Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0006_K15
         (611 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    57   2e-09
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    33   0.043
SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    29   0.53 
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    29   0.70 
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    27   2.8  
SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c...    26   4.9  
SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizo...    25   8.6  

>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1242

 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 27/79 (34%), Positives = 39/79 (49%)
 Frame = -3

Query: 339 NCIDDMCRICHGGERLSLEFGSLISACSCRGTIGRVHVKCLERWLTESGKSRCELCGTRY 160
           N  D++CR+C            L   C C G+I  VH +CL  WL  S K+ CELC  ++
Sbjct: 2   NADDEICRVCRCE---GAPDSPLFHPCKCTGSIRYVHQECLVEWLGHSKKTHCELCKAKF 58

Query: 159 STKRVHRYGVPRALVMWIL 103
              +V+   +PR +   IL
Sbjct: 59  EFTKVYSESMPRTIPFTIL 77



 Score = 25.8 bits (54), Expect = 4.9
 Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 3/35 (8%)
 Frame = +2

Query: 74  YVHTCL--AFWLRIHITKALGTPYLCTRL-VEYLV 169
           ++  CL  AF + + +T A+G PY+  RL VE+++
Sbjct: 450 FLQNCLVIAFVVSVFLTTAVGIPYMSGRLMVEWIL 484


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1610

 Score = 32.7 bits (71), Expect = 0.043
 Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
 Frame = -3

Query: 321  CRICHGGERLSLEFGSLISAC-SCRGTIGRVHVKCLERWLTESGKSRCELCGTRYS 157
            C IC+    LS+E       C +CR    + H  CL +W   S  SRC LC + ++
Sbjct: 1558 CAICYSV--LSVERTLPNKRCGTCRH---KFHASCLYKWFKSSNSSRCPLCRSSFT 1608


>SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 340

 Score = 29.1 bits (62), Expect = 0.53
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = -3

Query: 231 HVKCLERWLTESGKSRCELCGTRY 160
           H  CL  ++T SG +RC  C T Y
Sbjct: 44  HESCLISYITRSGSTRCPQCLTAY 67


>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 583

 Score = 28.7 bits (61), Expect = 0.70
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = -3

Query: 231 HVKCLERWLTESGKSRCELCGTR 163
           H +C+++WLT S  S C LC T+
Sbjct: 549 HRECIDQWLTSSQNS-CPLCRTK 570


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -3

Query: 231 HVKCLERWLTESGKSRCELC 172
           H  C+++WLT +G + C LC
Sbjct: 790 HQACIDQWLT-TGNNSCPLC 808


>SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 577

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 10/42 (23%), Positives = 23/42 (54%)
 Frame = +2

Query: 167 VPQSSHLDLPDSVSQRSRHFT*TLPIVPRHEHADIKDPNSSD 292
           +P+  HL++PD+V+ +++H        P  ++ +  +  S D
Sbjct: 476 LPELEHLEIPDNVALQNKHAIHITDCCPNLKYVNFSNSISLD 517


>SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 344

 Score = 25.0 bits (52), Expect = 8.6
 Identities = 10/32 (31%), Positives = 15/32 (46%)
 Frame = -3

Query: 231 HVKCLERWLTESGKSRCELCGTRYSTKRVHRY 136
           H    ER +++ G     +C  +YS  R H Y
Sbjct: 278 HASPFEREVSQGGSLALAVCNAQYSFPRKHPY 309


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,483,055
Number of Sequences: 5004
Number of extensions: 50463
Number of successful extensions: 132
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)