BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_B18
(532 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 57 1e-09
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 29 0.43
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 27 1.3
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 26 4.0
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 5.3
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 25 5.3
>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
L22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 57.2 bits (132), Expect = 1e-09
Identities = 27/56 (48%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
Frame = +1
Query: 133 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNYLGNHVVIRRD-KTKVAI 297
+S K+ ID T D I DV FEKYL +R+KV+GKT LG+ VV+ R+ +K+A+
Sbjct: 8 VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAV 63
Score = 29.9 bits (64), Expect = 0.25
Identities = 18/58 (31%), Positives = 25/58 (43%)
Frame = +2
Query: 254 AITLSSAGTRQKSLSTADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDSYELRYF 427
++ +S G+ K A I FS DWLRVV++ YELRY+
Sbjct: 50 SVVVSREGS-SKIAVIAHIDFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRYY 106
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 29.1 bits (62), Expect = 0.43
Identities = 16/66 (24%), Positives = 36/66 (54%), Gaps = 4/66 (6%)
Frame = +1
Query: 196 NFEKYLKERVKV----EGKTNYLGNHVVIRRDKTKVAINRRHSLLKEVSQIPDQALPQEK 363
+F+KYL E + + + K +++ V +RD + R HS+++E+ + A+ K
Sbjct: 684 DFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSERIHSIIEEIELLLSSAIKDSK 743
Query: 364 QSSGLA 381
+++ +A
Sbjct: 744 ETAAIA 749
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 27.5 bits (58), Expect = 1.3
Identities = 19/65 (29%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
Frame = -3
Query: 272 RMTT*LPR*FVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPP 96
R+ T +P+ L +T + F++ R+ G ++++ L ++F +L L RI+ P
Sbjct: 711 RLYTVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGP 767
Query: 95 FTPFL 81
FTPF+
Sbjct: 768 FTPFI 772
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.8 bits (54), Expect = 4.0
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +1
Query: 133 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNYLGNHVVIRRDKTKVA 294
+S K ++ S +VG E++L++ ++ G+T H ++RRD T VA
Sbjct: 135 VSSKHSLGIVEGQSVSHANVG--ERWLEKHLQSVGRTKQELAHELLRRDMTAVA 186
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.4 bits (53), Expect = 5.3
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 366 IFGTGSEWWLLHMTHMSFATSTSMLIATMKTTRIKLF 476
+F S WW L +MS+ + L T+ ++ K F
Sbjct: 1499 VFSHFSSWWNLFSNNMSYPLRSGNLFPTLDSSPNKKF 1535
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 25.4 bits (53), Expect = 5.3
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 5/55 (9%)
Frame = +3
Query: 327 ISNT*PSVTSRKTIFGT-GSEWWLLHMT----HMSFATSTSMLIATMKTTRIKLF 476
I N TS K +FG G WW+L +T H +F T ++ + + +F
Sbjct: 180 ILNNGIKATSTKVLFGLLGLWWWMLFVTASFYHTTFEKCTGFFSGILEWSIVYVF 234
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,953,109
Number of Sequences: 5004
Number of extensions: 36279
Number of successful extensions: 85
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 85
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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