BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_K15
(512 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 64 2e-11
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 28 0.94
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 27 2.2
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 26 2.9
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 5.0
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 8.8
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 25 8.8
>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
L22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 63.7 bits (148), Expect = 2e-11
Identities = 30/59 (50%), Positives = 42/59 (71%), Gaps = 1/59 (1%)
Frame = +3
Query: 126 KISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARD-KTKVAINA 299
K+S K+ ID T D I DV FEKYL +R+KV+GKT NLG+ VV++R+ +K+A+ A
Sbjct: 7 KVSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIA 65
Score = 29.1 bits (62), Expect = 0.41
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 370 DWLRVVASAHDSYELRYF 423
DWLRVV++ YELRY+
Sbjct: 89 DWLRVVSTKKGVYELRYY 106
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 27.9 bits (59), Expect = 0.94
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = -2
Query: 187 TSRMLSSAGCVQSMVNLRLIFLLAPLPRFCHLLLLSYQSSGV 62
+S LS C+ S++NL +I + + HLL Y++S +
Sbjct: 288 SSLQLSEEACISSLLNLGMIKVFSLAGNCLHLLHTEYKNSSL 329
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 26.6 bits (56), Expect = 2.2
Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +3
Query: 210 KERVKVEGKTNNLGNHVVIARDKTKV-AINAEHSLLKEVSQILDQALPQEKQ 362
K+ K++ K N G+ + K KV A+N + K+ ++LD+ + QEKQ
Sbjct: 527 KDMAKLQNKLNMTGSEQQ-KKAKAKVKAMNKKLEKDKQSGKVLDEEIIQEKQ 577
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 26.2 bits (55), Expect = 2.9
Identities = 16/59 (27%), Positives = 28/59 (47%), Gaps = 5/59 (8%)
Frame = +2
Query: 323 ISNT*PSVTSRKTIFGT-GSEWWLLHMT----HMSFATSTSMLIATMKTTRIKLFINKI 484
I N TS K +FG G WW+L +T H +F T ++ + + +F +++
Sbjct: 180 ILNNGIKATSTKVLFGLLGLWWWMLFVTASFYHTTFEKCTGFFSGILEWSIVYVFSSRM 238
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.4 bits (53), Expect = 5.0
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +2
Query: 362 IFGTGSEWWLLHMTHMSFATSTSMLIATMKTTRIKLF 472
+F S WW L +MS+ + L T+ ++ K F
Sbjct: 1499 VFSHFSSWWNLFSNNMSYPLRSGNLFPTLDSSPNKKF 1535
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 24.6 bits (51), Expect = 8.8
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +3
Query: 183 DVGNFEKYLKERVKVEGKTNNLGNH 257
+ GN +KY + +KV GK ++ H
Sbjct: 955 EAGNLKKYDQPNLKVSGKNDSFVTH 979
>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 968
Score = 24.6 bits (51), Expect = 8.8
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = +3
Query: 120 KRKISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNL 248
K + K ++D H + L N + +KE+ KV+G+ + L
Sbjct: 147 KEDLHKKLSVDNAHLIKQLDLLSSNMKTLMKEKTKVQGQRDLL 189
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,917,294
Number of Sequences: 5004
Number of extensions: 36213
Number of successful extensions: 94
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 94
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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