BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_L03
(424 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 94 7e-21
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 75 6e-15
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 72 4e-14
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 71 6e-14
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 63 2e-11
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 56 3e-09
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 43 2e-05
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.018
SPBC3D6.06c |||ribose-phosphate pyrophosphokinase |Schizosacchar... 29 0.39
SPCC16A11.05c |dim1||U4/U6 x U5 tri-snRNP complex subunit Dim1|S... 28 0.69
SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual 25 4.8
SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 25 4.8
SPBC31E1.02c |pmr1||P-type ATPase, calcium transporting Pmr1 |Sc... 25 4.8
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 6.4
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 25 6.4
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 25 6.4
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 24 8.5
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 94.3 bits (224), Expect = 7e-21
Identities = 45/98 (45%), Positives = 65/98 (66%)
Frame = +1
Query: 118 IKDFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDVDECE 297
+ D + K+ + + DKLVV+DF ATWCGPCK I PK ++ +N SD+ +KVDVD+
Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDA-TFIKVDVDQLS 61
Query: 298 DIAAEYNINAMPTFVFVKATKKLEEFSGANVDXLKNTI 411
+IAAE ++AMP+F K +K+EE GAN L+ +I
Sbjct: 62 EIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAKLEASI 99
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 74.5 bits (175), Expect = 6e-15
Identities = 35/100 (35%), Positives = 59/100 (59%)
Frame = +1
Query: 118 IKDFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDVDECE 297
++ F D TR+S DK+ V+DF A WCGPCK + P L+++ +E + + V+ D+
Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78
Query: 298 DIAAEYNINAMPTFVFVKATKKLEEFSGANVDXLKNTILK 417
DIA + + A+PT V + ++L+ GA+V L + + K
Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 71.7 bits (168), Expect = 4e-14
Identities = 36/101 (35%), Positives = 57/101 (56%), Gaps = 1/101 (0%)
Frame = +1
Query: 112 IHIKDFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEM-SDSIVVLKVDVD 288
I I+ + + + ++G + +D A WCGPCK I P ++A++ S V KV+VD
Sbjct: 4 IEIRSYQHWISTIPKSG--YLAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVD 61
Query: 289 ECEDIAAEYNINAMPTFVFVKATKKLEEFSGANVDXLKNTI 411
E IA+ + AMPTFVF + K+++ +GAN LK +
Sbjct: 62 EQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKV 102
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 71.3 bits (167), Expect = 6e-14
Identities = 32/102 (31%), Positives = 56/102 (54%)
Frame = +1
Query: 106 MSIHIKDFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDV 285
MS+ I + + L +++++++F A W PCK + D+ A + ++ V LK++
Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNA-VFLKIEA 59
Query: 286 DECEDIAAEYNINAMPTFVFVKATKKLEEFSGANVDXLKNTI 411
++ DIA +++NA+P FV + K L SGAN LK I
Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGANPQKLKAAI 101
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 62.9 bits (146), Expect = 2e-11
Identities = 27/74 (36%), Positives = 46/74 (62%), Gaps = 1/74 (1%)
Frame = +1
Query: 163 DKLVVIDFMATWCGPCKMIGPKLDEMANEM-SDSIVVLKVDVDECEDIAAEYNINAMPTF 339
DK++++ F A WCG CK + P+ + A+E+ D I +++VD E D+ +EY+I PT
Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98
Query: 340 VFVKATKKLEEFSG 381
K K++ ++SG
Sbjct: 99 NVFKNGKQISQYSG 112
Score = 56.0 bits (129), Expect = 2e-09
Identities = 32/83 (38%), Positives = 50/83 (60%), Gaps = 2/83 (2%)
Frame = +1
Query: 124 DFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSD--SIVVLKVDVDECE 297
+FDD+ + E D V+++F A WCG CK + P +++A E SD ++VV K+D E
Sbjct: 364 NFDDIV--MDETKD--VLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATE-N 418
Query: 298 DIAAEYNINAMPTFVFVKATKKL 366
DI+ +I+ PT +F KA K+
Sbjct: 419 DISV--SISGFPTIMFFKANDKV 439
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 55.6 bits (128), Expect = 3e-09
Identities = 24/80 (30%), Positives = 45/80 (56%), Gaps = 2/80 (2%)
Frame = +1
Query: 112 IHIKDFDDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSD--SIVVLKVDV 285
+ ++ ++L+ + A K +I+F ATWCG CK + P +E+ D +++ K+D
Sbjct: 23 VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDA 81
Query: 286 DECEDIAAEYNINAMPTFVF 345
D D+A +Y+I PT ++
Sbjct: 82 DTHSDVADKYHITGFPTLIW 101
Score = 41.5 bits (93), Expect = 5e-05
Identities = 17/62 (27%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
Frame = +1
Query: 166 KLVVIDFMATWCGPCKMIGPKLDEMANEMSD--SIVVLKVDVDECEDIAAEYNINAMPTF 339
K V+++F A WCG CK + P + + + ++ ++K++ D DI + + + PT
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTI 218
Query: 340 VF 345
F
Sbjct: 219 KF 220
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 42.7 bits (96), Expect = 2e-05
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 2/78 (2%)
Frame = +1
Query: 160 GDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDVDECED--IAAEYNINAMP 333
G LVV F A WCG CK + P ++A+ + + V VD D ++ + ++Y + P
Sbjct: 49 GPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFP 106
Query: 334 TFVFVKATKKLEEFSGAN 387
T V + K S +
Sbjct: 107 TIKLVYPSSKGSSLSSTD 124
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 33.1 bits (72), Expect = 0.018
Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +1
Query: 151 SEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKV---DVDECEDIAAEYNI 321
SE I + CG CK +GP D M + + + +VD +++++ NI
Sbjct: 38 SEVSKGTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANI 97
Query: 322 NAMPTFVFVKATKKLEE 372
A+PT + + +EE
Sbjct: 98 RAVPTLYLYQNGEIVEE 114
Score = 32.3 bits (70), Expect = 0.032
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = +1
Query: 178 IDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDVDECEDIAAEYNINAMPTFVFVK 351
I F ++ C C + MAN M + V ++ + +Y+I PTF+F K
Sbjct: 303 IQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQYFPTFLFFK 360
>SPBC3D6.06c |||ribose-phosphate pyrophosphokinase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 341
Score = 28.7 bits (61), Expect = 0.39
Identities = 12/61 (19%), Positives = 32/61 (52%)
Frame = +1
Query: 241 ANEMSDSIVVLKVDVDECEDIAAEYNINAMPTFVFVKATKKLEEFSGANVDXLKNTILKL 420
+ +++D ++ L + + C+ +A+ +P F + + + FSGA + L++ ++
Sbjct: 60 SGQVNDHLMELLIMISACKTASAKKVTAVLPVFPYSRQPDQKFSFSGAPLSDLQDAVVPC 119
Query: 421 K 423
K
Sbjct: 120 K 120
>SPCC16A11.05c |dim1||U4/U6 x U5 tri-snRNP complex subunit
Dim1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 142
Score = 27.9 bits (59), Expect = 0.69
Identities = 21/73 (28%), Positives = 32/73 (43%)
Frame = +1
Query: 148 LSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIVVLKVDVDECEDIAAEYNINA 327
LSE ++LVVI F C L +A ++ + V+ VD+DE D Y +
Sbjct: 19 LSEQ-ERLVVIRFGRDHDEECIKQDEVLYRIAEKVVNMAVIYLVDIDEVPDFNKMYELYD 77
Query: 328 MPTFVFVKATKKL 366
T +F K +
Sbjct: 78 RTTIMFFYRNKHM 90
>SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 394
Score = 25.0 bits (52), Expect = 4.8
Identities = 13/38 (34%), Positives = 15/38 (39%)
Frame = -2
Query: 252 HFVSHLVEFGPDHLAGSAPSRHEVNHDELVARLRQPGL 139
HF SHL + P G S +NH Q GL
Sbjct: 207 HFDSHLDSWKPKVFGGGKSSVGSINHGTYFYHASQEGL 244
>SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.0 bits (52), Expect = 4.8
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = +1
Query: 139 KTRLSEAGDKLVVI---DFMATWCGPCKMIGPKLDEMANEMSDSIVVL 273
K +EAG I D ++ W G + + +L EMA E SI+ +
Sbjct: 180 KAVATEAGSTFFSISSSDLVSKWMGESERLVRQLFEMAREQKPSIIFI 227
>SPBC31E1.02c |pmr1||P-type ATPase, calcium transporting Pmr1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 899
Score = 25.0 bits (52), Expect = 4.8
Identities = 12/62 (19%), Positives = 29/62 (46%)
Frame = -2
Query: 411 DGVLEXVDVRAGELFEFLGRLNKHESRHRVDVIFGGDVFAFVNVDLKNNNGVGHFVSHLV 232
DGV + ++ ++ +GR ++ D+I D FA + ++ G+ + + + +
Sbjct: 627 DGVNDAPALKLADIGIAMGRQGTDVAKEAADMILTDDSFATILSAVEEGKGIFNNIKNFI 686
Query: 231 EF 226
F
Sbjct: 687 TF 688
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 24.6 bits (51), Expect = 6.4
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +1
Query: 130 DDLKTRLSEAGDKLVVIDFMATWCGPCKMIGPKLDEMANEMSDSIV 267
D + RL EAG + +A CGPC + + D++A+ ++I+
Sbjct: 433 DGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAIL 473
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 24.6 bits (51), Expect = 6.4
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +1
Query: 286 DECEDIAAEYNINAMPTFVFVKATKKL 366
D+CE+ AE+ F+F+K ++L
Sbjct: 518 DQCEEFVAEFRQTEEKYFLFLKRLREL 544
>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 366
Score = 24.6 bits (51), Expect = 6.4
Identities = 8/41 (19%), Positives = 23/41 (56%)
Frame = +1
Query: 205 PCKMIGPKLDEMANEMSDSIVVLKVDVDECEDIAAEYNINA 327
P ++ K + +++++ + +D+DE + +EY +N+
Sbjct: 175 PPNIVFKKKERGGINITNTVPLTHIDLDEIRAVCSEYRVNS 215
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 24.2 bits (50), Expect = 8.5
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 352 ATKKLEEFSGANVDXLKNTILKLK 423
A+KKL E G D L+N + K+K
Sbjct: 162 ASKKLNEQDGKKSDNLRNGVNKVK 185
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,529,074
Number of Sequences: 5004
Number of extensions: 28492
Number of successful extensions: 142
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 150383836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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