BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_H24
(238 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P39656 Cluster: Dolichyl-diphosphooligosaccharide--prot... 62 2e-09
UniRef50_Q9LVR2 Cluster: Dolichyl-di-phosphooligosaccharide-prot... 51 6e-06
UniRef50_Q54E62 Cluster: Dolichyl-diphosphooligosaccharide-prote... 46 2e-04
UniRef50_Q23AP5 Cluster: Dolichyl-diphosphooligosaccharide-prote... 39 0.019
UniRef50_Q4P4G9 Cluster: Putative uncharacterized protein; n=1; ... 38 0.043
UniRef50_Q5KAG9 Cluster: Dolichyl-diphosphooligosaccharide--prot... 37 0.075
UniRef50_Q03HK9 Cluster: Predicted esterase of the alpha-beta hy... 32 2.8
>UniRef50_P39656 Cluster: Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor; n=73;
Eumetazoa|Rep:
Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor - Homo
sapiens (Human)
Length = 439
Score = 62.1 bits (144), Expect = 2e-09
Identities = 26/36 (72%), Positives = 33/36 (91%)
Frame = +3
Query: 39 KTLVLVDNLNIRETHSQFFKSLQERGYSLTFKLADD 146
+TLVL+DNLN+RETHS FF+SL++RG+ LTFK ADD
Sbjct: 29 RTLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADD 64
Score = 31.5 bits (68), Expect = 3.8
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +2
Query: 164 LNMGNISYKNLIIFSPSVVECGGQI 238
+ G Y NLIIFSPSV + GG I
Sbjct: 70 IKYGEFLYDNLIIFSPSVEDFGGNI 94
>UniRef50_Q9LVR2 Cluster: Dolichyl-di-phosphooligosaccharide-protein
glycotransferase (Oligosaccharyltransferase)-like; n=10;
Magnoliophyta|Rep:
Dolichyl-di-phosphooligosaccharide-protein
glycotransferase (Oligosaccharyltransferase)-like -
Arabidopsis thaliana (Mouse-ear cress)
Length = 442
Score = 50.8 bits (116), Expect = 6e-06
Identities = 20/40 (50%), Positives = 33/40 (82%)
Frame = +3
Query: 33 DQKTLVLVDNLNIRETHSQFFKSLQERGYSLTFKLADDAK 152
D++ LVL+D+L+++ +HS FF +L+ RG+ L FKLA+D+K
Sbjct: 30 DRRVLVLLDDLSLKSSHSIFFNTLKSRGFDLDFKLAEDSK 69
>UniRef50_Q54E62 Cluster: Dolichyl-diphosphooligosaccharide-protein
glycotransferase; n=1; Dictyostelium discoideum AX4|Rep:
Dolichyl-diphosphooligosaccharide-protein
glycotransferase - Dictyostelium discoideum AX4
Length = 426
Score = 45.6 bits (103), Expect = 2e-04
Identities = 17/36 (47%), Positives = 30/36 (83%)
Frame = +3
Query: 27 LADQKTLVLVDNLNIRETHSQFFKSLQERGYSLTFK 134
+ ++TLV++D+L+I++THS FFK+L+ +GY L F+
Sbjct: 24 IGGKRTLVVLDDLSIKKTHSTFFKNLENKGYKLQFE 59
>UniRef50_Q23AP5 Cluster: Dolichyl-diphosphooligosaccharide-protein
glycosyltransferase 48kD subunit containing protein;
n=1; Tetrahymena thermophila SB210|Rep:
Dolichyl-diphosphooligosaccharide-protein
glycosyltransferase 48kD subunit containing protein -
Tetrahymena thermophila SB210
Length = 430
Score = 39.1 bits (87), Expect = 0.019
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +3
Query: 24 SLADQKTLVLVDNLNIRETHSQFFKSLQERGYSLTFKLADDA 149
SL + +V+VD+ + + THS FF L+ +GY L F+ + A
Sbjct: 26 SLLKNRVIVVVDSSSYQHTHSTFFNDLRSKGYDLDFRALNSA 67
>UniRef50_Q4P4G9 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 576
Score = 37.9 bits (84), Expect = 0.043
Identities = 20/51 (39%), Positives = 26/51 (50%)
Frame = +3
Query: 12 CLSLSLADQKTLVLVDNLNIRETHSQFFKSLQERGYSLTFKLADDAKPGAV 164
C S + LV+VD + + T+S F SL RGY + FK D KP V
Sbjct: 33 CALPSATGDRILVVVDQ-SAKSTYSNLFASLSSRGYKVDFKAPRDTKPELV 82
>UniRef50_Q5KAG9 Cluster: Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit, putative; n=1;
Filobasidiella neoformans|Rep:
Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit, putative -
Cryptococcus neoformans (Filobasidiella neoformans)
Length = 468
Score = 37.1 bits (82), Expect = 0.075
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3
Query: 24 SLADQKTLVLVDNLNIRETHSQFFKSLQERGYSLTFK 134
S + LV++++ ++ +SQF+ SLQ+RG+ LTFK
Sbjct: 22 SATGDRVLVVLESAVTKDDYSQFWNSLQQRGFELTFK 58
>UniRef50_Q03HK9 Cluster: Predicted esterase of the alpha-beta
hydrolase superfamily; n=1; Pediococcus pentosaceus ATCC
25745|Rep: Predicted esterase of the alpha-beta
hydrolase superfamily - Pediococcus pentosaceus (strain
ATCC 25745 / 183-1w)
Length = 284
Score = 31.9 bits (69), Expect = 2.8
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = -2
Query: 228 PHSTTDGENMIKFLYEIFPIFRQHQVSHHRQA*TSSYNRALAKI*RIENESPLYL 64
P ++ ++KF Y FP F Q +Q YNR+LA+I R+E E +++
Sbjct: 192 PENSRAMNRLVKFRYRDFPRFVQAFADRPQQ-----YNRSLAEIERLEREGKIFV 241
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 223,701,072
Number of Sequences: 1657284
Number of extensions: 3347569
Number of successful extensions: 7202
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7201
length of database: 575,637,011
effective HSP length: 57
effective length of database: 481,171,823
effective search space used: 10104608283
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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