BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_M08
(332 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC725.10 |||tspO homolog|Schizosaccharomyces pombe|chr 2|||Manual 45 3e-06
SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces pomb... 28 0.33
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 28 0.43
SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces pom... 26 1.3
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 26 1.3
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 25 2.3
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 25 2.3
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 3.0
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 25 4.0
SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydro... 24 5.3
SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 24 5.3
SPAC5D6.04 |||auxin family transmembrane transporter |Schizosacc... 24 5.3
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 24 5.3
SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein Cwf23|Schiz... 24 7.0
SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces p... 24 7.0
SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharo... 24 7.0
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 23 9.2
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 23 9.2
SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 23 9.2
>SPBC725.10 |||tspO homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 162
Score = 44.8 bits (101), Expect = 3e-06
Identities = 26/73 (35%), Positives = 38/73 (52%)
Frame = +1
Query: 34 VMANWQAIGSIILPNVGGWVNGLYFAGQIRKGEEKSWYDSLKKPSWTPPKWVFGPAWTGL 213
+ NW + ++P GW F G K + Y++ K+P + PP FGPAWT L
Sbjct: 11 ISKNWWSAS--LVPVACGW-----FIGNSYKPRKD--YENKKQPKFHPPASAFGPAWTLL 61
Query: 214 YSGMGIASYLVWE 252
Y MG AS+L ++
Sbjct: 62 YLTMGYASHLAYK 74
>SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 561
Score = 28.3 bits (60), Expect = 0.33
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +2
Query: 236 PIWCGRNVADLPDEAVLPLSLYGGQTAPEL 325
P++ G A LP+ + +++YG + AP L
Sbjct: 71 PVYFGVGTAGLPESIISHMAMYGPEVAPNL 100
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 27.9 bits (59), Expect = 0.43
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = -1
Query: 107 KYSPLTQPPTFGRIIEPMACQFAITKSCV 21
KY T PP+ + P+A ++ I SC+
Sbjct: 370 KYFDFTNPPSRSCVAAPLASEYRIVMSCI 398
>SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 184
Score = 26.2 bits (55), Expect = 1.3
Identities = 16/45 (35%), Positives = 28/45 (62%), Gaps = 4/45 (8%)
Frame = +3
Query: 201 LDGSLQWNGYRLL--SGVGGMW--RIYLMRQFYLLAYTVGKLLLN 323
+D ++ + YR L SG+ ++ RI L+R +Y++ YT+ LLN
Sbjct: 26 VDRTIPYTTYRWLTVSGLIALFFIRILLVRGWYIVCYTLAIYLLN 70
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 26.2 bits (55), Expect = 1.3
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = -3
Query: 147 VPR---FLFTLPDLARKVQPVNPTTNVW*DYRTNGLPVR 40
VPR F LP++ R + P P + W DY NG+P++
Sbjct: 36 VPRQSYFAQILPNVQRLLAPSIPLSECWLDY--NGVPLK 72
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 25.4 bits (53), Expect = 2.3
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +2
Query: 233 PPIWCGRNVADLPDEAVLPLSLYGGQ 310
PP+ CG + D P VLP S + Q
Sbjct: 631 PPVACGTPIPDCPYLCVLPKSCHHPQ 656
>SPAC23C4.19 |spt5||transcription elongation factor
Spt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 990
Score = 25.4 bits (53), Expect = 2.3
Identities = 14/51 (27%), Positives = 17/51 (33%), Gaps = 7/51 (13%)
Frame = +1
Query: 73 PNVGGWVNGLYFAGQIRKGEEKSWYDSLKKPSWT----PPKWVFG---PAW 204
PNV W G +W + P+W P W G PAW
Sbjct: 807 PNVPNWAQGARTPAVANGSRTPAWNTGSRTPAWNSGSKTPAWNSGSRTPAW 857
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 25.0 bits (52), Expect = 3.0
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = +1
Query: 127 GEEKSWYDSLKKPSWTPPKWVFGP 198
GEE + + KP PP W GP
Sbjct: 521 GEEHQGAEQIDKPFSCPPTWNTGP 544
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 24.6 bits (51), Expect = 4.0
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +3
Query: 9 FLVTNTRFRYGELASHWFYNPTK 77
F V +RF+ L SH+ Y P K
Sbjct: 1358 FTVLQSRFQRDHLWSHYLYQPLK 1380
>SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydroxy-
6-
hydroxymethyldihydropteridinediphosphokinase/dihydroneop
terinaldolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 24.2 bits (50), Expect = 5.3
Identities = 10/38 (26%), Positives = 20/38 (52%), Gaps = 3/38 (7%)
Frame = +1
Query: 145 YDSLKKPSWTPPKWVFGPA---WTGLYSGMGIASYLVW 249
++ +KKP + W+ GP+ +TG ++G +W
Sbjct: 610 HELMKKPQFKDMHWLSGPSRKGFTGYFTGDASPKDRIW 647
>SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 457
Score = 24.2 bits (50), Expect = 5.3
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = +2
Query: 89 GLTGCTLRARSGRVKRNLGTIH*RNPAGHLLNGCLDQPGRVFTVE 223
G+TGC L A GR R + + + G L QPG + +E
Sbjct: 14 GITGCALGAALGRQGRKVLVLERDMSEPDRIVGELLQPGGIEALE 58
>SPAC5D6.04 |||auxin family transmembrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 452
Score = 24.2 bits (50), Expect = 5.3
Identities = 12/43 (27%), Positives = 25/43 (58%)
Frame = +2
Query: 11 FSNKHKISLWRTGKPLVL*SYQTLVVGLTGCTLRARSGRVKRN 139
FS+ +I++W +P++ + +V+ L G L A+ G + R+
Sbjct: 4 FSSLGQINVWSLLRPIIESDLEVIVIALGGYVL-AKKGFLPRD 45
>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 588
Score = 24.2 bits (50), Expect = 5.3
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = +3
Query: 228 YRLLSGVGGMWRIYLMRQFYLLAYTVG 308
YR +SG G IY M L+ + VG
Sbjct: 506 YRYISGFGAALNIYFMIICNLIGFAVG 532
>SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein
Cwf23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 289
Score = 23.8 bits (49), Expect = 7.0
Identities = 5/15 (33%), Positives = 12/15 (80%)
Frame = +2
Query: 194 DQPGRVFTVEWVSPP 238
++P +++ V+W+ PP
Sbjct: 229 EKPSKIYDVQWLKPP 243
>SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 472
Score = 23.8 bits (49), Expect = 7.0
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 130 EEKSWYDSLKKPSWTPPK 183
+EK S+ +PSW PPK
Sbjct: 126 KEKQAVLSIGRPSWLPPK 143
>SPCC1020.03 |||mitochondrial iron ion
transporter|Schizosaccharomyces pombe|chr 3|||Manual
Length = 397
Score = 23.8 bits (49), Expect = 7.0
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +1
Query: 199 AWTGLYSGMGIAS 237
AW GLYS +G+A+
Sbjct: 77 AWLGLYSNIGLAA 89
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 23.4 bits (48), Expect = 9.2
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = +3
Query: 45 LASHWFYNPTKRWWL 89
L H +YNP K WL
Sbjct: 631 LLRHTYYNPAKEAWL 645
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 23.4 bits (48), Expect = 9.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 22 TQDFVMANWQAIGSIILPNVGGWVNGLYFAGQIRKGEEKS 141
T+D + + G I+L N G G + ++KGE+ S
Sbjct: 424 TRDGQLDTMRKAGGIVLANACGPCIGQWKRTDVKKGEKNS 463
>SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 388
Score = 23.4 bits (48), Expect = 9.2
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -2
Query: 220 HCKDPSRLVQTPI*EVSSWVSSMNRTKISLHP 125
H PS+L Q + E++ WV M ++ L P
Sbjct: 159 HLHQPSKLTQNSLREMTVWV--METMRLFLRP 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,750,506
Number of Sequences: 5004
Number of extensions: 39436
Number of successful extensions: 104
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 93942212
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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