Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_I21
(642 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 44 2e-05
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 40 4e-04
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 39 5e-04
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 39 7e-04
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 37 0.003
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo... 29 0.75
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 27 1.7
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 27 3.0
SPAC6G9.05 |pcd1||coenzyme A diphosphatase |Schizosaccharomyces ... 26 4.0
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 26 5.3
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 25 7.0
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 7.0
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 25 7.0
SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosac... 25 9.3
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 44.0 bits (99), Expect = 2e-05
Identities = 32/116 (27%), Positives = 52/116 (44%)
Frame = +1
Query: 238 QPTNLDPENTIALLISTSGTTGLPKSAILTHKNLSVAVPYLWLNFSNFPTPTKMAMIVSP 417
QP L + + L++ TSGTTG PK LTHKNL ++ + ++ P T + +V P
Sbjct: 153 QPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNLCRSIHNITTSYRLDPRDT--SYVVMP 210
Query: 418 LQWMSAIFHFLFSPVLKYPRVQSSETITQDIFYKIVNKYKPSHLIISPTLMAYLLK 585
L + + L S + + F+K +Y + PT+ LL+
Sbjct: 211 LFHVHGLLCGLLSTLASGGCAVVPPKFSAHSFWKEFIQYGATWYTAVPTIHQILLR 266
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 39.5 bits (88), Expect = 4e-04
Identities = 24/57 (42%), Positives = 35/57 (61%)
Frame = +1
Query: 181 KIITFDRRSEFQDISDFIEQPTNLDPENTIALLISTSGTTGLPKSAILTHKNLSVAV 351
K+I F ++F+ +S +E P PE I ++ TSG+TGLPK IL+HKN+ V
Sbjct: 217 KLIKF---ADFEKMSPPVE-PDPPSPEE-ICCIMYTSGSTGLPKGVILSHKNMVAIV 268
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 39.1 bits (87), Expect = 5e-04
Identities = 36/145 (24%), Positives = 57/145 (39%)
Frame = +1
Query: 37 AKVAILDPTLGVNELKDLFEVNEPKFIFCQSEKASVAHSALLNTDLKSKIITFDRRSEFQ 216
++ DP L L L + K I C S + + +T +IIT+D
Sbjct: 173 SRAIFTDPNLIPKLLGPLKQSTWVKLIVCSSTPSEDLVELVKSTAPDVEIITYDN---LL 229
Query: 217 DISDFIEQPTNLDPENTIALLISTSGTTGLPKSAILTHKNLSVAVPYLWLNFSNFPTPTK 396
+ QP + + I + TSG+TG PK +L H+N+ A+ + S
Sbjct: 230 SLGKEKPQPPHPPKADDICCYMYTSGSTGKPKGVVLLHRNIIAALGGINRILSQHINVKD 289
Query: 397 MAMIVSPLQWMSAIFHFLFSPVLKY 471
+ PL + IF F+F Y
Sbjct: 290 YVLAYLPL---AHIFEFIFEMCCLY 311
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 38.7 bits (86), Expect = 7e-04
Identities = 33/119 (27%), Positives = 59/119 (49%), Gaps = 7/119 (5%)
Frame = +1
Query: 250 LDPENTIALLISTSGTTGLPKSAILTHKNLSVAVPYLWLNFSN-FPTPTKMA--MIVSPL 420
L+PE +AL+ +SG+TG+PKS K ++ Y F N TP MA ++ L
Sbjct: 158 LEPE--VALINHSSGSTGVPKSMPFLMKKYALGYDYGTPEFMNHLSTPMVMATSFALTTL 215
Query: 421 QWMSAIF---HFLF-SPVLKYPRVQSSETITQDIFYKIVNKYKPSHLIISPTLMAYLLK 585
W++A++ + L+ S + +E + +++Y + + LII P L+ L+
Sbjct: 216 SWINALYCNGNLLYPSSSISATCTSEAERLAWNVYYAL--RAGCERLIILPNLLVLTLQ 272
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 36.7 bits (81), Expect = 0.003
Identities = 23/65 (35%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
Frame = +1
Query: 148 HSALLNTDLKSKIITFDRRSEFQDISDFIEQPTNLDPENT-IALLISTSGTTGLPKSAIL 324
++ LLN + I+ S+ + S+ IE P D E++ +A ++ TSG+TG PK L
Sbjct: 2834 YTKLLNASINVTILD---ASDPGNYSNNIENPYTKDFEDSNLAYVLYTSGSTGKPKGCCL 2890
Query: 325 THKNL 339
TH N+
Sbjct: 2891 THHNV 2895
Score = 27.5 bits (58), Expect = 1.7
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +1
Query: 241 PTNLDPENTIALLISTSGTTGLPKSAILTHK 333
P +LD ++A ++ TSG+TG PK ++H+
Sbjct: 355 PESLD---SVAYVLYTSGSTGNPKGVAISHR 382
>SPAC4F10.09c |||ribosome biogenesis protein Noc1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 28.7 bits (61), Expect = 0.75
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = +1
Query: 67 GVNELKDLFEVNEPKFIFCQSEKASVAHSALLNTDLKSKIITFDRRSEFQDISD 228
GVN EVN KF + ++ H+A NT ++ ++ F + ISD
Sbjct: 376 GVNRAYPFAEVNSEKFDKHMNTLFAITHTASFNTSVQVLMLIFQASASRDFISD 429
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 27.5 bits (58), Expect = 1.7
Identities = 33/136 (24%), Positives = 60/136 (44%), Gaps = 6/136 (4%)
Frame = +1
Query: 241 PTNLDPENTIALLISTSGTTGLPKSAI-LTHKNLSVAVPYLWLNFSNFPTPTKMAMIVSP 417
P ++PE+ + LL TSG+TG PK + T L A F PT +M
Sbjct: 259 PAVVNPEHPLFLLY-TSGSTGKPKGVVHCTGGYLLGAAATCKYVFDLHPT-DRMG-CAGD 315
Query: 418 LQWMSAIFHFLFSPVL--KYPRVQSSETITQDI--FYKIVNKYKPSHLIISPTLMAYLLK 585
+ W++ + ++ P++ V S D ++ +V +++ + I+PT + L +
Sbjct: 316 VGWITGHTYIVYGPLMLGAATLVFESTPAYPDYSRYWSVVERHRLTQWYIAPTAIRLLQR 375
Query: 586 Q-NSIVASAISSFQTI 630
N V SS + +
Sbjct: 376 AGNEFVKHDRSSLRVL 391
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 26.6 bits (56), Expect = 3.0
Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 5/96 (5%)
Frame = +1
Query: 94 EVNEPKFIFCQSEKASVAHSALLNTDLKSKIITFDRRSEFQDISDF-IEQPTN----LDP 258
EVNE K ++ S + LKS+ T + SE D SDF + P+N + P
Sbjct: 35 EVNELKPAKSKAFSISAQDQIYGTSSLKSRFSTHEASSEKDDSSDFTLVLPSNRMYIVIP 94
Query: 259 ENTIALLISTSGTTGLPKSAILTHKNLSVAVPYLWL 366
+++ +S T + + NL Y W+
Sbjct: 95 GLMLSIFLSALDQTVITTAIPTIVANLDGGSSYSWI 130
>SPAC6G9.05 |pcd1||coenzyme A diphosphatase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 285
Score = 26.2 bits (55), Expect = 4.0
Identities = 18/49 (36%), Positives = 24/49 (48%)
Frame = +1
Query: 28 FLGAKVAILDPTLGVNELKDLFEVNEPKFIFCQSEKASVAHSALLNTDL 174
+L V P+LG E+KDLF V F+ + +K S NTDL
Sbjct: 202 YLAFSVQTSLPSLGTGEVKDLFYVPLTSFLNPKHQKI----SRFRNTDL 246
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 25.8 bits (54), Expect = 5.3
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +1
Query: 220 ISDFIEQPTNLDPENTI 270
+SDF+ P +DP NTI
Sbjct: 588 VSDFLNDPDEVDPVNTI 604
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 25.4 bits (53), Expect = 7.0
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -2
Query: 455 ENKK*NIADIHCN 417
ENKK I DIHCN
Sbjct: 72 ENKKNTIPDIHCN 84
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.4 bits (53), Expect = 7.0
Identities = 29/77 (37%), Positives = 37/77 (48%), Gaps = 8/77 (10%)
Frame = +1
Query: 259 ENTIALLISTSGTTGLPKSAILTHKNLSV---AVPYLW--LN-FSNF--PTPTKMAMIVS 414
E L +STS +T L KSA + N+S + P LW LN FS F + + IV
Sbjct: 2277 EEVNRLAVSTSNSTYLLKSASAVYHNVSSFKGSTPSLWNLLNQFSKFLIEIASANSNIVY 2336
Query: 415 PLQWMSAIFHFLFSPVL 465
L + I HFL VL
Sbjct: 2337 KLSY-DVIRHFLKLVVL 2352
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 160 LNTDLKSKIITFDRRSEFQDISDFIEQP 243
L+ ++K I+ FDR F ++S +EQP
Sbjct: 327 LDPNVKIAIVCFDRSVNFFNLSPNLEQP 354
>SPAC1751.01c |gti1||gluconate transporter inducer
Gti1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 25.0 bits (52), Expect = 9.3
Identities = 30/130 (23%), Positives = 54/130 (41%), Gaps = 2/130 (1%)
Frame = +1
Query: 178 SKIITFDRRS--EFQDISDFIEQPTNLDPENTIALLISTSGTTGLPKSAILTHKNLSVAV 351
S++ T ++R+ F F +DP + L +STS +G+ S +LS A+
Sbjct: 353 SQLSTLEKRTLPSFPYNPFFSSSTAEIDPYHFQGLTLSTSPISGVESS------SLSSAI 406
Query: 352 PYLWLNFSNFPTPTKMAMIVSPLQWMSAIFHFLFSPVLKYPRVQSSETITQDIFYKIVNK 531
N S+F + + + S +A+ + P +K S Q + + +
Sbjct: 407 SRNQSNLSSFQQQQQFSALQSISN--NALNENIEQPPIKMAARHSYPNFLQTLPQYMNDV 464
Query: 532 YKPSHLIISP 561
Y PS L +P
Sbjct: 465 YNPSGLAFNP 474
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,712,953
Number of Sequences: 5004
Number of extensions: 57171
Number of successful extensions: 164
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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