BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0006_O03
(539 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.8
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 26 4.1
SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces p... 25 5.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.2
SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 25 7.2
SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr ... 25 9.5
SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit Git... 25 9.5
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 27.1 bits (57), Expect = 1.8
Identities = 16/51 (31%), Positives = 23/51 (45%)
Frame = +3
Query: 126 SAVSSQXXXXXXXXXXXXPCTMNPSARTVRSSSRHSWRPSGGTSEDSSRLS 278
S+ SS P T + S+ + SSS S + S +S+ SSR S
Sbjct: 123 SSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSSRSS 173
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 25.8 bits (54), Expect = 4.1
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 10 RVFLSAPKKRKSFVIKKHSC 69
RVFL A ++R F++ HSC
Sbjct: 733 RVFLVANRERHPFLVNLHSC 752
>SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 832
Score = 25.4 bits (53), Expect = 5.4
Identities = 9/22 (40%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +2
Query: 263 LVKVKL-VPYGKSTHDKVNEKW 325
L++++L + Y S +D++NEKW
Sbjct: 776 LIRIELDIKYATSFYDELNEKW 797
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.0 bits (52), Expect = 7.2
Identities = 15/77 (19%), Positives = 30/77 (38%)
Frame = +3
Query: 126 SAVSSQXXXXXXXXXXXXPCTMNPSARTVRSSSRHSWRPSGGTSEDSSRLSWCLMERARM 305
S VSS + P++ + SSS S + S + S+ +S +
Sbjct: 370 STVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHNSTS 429
Query: 306 TR*MKSGLSRAITDLTN 356
+R S +++ L++
Sbjct: 430 SRSSSHSSSHSLSSLSS 446
>SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.0 bits (52), Expect = 7.2
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = +2
Query: 380 CILKDRSLQDTEKMELVICLMSQASPDKSLDTCLIQVNKQNE 505
CI+K +L+ +E + L++C S + LD L++V + +E
Sbjct: 23 CIVKFTNLRSSEDVNLLLCYAS-IHGEMYLDYVLVRVTEFDE 63
>SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 495
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 212 KKFITTQLAPVWRDFRGLVKVKLVPY 289
K+ ITT + VW FR L+ + VPY
Sbjct: 183 KESITTAVRWVWHAFRILLITRGVPY 208
>SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit
Git5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 305
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/39 (30%), Positives = 17/39 (43%), Gaps = 3/39 (7%)
Frame = +2
Query: 323 WTFTCHHGPDE---CYGNKMQACILKDRSLQDTEKMELV 430
W TC P G C++ D S+ D + +ELV
Sbjct: 65 WIMTCAFSPSTKSIAAGGLNNFCVVYDTSVPDADPVELV 103
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,113,528
Number of Sequences: 5004
Number of extensions: 40442
Number of successful extensions: 96
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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