Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_E18
(518 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.32
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 1.7
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 26 2.9
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 2.9
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 3.9
SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces pomb... 26 3.9
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 6.8
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 9.0
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 9.0
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 25 9.0
SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosacchar... 25 9.0
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 9.0
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 29.5 bits (63), Expect = 0.32
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 3/32 (9%)
Frame = -1
Query: 158 KDRGSAVRKSVQCS---PPLWGYVQVYNSQMP 72
+ +G ++ KS CS PPL G+V V+N Q P
Sbjct: 254 RSKGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 27.1 bits (57), Expect = 1.7
Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 335 VQGLLSYPEICAKLINPNQNGKRPHLRKVNDP-SKRFGTYAFRLPDDHGEGGFWVSYEDP 511
V L+SYP++ ++ + + +LR++N P + F +A P E GF+V D
Sbjct: 1815 VNSLISYPKMNIEVSQSSSDKLLLYLRRLNQPLNPDFYIFAPLFPKPQSE-GFFVLIIDS 1873
Query: 512 DT 517
+T
Sbjct: 1874 ET 1875
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.2 bits (55), Expect = 2.9
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +2
Query: 293 IHADEGGEAGPYTKVQGLLSY 355
+H + G AG Y KV+G + Y
Sbjct: 562 LHKKQSGGAGQYAKVEGYIEY 582
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 26.2 bits (55), Expect = 2.9
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +3
Query: 210 PTNLSL-VSAPLDVRGSSIQTVKFPEFHRYT 299
PT L SA DVR +S + +FH+Y+
Sbjct: 51 PTELKARTSATFDVRSASTSPINASDFHKYS 81
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 3.9
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2
Query: 128 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWK--LDSDSEIS 280
P PQ+ + +QN + + + G P K V+ + T DSDS++S
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSDSKLS 829
>SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 730
Score = 25.8 bits (54), Expect = 3.9
Identities = 19/83 (22%), Positives = 33/83 (39%), Gaps = 5/83 (6%)
Frame = +2
Query: 257 LDSDSEISGVPPIHADEGGEAGPYTKVQGLLSYPEICAKLINPNQNGKR-----PHLRKV 421
L S+++ + D P T + +P + K ++ +NG P+L+
Sbjct: 464 LSSNNKPQEYVGVENDYNFSNNPPTAMSEQSFHPSVSQKPMDTPENGSNSFAASPYLQPY 523
Query: 422 NDPSKRFGTYAFRLPDDHGEGGF 490
N S+ +Y LP HG F
Sbjct: 524 NSASQLAPSYVGSLPQYHGNPSF 546
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +2
Query: 23 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 118
Y D P I+ V +N+ D T + NP + D
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +3
Query: 195 KMVRLPTNLSLVSAPLDVR---GSSIQTVKFPEFHRYTP 302
+++ +P NL+ PL GSS +T + PEF TP
Sbjct: 144 ELIMVPPNLA--GKPLSANSSFGSSAKTFQLPEFDSVTP 180
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 24.6 bits (51), Expect = 9.0
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 95 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 214
+R+P + Y + P N +NA A+NY+ + A H
Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 24.6 bits (51), Expect = 9.0
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -2
Query: 343 TLNFGIRTSFTTLIGVYRWNSGNFTV*IE 257
T F + SFT+ +YRWN N V IE
Sbjct: 140 TFTFSDKPSFTS---IYRWNVTNSNVTIE 165
>SPAC16E8.17c |||succinate-CoA ligase alpha
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 331
Score = 24.6 bits (51), Expect = 9.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 487 ATFTMIIRKAEGIGPKTFARIVDLTKMRTLSVLVGVN 377
A +I+ EGI R+ D+ K ++ S LVG N
Sbjct: 120 AEVPLIVAITEGIPQHDMLRVSDILKTQSKSRLVGPN 156
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 24.6 bits (51), Expect = 9.0
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +2
Query: 83 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 211
Y TP Y P Y P R+P Q+ A + NG PT
Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,303,095
Number of Sequences: 5004
Number of extensions: 50457
Number of successful extensions: 158
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 210309424
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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