BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0005_A23
(438 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 27 1.3
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 27 1.7
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 26 2.9
SPAP27G11.09c |||GTP cyclohydrolase |Schizosaccharomyces pombe|c... 26 2.9
SPBC25B2.09c |||arginine-tRNA ligase|Schizosaccharomyces pombe|c... 26 2.9
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 25 5.1
SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 24 8.9
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 24 8.9
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 27.1 bits (57), Expect = 1.3
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -2
Query: 380 IESSI*EQDRRLVEDRGSAERKSVQCSP 297
+ES + QDR+L + S ERKS+ SP
Sbjct: 216 LESKLATQDRKLRMQKKSTERKSLLVSP 243
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 26.6 bits (56), Expect = 1.7
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 249 LTMST*FTMLGTST-LMEQFTLGKTLISPCILGI 151
LT S+ + GT+T L EQF L + ++ PC L I
Sbjct: 449 LTGSSFSHVFGTNTALFEQFVLSRRVMGPCWLKI 482
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.8 bits (54), Expect = 2.9
Identities = 13/38 (34%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = +2
Query: 191 VNCSINV-DVPSIVN*VDIVNIQASDYHTPERNPEEAD 301
++C N D P IV V +N+ D T + NP + D
Sbjct: 62 IHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPAP27G11.09c |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 326
Score = 25.8 bits (54), Expect = 2.9
Identities = 10/42 (23%), Positives = 20/42 (47%)
Frame = +2
Query: 155 PNMQGDISVLPNVNCSINVDVPSIVN*VDIVNIQASDYHTPE 280
PN+ G LP V C + +P++ +++ +D+ E
Sbjct: 54 PNVVGTAERLPKVRCVVRARIPTVKGTEIFLHLYKNDFDNKE 95
>SPBC25B2.09c |||arginine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 618
Score = 25.8 bits (54), Expect = 2.9
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -2
Query: 362 EQDRRLVEDRGSAERKSVQCSPPL 291
E+D ++ +D+ AE K + +PPL
Sbjct: 231 EEDEKIQKDKAEAESKGLPYTPPL 254
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 25.0 bits (52), Expect = 5.1
Identities = 15/52 (28%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Frame = +2
Query: 194 NCSINVDVPSIVN*VDIVNIQASDYHTPERNPEEADYTAP-IYAPQSRDPLL 346
+C I +VP + N+Q + PE N D+ AP Y ++ PL+
Sbjct: 280 SCGIVNEVPQSYDWDSFRNVQRDRWQRPELNYAVVDFIAPQEYMVRAPQPLV 331
>SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit
Rpa2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1227
Score = 24.2 bits (50), Expect = 8.9
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +2
Query: 170 DISVLPNVNCSINVDVPSIV 229
D+S +P++ S+ VD PS+V
Sbjct: 586 DVSQIPSLLLSLGVDPPSVV 605
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 24.2 bits (50), Expect = 8.9
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +2
Query: 149 VIPNMQGDISVLPNVNCSINVDVPSIVN*VDIVNIQASDYHTP-ERNPEE 295
V+PN GD+S+ V + +D+ I+ ++ + P E +PEE
Sbjct: 472 VVPNKDGDLSI---VKIKVRLDLHGILVVEQAYIVEEQEVEEPVETSPEE 518
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,817,733
Number of Sequences: 5004
Number of extensions: 35725
Number of successful extensions: 80
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 158122380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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