame = +3

Query: 57  VYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFAKLYRTGDFG 236
           V ++D N + V  GEPGE+C     +  GY+   +      +P            T D G
Sbjct: 52  VKIVDANNKVVPRGEPGEICCRSECVFLGYLGNLEATSRAKSPQG-------WLHTEDLG 104

Query: 237 IL-DKGVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEILAF 413
            + D+G+I   GR    +K    ++   E+   +   P V + +V+    +  + EI A 
Sbjct: 105 TMDDQGMIEVIGRTSEIIKRATVKIFPGEIKAELLKNPLVADAIVIGVPDQRLHEEICAC 164

Query: 414 V 416
           V
Sbjct: 165 V 165


>SB_36835| Best HMM Match : AMP-binding (HMM E-Value=6.2e-13)
          Length = 874

 Score = 32.7 bits (71), Expect = 0.20
 Identities = 27/95 (28%), Positives = 46/95 (48%), Gaps = 2/95 (2%)
 Frame = +3

Query: 30  IGVPISNNYVYLLD-ENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNF 206
           +GV + N    ++D  +   V +G+ GE+C+ G  + +GY+  K+P         T  + 
Sbjct: 206 VGVLLPNLECKIIDLSSGEEVEQGKEGEICIRGPTVMKGYL--KNPEA----TARTLDSE 259

Query: 207 AKLYRTGDFGILDKGVILY-AGRADSQVKIRGHRV 308
             L+ TGD G  D+G   Y   R    +K +G +V
Sbjct: 260 GWLH-TGDIGHCDQGDFFYITDRLKELIKYKGFQV 293


>SB_7062| Best HMM Match : AMP-binding (HMM E-Value=1.4e-18)
          Length = 817

 Score = 31.5 bits (68), Expect = 0.46
 Identities = 17/53 (32%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
 Frame = +3

Query: 216 YRTGDFGILDK-GVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVL 371
           Y TGD  + D+ G + + GR+D  +   G+R+   EV+ A+   P V    V+
Sbjct: 432 YLTGDRAVRDEDGYLWFIGRSDDVIISAGYRIGPFEVESALIEHPAVAEAAVV 484


>SB_19092| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 228

 Score = 30.7 bits (66), Expect = 0.81
 Identities = 28/127 (22%), Positives = 53/127 (41%), Gaps = 1/127 (0%)
 Frame = +3

Query: 57  VYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTHPNFAKLYRTGDFG 236
           V ++D + + V  G+PGE+C     +  GY+   +    + +P            T D G
Sbjct: 46  VKIVDTDGKVVPRGQPGEICCRSECVFLGYLGNPEATSRVKSPQG-------WLHTEDLG 98

Query: 237 IL-DKGVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEILAF 413
            + D+ +I   GR    +K    ++   E+   +   P V + +V+    +  + EI A 
Sbjct: 99  TMDDQDMIEVTGRKSEIIKRATVKIFPGEIRAELVKNPIVADTIVIGVPDKMLHEEICAC 158

Query: 414 VTINPEA 434
           V +   A
Sbjct: 159 VVLRKNA 165


>SB_57048| Best HMM Match : S-antigen (HMM E-Value=3.4)
          Length = 242

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = -3

Query: 372 TKRHNYPHTG**RQHDLPPAGQHGDPLS*PESQHDPHT 259
           T+  + PHT   R H  P   QH       ++QH PHT
Sbjct: 141 TRTRHEPHTDTTRTHTDPTQTQHRPNTDPTQTQHRPHT 178


>SB_19482| Best HMM Match : Pkinase_Tyr (HMM E-Value=2.4e-11)
          Length = 431

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 11/39 (28%), Positives = 25/39 (64%)
 Frame = +3

Query: 399 EILAFVTINPEARMSAKNIEAELTSSLTDYMIPQVIEIE 515
           E++     +P  R++ +N+E E+TS ++  + P+V +I+
Sbjct: 187 EVMRKCWADPNKRLTIRNVETEITSLMSQELGPRVHQIQ 225


>SB_57704| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 522

 Score = 28.7 bits (61), Expect = 3.3
 Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
 Frame = +3

Query: 3   QMHIFLTVPIGVPISNNYVYLLD-ENMRCVREGEPGEVCVAGFNLARGYV 149
           QM   +   +G P+    V L+D   M        GE+C+ G N+ +GY+
Sbjct: 133 QMRHGMLCHVGAPVPCATVKLVDVPEMDYYASNNQGEICIRGRNVFKGYL 182


>SB_10020| Best HMM Match : Extensin_2 (HMM E-Value=0.88)
          Length = 379

 Score = 28.7 bits (61), Expect = 3.3
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = -2

Query: 274 ARPAYKITPLSRMPKSPVRYSL 209
           ARP+Y   PLSR+P SP   SL
Sbjct: 314 ARPSYLYKPLSRIPTSPSLVSL 335


>SB_45441| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 111

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
 Frame = +3

Query: 30  IGVPISNNYVYLLD-ENMRCVREGEPGEVCVAGFNLARGY 146
           +G    N    ++D E+   V    PGE+C  G+N+ +GY
Sbjct: 52  VGTMAPNVEAKIIDSEHGNVVPINTPGEICFRGYNVMQGY 91


>SB_43771| Best HMM Match : AMP-binding (HMM E-Value=0.041)
          Length = 339

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
 Frame = +3

Query: 30  IGVPISNNYVYLLD-ENMRCVREGEPGEVCVAGFNLARGY 146
           +G    N    ++D E+   V    PGE+C  G+N+ +GY
Sbjct: 174 VGTMAPNVEAKIIDSEHGNVVPINTPGEICFRGYNVMQGY 213


>SB_13709| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 489

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 14/39 (35%), Positives = 19/39 (48%)
 Frame = -3

Query: 327 DLPPAGQHGDPLS*PESQHDPHTRSRLYPGCRNHQCDTV 211
           D PPA     P S P ++ +    S   PGC NH  D++
Sbjct: 87  DFPPASGGAVPWSRPPARFNFFFSSVHIPGCNNHIADSL 125


>SB_10665| Best HMM Match : PT (HMM E-Value=6.2)
          Length = 215

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 34/128 (26%), Positives = 46/128 (35%), Gaps = 7/128 (5%)
 Frame = -3

Query: 414 QTPESRDYCFPRRSTKRHNYPHTG**RQHDLPPAG-QHGDPLS*PESQHDPHTRSRLYPG 238
           +TP   D     R+   H+   T     HD  P   QHG   +  ++QH PHT     P 
Sbjct: 71  RTPHGHD-TDTTRTRHGHDTDTTRTPHGHDTDPTQTQHGHDTNPTQTQHGPHTNPTQTP- 128

Query: 237 CRNHQCDTVW----QN*GECYEGYG*SDADLYRAHIL*QD*NQPHTPHRVHL-PERIAYS 73
                 D  W     N       +G      +  H    D     TPHR H+ P R ++ 
Sbjct: 129 -HGPHTDPTWTPHRPNTDPTRTPHGPHTDPTWTPHRPHMD--PTRTPHRPHMDPTRTSHG 185

Query: 72  -HPEGKRN 52
            H +  RN
Sbjct: 186 PHTDPTRN 193


>SB_31957| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 538

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 31/140 (22%), Positives = 56/140 (40%), Gaps = 3/140 (2%)
 Frame = +3

Query: 57  VYLLDENMRCVREGEPGEVCVAGFNLARGYVRGKDPHRFIHNPHNTH--PNFAKLYRTGD 230
           V ++ +    V  G PGE+CV    +   YV          NP  T         +R GD
Sbjct: 354 VKIVGDGDEVVPRGTPGEICVRSAMVFSEYV---------GNPEATKQAKTATGWFRLGD 404

Query: 231 FGIL-DKGVILYAGRADSQVKIRGHRVDLQEVDHAVSTIPCVDNCVVLCYGVESSNPEIL 407
            G++ + G +   GR    +K    ++   +++  +     V + VV     +  + EI 
Sbjct: 405 IGVMNESGKVKVLGRKKDFIKRATLKISSLQIEKVILGHADVSDAVVFGVPDQQLHEEIC 464

Query: 408 AFVTINPEARMSAKNIEAEL 467
           A V +  +A  + + +E  L
Sbjct: 465 ACVIL--KAANNDRGLEVRL 482


>SB_42135| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 65

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = +3

Query: 174 IHNPHNTHPNFAKLYRTGDFGILDKGVILYAGRADSQVKIRGH 302
           I +PHN   +  K+  TG F + + G   Y  R  ++  IRGH
Sbjct: 7   IGSPHNKGSH--KVQNTGSFEVHNTGHTKYTIRGHTKYTIRGH 47


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,118,487
Number of Sequences: 59808
Number of extensions: 439264
Number of successful extensions: 1159
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1024
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1157
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1252112599
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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