Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_E15
(641 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 354 5e-99
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 354 5e-99
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 354 5e-99
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 85 8e-18
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 72 8e-14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 71 2e-13
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 31 0.14
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 4.0
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 26 4.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 26 4.0
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 4.0
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 4.0
SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||... 26 5.3
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 7.0
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 9.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 354 bits (871), Expect = 5e-99
Identities = 156/211 (73%), Positives = 183/211 (86%)
Frame = +2
Query: 8 VAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKALR 187
V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPARPTDK LR
Sbjct: 186 VPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
Query: 188 LPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDN 367
LPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 368 VGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 547
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 548 IACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 640
IACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 354 bits (871), Expect = 5e-99
Identities = 156/211 (73%), Positives = 183/211 (86%)
Frame = +2
Query: 8 VAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKALR 187
V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPARPTDK LR
Sbjct: 186 VPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
Query: 188 LPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDN 367
LPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 368 VGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 547
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 548 IACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 640
IACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 354 bits (871), Expect = 5e-99
Identities = 156/211 (73%), Positives = 183/211 (86%)
Frame = +2
Query: 8 VAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKALR 187
V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPARPTDK LR
Sbjct: 186 VPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLR 245
Query: 188 LPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDN 367
LPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 368 VGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 547
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 548 IACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 640
IACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 85.0 bits (201), Expect = 8e-18
Identities = 57/202 (28%), Positives = 100/202 (49%), Gaps = 3/202 (1%)
Frame = +2
Query: 8 VAFVPISGWHGDNMLE--PSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKA 181
V ++P+S + G N+ + S+ PW+ +G L+E LD++ R +
Sbjct: 421 VKYMPVSAYTGQNVKDRVDSSVCPWY------------QGPSLLEYLDSMTHLERKVNAP 468
Query: 182 LRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSV-EMHHEALQEAVP 358
+P+ YK +GT+ G++E G +K + V+ P N T EV ++ + E + ++
Sbjct: 469 FIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSIC 526
Query: 359 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 538
GD V V+ +++ GYV +KN P F AQ+ +L P ++ GY+ V+ H
Sbjct: 527 GDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIH 584
Query: 539 TAHIACKFAEIKEKVDRRTGKS 604
TA FA++ K+D+ KS
Sbjct: 585 TAVEEVSFAKLLHKLDKTNRKS 606
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 71.7 bits (168), Expect = 8e-14
Identities = 56/177 (31%), Positives = 85/177 (48%), Gaps = 3/177 (1%)
Frame = +2
Query: 8 VAFVPISGWHGDNMLEPSTK--MPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKA 181
V FVPIS G N+++ + W+KG L+ ALD ++PP +P K
Sbjct: 357 VHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPEKPYRKP 404
Query: 182 LRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQE-AVP 358
LRL + DVY+ TV GRVE G ++ V+ + VK+V + + AV
Sbjct: 405 LRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVA 463
Query: 359 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVL 529
GD V + ++ V +LR G + + + NP R F A++ + G I +G T VL
Sbjct: 464 GDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 70.5 bits (165), Expect = 2e-13
Identities = 38/101 (37%), Positives = 60/101 (59%), Gaps = 3/101 (2%)
Frame = +2
Query: 128 LIEALDAILP-PARPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGT--VVVFAPAN 298
L+EA+D+ + P R TD + ++DV+ I G GTV GRVE G LK G +V ++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 299 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 421
+ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 294 LKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 31.1 bits (67), Expect = 0.14
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -3
Query: 405 NSLTDTFLTLKPTLSPGTASCRASWCISTDLTSVVMLAGAKTTTVPGFRIPVSTLPTGTV 226
N+ + + T+ + + ASC S + + +SVV+ + +T TV + VST TGTV
Sbjct: 57 NTTSASVQTIAISQTDNAASCIPSASLLS--SSVVLYSAKETVTVSSYWSLVSTSVTGTV 114
Query: 225 PIPPILYTSC 196
+P +C
Sbjct: 115 YVPYTSSVAC 124
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 4.0
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +1
Query: 448 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 588
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 4.0
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +3
Query: 327 CTTRLYKKLYPVTMLVSTSKTYLSRNCAVVTLQEIRKTTHPGEL 458
CT + KL+PV L T + A+ T+ I T PG++
Sbjct: 196 CTMSIEGKLFPVETLFLQKPTENYVDSAIETVININSTYPPGDI 239
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = -3
Query: 639 SPDLMDLGLSSVDLPVRRSTFS 574
+PDL D+ LSSVD P++ +T S
Sbjct: 30 APDLADVLLSSVDQPLKVNTCS 51
>SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1588
Score = 26.2 bits (55), Expect = 4.0
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = -2
Query: 208 VYVLQGKTESLVSGASRRKDGVEGLNKAFTFSLSFFTLNQPSLEPWHFGGRLQHVI 41
V++ Q + S++ G + + V LNKAF+ S + F + S P HF ++ +V+
Sbjct: 1522 VWLSQAYSPSVLQGTT---ENVAFLNKAFSASANLFDVLPVSNTPSHF-SKMDYVL 1573
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 4.0
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -3
Query: 633 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 514
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 709
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/41 (29%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 324 STDLTSVVMLAGAKTTTVPGFRIPVSTLPTGT--VPIPPIL 208
++DLT+ L +T+ + + + PT T +PIP +L
Sbjct: 615 NSDLTNSSTLLSPTSTSFTSYTVSATATPTSTSHIPIPTVL 655
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 592 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 491
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.0 bits (52), Expect = 9.3
Identities = 15/68 (22%), Positives = 33/68 (48%)
Frame = +3
Query: 357 PVTMLVSTSKTYLSRNCAVVTLQEIRKTTHPGELQTSQRKSLC*ITQVKYQTDTHLYWIA 536
P + +++ S + S + +VT ++ TTH +++T + T + D+H +
Sbjct: 379 PTSSILTNSGSIKSGDHQIVTTSFVQTTTHGSQVETLTYVTTLTETILTTTYDSHTFLTT 438
Query: 537 TQPT*PAN 560
P+ P+N
Sbjct: 439 ITPS-PSN 445
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,932,573
Number of Sequences: 5004
Number of extensions: 63735
Number of successful extensions: 204
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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