BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0006_D11
(611 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosacc... 160 2e-40
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 27 1.6
SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 25 6.5
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 8.6
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos... 25 8.6
SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor E|S... 25 8.6
>SPBP8B7.24c |atg8||autophagy associated protein Atg8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 160 bits (388), Expect = 2e-40
Identities = 68/116 (58%), Positives = 92/116 (79%)
Frame = +2
Query: 116 MKFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQF 295
M+ Q+K++ SFEKRKTE ++IR KYPDR+PVI EK K+ + +DKKKYLVPSDLTVGQF
Sbjct: 1 MRSQFKDDFSFEKRKTESQRIREKYPDRIPVICEKVDKSDIAAIDKKKYLVPSDLTVGQF 60
Query: 296 YFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDKDFFLYIAFSDENVYG 463
++IRKRI L PE A+F F++ ++PPT+A M ++Y+EH +D FLYI +S EN +G
Sbjct: 61 VYVIRKRIKLSPEKAIFIFIDEILPPTAALMSTIYEEHKSEDGFLYITYSGENTFG 116
>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 27.5 bits (58), Expect = 1.6
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = -1
Query: 467 NIHKHFHQK--MLCRERNPCRDAPGRENP*LQMLVESHCSRKRKARPRDE 324
N H+ +K +L RERN C L++L +++ KR+AR +E
Sbjct: 291 NAHQLLEKKFEILKRERNECNAKIDELQDKLELLTDAYNREKRRARSLEE 340
>SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1125
Score = 25.4 bits (53), Expect = 6.5
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -3
Query: 216 STITGTRSGYFLRIFSPSVFLFSKECSSLYWNFIFVF 106
S + R+G+ + + +++F EC Y N I +F
Sbjct: 163 SPLPSPRTGHSMLLVDSKLWIFGGECQGKYLNDIHLF 199
>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 314
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 107 KTKMKFQYKEEHSFEKRKTEGEKIRRKYPDRVP 205
K M+ + K+ SFEKR +++ K P R P
Sbjct: 63 KETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTP 95
>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 449
Score = 25.0 bits (52), Expect = 8.6
Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Frame = -2
Query: 511 HLKYNKSNI*NFK----ELISINIFIRKCYVEKEILVVMLLVERTHSCRCWWNH 362
HLK +++ N++ ++ S + R+C K I + HS C W H
Sbjct: 10 HLKLKPADVENYQKICTQIFSCHFVPRRCSTCKRINKRSIRCLSCHSVGCLWGH 63
>SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor
E|Schizosaccharomyces pombe|chr 1|||Manual
Length = 511
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 103 LRNNLTKKLLFSSTQSTATCFLFLRYS 23
L+N +TK + SS S AT L L Y+
Sbjct: 419 LKNRITKGIKISSINSGATMLLKLHYN 445
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,287,752
Number of Sequences: 5004
Number of extensions: 47395
Number of successful extensions: 123
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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