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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0005_B18
         (532 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa...    57   1e-09
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    29   0.43 
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    27   1.3  
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom...    26   4.0  
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    25   5.3  
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch...    25   5.3  

>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
           L22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 57.2 bits (132), Expect = 1e-09
 Identities = 27/56 (48%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
 Frame = +1

Query: 133 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNYLGNHVVIRRD-KTKVAI 297
           +S K+ ID T    D I DV  FEKYL +R+KV+GKT  LG+ VV+ R+  +K+A+
Sbjct: 8   VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAV 63



 Score = 29.9 bits (64), Expect = 0.25
 Identities = 18/58 (31%), Positives = 25/58 (43%)
 Frame = +2

Query: 254 AITLSSAGTRQKSLSTADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDSYELRYF 427
           ++ +S  G+  K    A I FS                  DWLRVV++    YELRY+
Sbjct: 50  SVVVSREGS-SKIAVIAHIDFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRYY 106


>SPAC18G6.05c |||translation elongation regulator Gcn1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 29.1 bits (62), Expect = 0.43
 Identities = 16/66 (24%), Positives = 36/66 (54%), Gaps = 4/66 (6%)
 Frame = +1

Query: 196 NFEKYLKERVKV----EGKTNYLGNHVVIRRDKTKVAINRRHSLLKEVSQIPDQALPQEK 363
           +F+KYL E + +    + K +++    V +RD   +   R HS+++E+  +   A+   K
Sbjct: 684 DFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSERIHSIIEEIELLLSSAIKDSK 743

Query: 364 QSSGLA 381
           +++ +A
Sbjct: 744 ETAAIA 749


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 19/65 (29%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
 Frame = -3

Query: 272 RMTT*LPR*FVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPP 96
           R+ T +P+   L   +T  +  F++    R+    G ++++  L ++F +L  L RI+ P
Sbjct: 711 RLYTVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGP 767

Query: 95  FTPFL 81
           FTPF+
Sbjct: 768 FTPFI 772


>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 25.8 bits (54), Expect = 4.0
 Identities = 16/54 (29%), Positives = 29/54 (53%)
 Frame = +1

Query: 133 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNYLGNHVVIRRDKTKVA 294
           +S K ++        S  +VG  E++L++ ++  G+T     H ++RRD T VA
Sbjct: 135 VSSKHSLGIVEGQSVSHANVG--ERWLEKHLQSVGRTKQELAHELLRRDMTAVA 186


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 2685

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 11/37 (29%), Positives = 18/37 (48%)
 Frame = +3

Query: 366  IFGTGSEWWLLHMTHMSFATSTSMLIATMKTTRIKLF 476
            +F   S WW L   +MS+   +  L  T+ ++  K F
Sbjct: 1499 VFSHFSSWWNLFSNNMSYPLRSGNLFPTLDSSPNKKF 1535


>SPBC543.08 |||phosphoinositide biosynthesis protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 250

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 5/55 (9%)
 Frame = +3

Query: 327 ISNT*PSVTSRKTIFGT-GSEWWLLHMT----HMSFATSTSMLIATMKTTRIKLF 476
           I N     TS K +FG  G  WW+L +T    H +F   T      ++ + + +F
Sbjct: 180 ILNNGIKATSTKVLFGLLGLWWWMLFVTASFYHTTFEKCTGFFSGILEWSIVYVF 234


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,953,109
Number of Sequences: 5004
Number of extensions: 36279
Number of successful extensions: 85
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 85
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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