Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0006_B02
(473 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 208 3e-55
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 119 2e-28
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 116 1e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 102 3e-23
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.1
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 27 1.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 1.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 4.5
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 4.5
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 4.5
SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit S19... 25 7.8
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 7.8
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 25 7.8
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 208 bits (508), Expect = 3e-55
Identities = 88/120 (73%), Positives = 105/120 (87%)
Frame = +2
Query: 2 QIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMD 181
Q+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYVPRA+LVDLEPGTMD
Sbjct: 15 QVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMD 74
Query: 182 SVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQ 361
+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+CD LQGFQ
Sbjct: 75 AVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQ 134
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 119 bits (287), Expect = 2e-28
Identities = 54/121 (44%), Positives = 77/121 (63%), Gaps = 2/121 (1%)
Frame = +2
Query: 2 QIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGKYVPRAILVDLEPGT 175
QIG WE+ EHGI P G + ++ Q + +++E GKYVPR+I VDLEP
Sbjct: 15 QIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNV 74
Query: 176 MDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQG 355
+D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C LQG
Sbjct: 75 IDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQG 134
Query: 356 F 358
F
Sbjct: 135 F 135
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 116 bits (280), Expect = 1e-27
Identities = 53/125 (42%), Positives = 78/125 (62%), Gaps = 6/125 (4%)
Frame = +2
Query: 2 QIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEASGGKYVPRAILVDL 163
QIG WE+ EHGI P G H ++ + +++E GK+VPR+I VDL
Sbjct: 15 QIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDL 74
Query: 164 EPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCD 343
EP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 75 EPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCS 134
Query: 344 CLQGF 358
LQGF
Sbjct: 135 GLQGF 139
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 102 bits (244), Expect = 3e-23
Identities = 42/121 (34%), Positives = 79/121 (65%), Gaps = 2/121 (1%)
Frame = +2
Query: 2 QIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMD 181
QIG++FW+ + EHGI P G + ++R +V++ ++ +Y+PRAIL+DLEP ++
Sbjct: 16 QIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVN 75
Query: 182 SVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQG 355
++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E++ D L+G
Sbjct: 76 NILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEG 134
Query: 356 F 358
F
Sbjct: 135 F 135
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 263 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 352
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 26.6 bits (56), Expect = 1.9
Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 9/91 (9%)
Frame = +3
Query: 189 VRDLSDRYSALTTLFSDSPAPVTTGPRDTTQR-ALS*STQFLT*SEKNPNHVIVYKDSN- 362
+ D +R+ L F SP+ +T P+ + LS S +++ S ++P+H V S+
Sbjct: 27 ISDSPNRHHNLVDAFMQSPS-YSTQPKSAVEPLGLSFSPGYISPSSQSPHHGPVRSPSSR 85
Query: 363 -------SHTLSEAVPAPVWAHYLSQRSNPR 434
S T ++ PV H S PR
Sbjct: 86 KPLPASPSRTRDHSLRVPVSGHSYSADEKPR 116
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 1.9
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 194 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 295
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 4.5
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 215 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 346
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 4.5
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 252 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 157
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.4 bits (53), Expect = 4.5
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = +3
Query: 330 PNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSNPRGQS 443
P VIV +DS S L+ +P P H L P+ S
Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQLQSFVPPQPPS 60
>SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit
S19|Schizosaccharomyces pombe|chr 1|||Manual
Length = 108
Score = 24.6 bits (51), Expect = 7.8
Identities = 15/54 (27%), Positives = 22/54 (40%)
Frame = +3
Query: 249 PVTTGPRDTTQRALS*STQFLT*SEKNPNHVIVYKDSNSHTLSEAVPAPVWAHY 410
P+ T R T QF+ + K+ +V + +D H L E P HY
Sbjct: 49 PIKTAVRSATILPRMVGAQFMVHNGKSYANVKITEDMIGHKLGEFAPTRKAFHY 102
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 24.6 bits (51), Expect = 7.8
Identities = 10/37 (27%), Positives = 16/37 (43%)
Frame = +3
Query: 321 EKNPNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSNP 431
+ N NH ++ S SH+L + W H + P
Sbjct: 901 QNNDNHCVLCLQSASHSLMKKTVEGNWVHLICASWTP 937
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 24.6 bits (51), Expect = 7.8
Identities = 9/33 (27%), Positives = 21/33 (63%)
Frame = +2
Query: 2 QIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 100
++G E+++++ +DPT A + DLQ++ +
Sbjct: 134 ELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 24.6 bits (51), Expect = 7.8
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 219 GRNICPKGPERTESMVPGSRSTRMARGTYLP 127
G NI PER + +S RMAR LP
Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELP 136
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,752,799
Number of Sequences: 5004
Number of extensions: 32350
Number of successful extensions: 107
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 182448900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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