BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_L14
(614 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.14c |btf3|egd1, btt1, nac2|nascent polypeptide-associat... 103 2e-23
SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 28 0.93
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 5.0
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 6.6
>SPAC4F10.14c |btf3|egd1, btt1, nac2|nascent polypeptide-associated
complex beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 151
Score = 103 bits (248), Expect = 2e-23
Identities = 60/136 (44%), Positives = 78/136 (57%), Gaps = 8/136 (5%)
Frame = +3
Query: 3 RIGGKGTPRRKKKVVHVTA--ATDDXXXXXXXXXXXVNTIPGIEEVNMIKDDGTVIHFNN 176
RIGGKGTPRRK K +A A DD + + GI+EVNM K+DG VI+F
Sbjct: 14 RIGGKGTPRRKVKKPSKSAMSAADDKKVQGALKKLNMQNLAGIQEVNMFKEDGGVINFRA 73
Query: 177 PKAQASLAANTFAITGHGENKQIAEMLPGILSQLGPEGLNQLKRLASSV---AAPKPLD- 344
P +SL T AI G E K ++E+LPGIL+ LGPE L L+++A + K D
Sbjct: 74 PTVHSSLPNETTAIYGKAEEKTLSEILPGILNNLGPESLTALRQMAEQLKVSEGEKGADA 133
Query: 345 --EDDEVPNLVGNFDE 386
+D E+P+LV FDE
Sbjct: 134 QADDGEIPDLVEKFDE 149
>SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 658
Score = 28.3 bits (60), Expect = 0.93
Identities = 15/38 (39%), Positives = 19/38 (50%)
Frame = +2
Query: 29 AQEEGCTRYSGNRR*EIAIVSQKIVSEHHSWH*RGQYD 142
A EE T GN + S+K S +HS H +G YD
Sbjct: 391 AWEEDLTPQYGNLTTRLLFASKKYWSRNHSSHSQGNYD 428
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.8 bits (54), Expect = 5.0
Identities = 19/86 (22%), Positives = 38/86 (44%), Gaps = 3/86 (3%)
Frame = +3
Query: 108 NTIPGIEEVNMIKDDGTVIH---FNNPKAQASLAANTFAITGHGENKQIAEMLPGILSQL 278
++IP ++++ IK+ V H N K Q ++A+ + G K+ A +
Sbjct: 326 SSIPNVDDIRSIKNSWKVFHDEKLNELKKQVEISASAAQLNGLYPQKKRA-FVSHFSQNR 384
Query: 279 GPEGLNQLKRLASSVAAPKPLDEDDE 356
P + + + SS + +P + DE
Sbjct: 385 KPYSQSDISKAQSSSFSEEPSNIYDE 410
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.4 bits (53), Expect = 6.6
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -2
Query: 169 KCITVPSSFIILTSSMPGMVFTDNFLRDDCNF 74
+C+ + SFI+L S + T+NFL F
Sbjct: 1238 ECLPIKESFIVLLGSCIKQLKTENFLEYKVTF 1269
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,243,511
Number of Sequences: 5004
Number of extensions: 39101
Number of successful extensions: 99
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 97
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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