BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_A10 (390 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, put... 52 1e-07 At1g56000.1 68414.m06425 amine oxidase-related contains Pfam pro... 31 0.21 At1g55980.1 68414.m06421 expressed protein 31 0.21 At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase NAD-bi... 30 0.48 At1g50080.1 68414.m05618 hypothetical protein 28 1.9 At3g52480.1 68416.m05771 expressed protein 27 4.5 At5g17700.1 68418.m02074 MATE efflux family protein similar to r... 27 5.9 At3g46340.1 68416.m05018 leucine-rich repeat protein kinase, put... 27 5.9 At2g27280.1 68415.m03278 hypothetical protein 27 5.9 At1g52990.1 68414.m05997 thioredoxin family protein similar to S... 27 5.9 At5g05970.1 68418.m00661 transducin family protein / WD-40 repea... 26 7.8 At4g23490.1 68417.m03384 fringe-related protein + weak similari... 26 7.8 At4g11350.1 68417.m01831 fringe-related protein various hypothet... 26 7.8 At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 26 7.8 At1g64320.1 68414.m07289 myosin heavy chain-related similar to m... 26 7.8 At1g12550.1 68414.m01455 oxidoreductase family protein similar t... 26 7.8 >At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, putative similar to S(+)-beta-hydroxybutyryl CoA dehydrogenase (3-hydroxybutyryl-CoA dehydrogenase) [Paracoccus denitrificans] GI:12003356; contains Pfam profiles PF02737: 3-hydroxyacyl-CoA dehydrogenase NAD binding, PF00725: 3-hydroxyacyl-CoA dehydrogenase C-terminal Length = 294 Score = 52.4 bits (120), Expect = 1e-07 Identities = 33/114 (28%), Positives = 59/114 (51%) Frame = +2 Query: 47 EKVGIVGSGLIGRSWAMLFASVGYQVTLFDVVEKQITDAIADIKVQLKTLEKDGLLRGNL 226 + VG+VG+G +G A L A+ G V L D ++ A A I +K GL+ + Sbjct: 5 KSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLISKEV 64 Query: 227 NADEQFQCVKGTCDLAIAVXDAIFVQECVPENLELKKKVFQNLDNVVDDNTILS 388 D+ ++ T +L + A + E + E+ ++KKK+F++LD + + IL+ Sbjct: 65 -GDDAMHRLRLTSNLE-DLCSADIIVEAIVESEDIKKKLFKDLDGIAKSSAILA 116 >At1g56000.1 68414.m06425 amine oxidase-related contains Pfam profile PF01593: amine oxidase, flavin-containing Length = 384 Score = 31.5 bits (68), Expect = 0.21 Identities = 16/41 (39%), Positives = 23/41 (56%) Frame = +2 Query: 14 LRVVMASKFKSEKVGIVGSGLIGRSWAMLFASVGYQVTLFD 136 +R M+S + KV ++GSG+ G A A G VT+FD Sbjct: 10 IRKQMSSVAVAMKVAVIGSGISGAVCASTLARNGVSVTIFD 50 >At1g55980.1 68414.m06421 expressed protein Length = 464 Score = 31.5 bits (68), Expect = 0.21 Identities = 16/41 (39%), Positives = 23/41 (56%) Frame = +2 Query: 14 LRVVMASKFKSEKVGIVGSGLIGRSWAMLFASVGYQVTLFD 136 +R M+S + KV ++GSG+ G A A G VT+FD Sbjct: 148 IRKQMSSVAVAMKVAVIGSGISGAVCASTLARNGVSVTIFD 188 >At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase NAD-binding domain-containing protein similar to SP|P23523 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60) (Tartronate semialdehyde reductase) {Escherichia coli}; contains Pfam profile PF03446: NAD binding domain of 6-phosphogluconate dehydrogenase Length = 334 Score = 30.3 bits (65), Expect = 0.48 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +2 Query: 50 KVGIVGSGLIGRSWAMLFASVGYQVTLFD 136 K+G +G+G++GRS GY VT+F+ Sbjct: 39 KIGWIGTGVMGRSMCGHLIKAGYTVTVFN 67 >At1g50080.1 68414.m05618 hypothetical protein Length = 91 Score = 28.3 bits (60), Expect = 1.9 Identities = 16/57 (28%), Positives = 24/57 (42%) Frame = -1 Query: 189 FNCTLISAIASVICFSTTSNKVTWYPTEANNIAHDLPISPLPTIPTFSDLNLEAMTT 19 FNC L+ AS I + + + NN+ H+ P PTI D + + T Sbjct: 18 FNCVLLCMFASFIRKLAAQSTIYNLWKQRNNVVHNQVSIPAPTIFKLIDREIRNIIT 74 >At3g52480.1 68416.m05771 expressed protein Length = 209 Score = 27.1 bits (57), Expect = 4.5 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -1 Query: 282 TAIAKSQVPFTHWNCSSAFRFPLRSPSF 199 T I+ +V F+H+N ++ F P SP F Sbjct: 159 TDISDDEVDFSHYNQTTPFSTPCASPPF 186 >At5g17700.1 68418.m02074 MATE efflux family protein similar to ripening regulated protein DDTFR18 [Lycopersicon esculentum] GI:12231296; contains Pfam profile PF01554: Uncharacterized membrane protein family Length = 497 Score = 26.6 bits (56), Expect = 5.9 Identities = 15/48 (31%), Positives = 27/48 (56%) Frame = +2 Query: 44 SEKVGIVGSGLIGRSWAMLFASVGYQVTLFDVVEKQITDAIADIKVQL 187 S KV +V S +IG + L + G Q++ + ++DA+AD+ + L Sbjct: 336 SIKVVLVVSAVIGVICSALCLAFGGQISYLFSDSQAVSDAVADLSIVL 383 >At3g46340.1 68416.m05018 leucine-rich repeat protein kinase, putative similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains leucine rich repeat (LRR) domains, Pfam:PF00560; contains protein kinase domain, Pfam:PF00069 Length = 889 Score = 26.6 bits (56), Expect = 5.9 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +2 Query: 197 EKDGLLRGNLNADEQFQCVKGTC 265 EKDGL L+ DEQ +C G+C Sbjct: 485 EKDGL---KLSVDEQIRCFPGSC 504 >At2g27280.1 68415.m03278 hypothetical protein Length = 427 Score = 26.6 bits (56), Expect = 5.9 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +2 Query: 134 DVVEKQITDAIADIKVQLKTLEK 202 D EK+ITDA+ K +L+ EK Sbjct: 41 DTAEKEITDAVTTAKERLRETEK 63 >At1g52990.1 68414.m05997 thioredoxin family protein similar to SP|P48384 Thioredoxin M-type, chloroplast precursor (TRX-M) {Pisum sativum}; contains Pfam profile PF00085: Thioredoxin Length = 313 Score = 26.6 bits (56), Expect = 5.9 Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 3/50 (6%) Frame = -1 Query: 354 KFWNTFFLSSKFSGTHSCTKIASXTAIAKSQVPFTHW---NCSSAFRFPL 214 K W F S++F TH + T + K+ T W NC F +P+ Sbjct: 143 KSWIPSFTSTEFIFTHREQNQCADTLVKKAIKSSTQWSLFNCCPHFLYPI 192 >At5g05970.1 68418.m00661 transducin family protein / WD-40 repeat family protein contains similarity to regulatory protein Nedd1; contains Pfam PF00400: WD domain, G-beta repeat (6 copies, 2 weak)|19804256|gb|AV785466.1|AV785466 Length = 781 Score = 26.2 bits (55), Expect = 7.8 Identities = 13/24 (54%), Positives = 15/24 (62%) Frame = +2 Query: 68 SGLIGRSWAMLFASVGYQVTLFDV 139 S L+ SW +L AS G V LFDV Sbjct: 3 SNLVEPSWRLLAASGGDTVKLFDV 26 >At4g23490.1 68417.m03384 fringe-related protein + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 526 Score = 26.2 bits (55), Expect = 7.8 Identities = 8/16 (50%), Positives = 12/16 (75%) Frame = +3 Query: 309 ASRKIWNSKRKYSKIW 356 AS K+W +++Y KIW Sbjct: 120 ASSKLWKQRKEYIKIW 135 >At4g11350.1 68417.m01831 fringe-related protein various hypothetical proteins from Arabidopsis thaliana strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 489 Score = 26.2 bits (55), Expect = 7.8 Identities = 8/16 (50%), Positives = 12/16 (75%) Frame = +3 Query: 309 ASRKIWNSKRKYSKIW 356 AS K+W +++Y KIW Sbjct: 98 ASSKLWKQRKEYIKIW 113 >At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent hydroxypyruvate reductase identical to hydroxypyruvate reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant Cell Physiol 1997 Apr;38(4):449-55) Length = 386 Score = 26.2 bits (55), Expect = 7.8 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 2/37 (5%) Frame = +2 Query: 41 KSEKVGIVGSGLIGRSWAMLFASVGYQVTL--FDVVE 145 K + VG++G+G IG ++A + G+++ L FD+ + Sbjct: 164 KGQTVGVIGAGRIGSAYARMMVE-GFKMNLIYFDLYQ 199 >At1g64320.1 68414.m07289 myosin heavy chain-related similar to myosin heavy chain (GI:4249703) [Rana catesbeiana]; similar to smooth muscle myosin heavy chain SM2 (GI:2352945) [Homo sapiens] Length = 476 Score = 26.2 bits (55), Expect = 7.8 Identities = 23/59 (38%), Positives = 29/59 (49%), Gaps = 10/59 (16%) Frame = +2 Query: 122 VTLFDVVEKQITDAIADIKVQLKTLEKD-GLLR-------GNLNAD--EQFQCVKGTCD 268 V F E +T I D+K QLK LE++ G LR GNL E+ + VKG D Sbjct: 38 VNKFGNSELGLTSRIEDLKCQLKNLEQEIGFLRARNAGLAGNLEVTKVEEKERVKGLMD 96 >At1g12550.1 68414.m01455 oxidoreductase family protein similar to glyoxylate reductase from Homo sapiens (gi:6002730); contains Pfam D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain PF02826 Length = 323 Score = 26.2 bits (55), Expect = 7.8 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = +2 Query: 26 MASKFKSEKVGIVGSGLIGRSWAMLFASVGYQVTLFDVVEKQ 151 + SK ++VGIVG G IG A S G ++ +KQ Sbjct: 146 LGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQ 187 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,775,692 Number of Sequences: 28952 Number of extensions: 171458 Number of successful extensions: 610 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 594 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 609 length of database: 12,070,560 effective HSP length: 73 effective length of database: 9,957,064 effective search space used: 557595584 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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