Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0007_H14
(640 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 177 1e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 110 2e-25
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 104 9e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 98 8e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 70 3e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 9e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 61 2e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 42 6e-05
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.11
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.14
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 1.7
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.0
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 4.0
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 4.0
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 25 7.0
SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr 1|||... 25 9.2
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 9.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 177 bits (431), Expect = 1e-45
Identities = 80/88 (90%), Positives = 84/88 (95%)
Frame = +1
Query: 334 LEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLS 513
+EKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVA QAVLS
Sbjct: 82 MEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLS 141
Query: 514 LYASGRTTGIVLDSGDGVSHTVPIYEGY 597
LYASGRTTGIVLDSGDGV+HTVPIYEGY
Sbjct: 142 LYASGRTTGIVLDSGDGVTHTVPIYEGY 169
Score = 168 bits (408), Expect = 7e-43
Identities = 74/83 (89%), Positives = 79/83 (95%)
Frame = +2
Query: 95 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 274
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 275 RGILTLKYPIEHGIVTNWDDWRR 343
RGILTLKYPIEHGIV NWDD +
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEK 84
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 110 bits (264), Expect = 2e-25
Identities = 46/86 (53%), Positives = 65/86 (75%)
Frame = +1
Query: 334 LEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLS 513
+ ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + +YVA QAVLS
Sbjct: 82 MNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLS 141
Query: 514 LYASGRTTGIVLDSGDGVSHTVPIYE 591
LYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 142 LYAQGLSSGVVVDSGDGVTHIVPVYE 167
Score = 75.8 bits (178), Expect = 5e-15
Identities = 37/80 (46%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
Frame = +2
Query: 101 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 274
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 275 RGILTLKYPIEHGIVTNWDD 334
R +L +KYP+E+GI+ ++++
Sbjct: 62 RSLLQVKYPMENGIIRDFEE 81
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 104 bits (250), Expect = 9e-24
Identities = 48/94 (51%), Positives = 69/94 (73%), Gaps = 2/94 (2%)
Frame = +1
Query: 334 LEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVL 510
+E+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N PA+ + Q VL
Sbjct: 86 MEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVL 145
Query: 511 SLYASGRTTGIVLDSGDGVSHTVPIYEGYG-PTA 609
+LYAS RTTGIVL+ GDG++H+VPIY+G+ P+A
Sbjct: 146 ALYASARTTGIVLECGDGLTHSVPIYDGFSIPSA 179
Score = 96.3 bits (229), Expect = 3e-21
Identities = 41/74 (55%), Positives = 54/74 (72%)
Frame = +2
Query: 113 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 292
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 293 KYPIEHGIVTNWDD 334
+ PIE GI+ NW D
Sbjct: 72 QRPIERGIIQNWSD 85
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 98.3 bits (234), Expect = 8e-22
Identities = 47/96 (48%), Positives = 62/96 (64%), Gaps = 8/96 (8%)
Frame = +1
Query: 334 LEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLS 513
+E+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN +Y+A QAVL+
Sbjct: 105 MERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLA 164
Query: 514 LYASGRT--------TGIVLDSGDGVSHTVPIYEGY 597
L AS + TG V+DSGDGV+H +P+ EGY
Sbjct: 165 LAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGY 200
Score = 48.4 bits (110), Expect = 9e-07
Identities = 33/101 (32%), Positives = 48/101 (47%), Gaps = 24/101 (23%)
Frame = +2
Query: 113 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 241
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 242 ------DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDWRR 343
D ++G++A K +L YPI HG + NWD R
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMER 107
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 69.7 bits (163), Expect = 3e-13
Identities = 32/85 (37%), Positives = 48/85 (56%)
Frame = +1
Query: 343 IWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYA 522
+W + +L+ P EHP+L+TE NP NR K + MFE+ PA Y+A Q + +A
Sbjct: 83 LWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFA 142
Query: 523 SGRTTGIVLDSGDGVSHTVPIYEGY 597
SG+ T ++D G S IY+G+
Sbjct: 143 SGKGTACLVDIGAERSSVSAIYDGF 167
Score = 44.0 bits (99), Expect = 2e-05
Identities = 27/78 (34%), Positives = 42/78 (53%)
Frame = +2
Query: 98 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 277
+EV+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 278 GILTLKYPIEHGIVTNWD 331
G + +K I +G V NWD
Sbjct: 62 G-MEIKNAIRNGWVENWD 78
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 61.7 bits (143), Expect = 9e-11
Identities = 29/89 (32%), Positives = 52/89 (58%), Gaps = 2/89 (2%)
Frame = +1
Query: 334 LEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLS 513
+E++ +TF +L + EHP+ +TE NP R MT+++FE +N P++ + S
Sbjct: 98 MEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFS 156
Query: 514 LYASGR--TTGIVLDSGDGVSHTVPIYEG 594
Y + + ++GIVL+ G+ SH +P+ G
Sbjct: 157 FYHNTKPSSSGIVLNLGNAASHVIPVLNG 185
Score = 46.4 bits (105), Expect = 3e-06
Identities = 23/74 (31%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = +2
Query: 113 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 292
LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR----KLSRTSTLVGNDTLIEVGSRSI 82
Query: 293 -KYPIEHGIVTNWD 331
+ P E +++NWD
Sbjct: 83 ARSPFERNVISNWD 96
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 60.9 bits (141), Expect = 2e-10
Identities = 24/83 (28%), Positives = 48/83 (57%)
Frame = +1
Query: 346 WHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQAVLSLYAS 525
W +L+V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+
Sbjct: 87 WERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFAN 146
Query: 526 GRTTGIVLDSGDGVSHTVPIYEG 594
++T +++D G + P+ +G
Sbjct: 147 SKSTALIVDIGSDNASVTPVVDG 169
Score = 43.6 bits (98), Expect = 2e-05
Identities = 25/80 (31%), Positives = 44/80 (55%), Gaps = 2/80 (2%)
Frame = +2
Query: 98 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 277
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 278 GI--LTLKYPIEHGIVTNWD 331
I + +K +GI+ +W+
Sbjct: 62 PIPGMEVKNGKSNGIIQDWE 81
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 42.3 bits (95), Expect = 6e-05
Identities = 28/91 (30%), Positives = 41/91 (45%), Gaps = 4/91 (4%)
Frame = +1
Query: 334 LEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAXQ 501
L+ W H Y+ L P + +PV L +RE TQ FE P +A +
Sbjct: 125 LKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFEECQVPGFTIAYE 183
Query: 502 AVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 594
++ LYA G G+V+D G + PI +G
Sbjct: 184 PLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.5 bits (68), Expect = 0.11
Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +1
Query: 358 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 480
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.14
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +2
Query: 158 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 301
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.5 bits (58), Expect = 1.7
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -2
Query: 255 PT*ESF*PM-PTMTPW*RGRPTIEGNTARGASSPAKPALHIPDPLSTTNAATSSS 94
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 400 LLTEAPLNPKANREKMTQIMFETFN 474
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 4.0
Identities = 13/42 (30%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = -3
Query: 560 SPESST-IPVVRPDAYSESTAWXATYMAGVLKVSNIICVIFS 438
+P+S+ + +V D S+S Y+A +K +N+IC+++S
Sbjct: 44 NPDSNDDVSLVLVDTQSDSNE--REYLAAEIKKANVICLVYS 83
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 26.2 bits (55), Expect = 4.0
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +2
Query: 353 TPSTMSCV*LPRSTQSCSPKLPSTPKPTE 439
TPS S P S+ + SP PSTP T+
Sbjct: 405 TPSKPSTFVRPHSSSTDSPPSPSTPSNTQ 433
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/31 (35%), Positives = 14/31 (45%)
Frame = +3
Query: 105 LPRWS*TMGPECAKPVSRETTLPAPYFPRSW 197
L W T P+C P +RET + SW
Sbjct: 481 LETWMKTTCPKCHGPATRETDTMDTFVDSSW 511
>SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr
1|||Manual
Length = 498
Score = 25.0 bits (52), Expect = 9.2
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = -1
Query: 394 GAPRELHAAHCRRCDARSSP 335
G PRE HCR C+ P
Sbjct: 52 GIPRESTVNHCRECERYMQP 71
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +2
Query: 218 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 304
+M+G +Y+G AQ KR +LT + I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,788,216
Number of Sequences: 5004
Number of extensions: 61685
Number of successful extensions: 181
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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