Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_H08
(499 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 113 1e-26
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 73 2e-14
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 63 3e-11
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 45 7e-06
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 41 9e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 41 9e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 39 5e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.004
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 30 0.17
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 0.68
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 27 1.6
SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 2.7
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce... 26 3.6
SPAC1039.04 |||nicotinic acid plasma membrane transporter |Schiz... 25 4.8
SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase I|Schizosacchar... 25 6.3
SPAC10F6.08c |||HMG box protein|Schizosaccharomyces pombe|chr 1|... 25 8.4
SPAC644.04 |pct1||RNA 5'-triphosphatase|Schizosaccharomyces pomb... 25 8.4
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 8.4
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 113 bits (273), Expect = 1e-26
Identities = 53/123 (43%), Positives = 78/123 (63%)
Frame = +3
Query: 90 YLCSAAEEDVLDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPP 269
+ C++AE V + + ++ +V FYAPWCGHCK L PEY AA L+KD
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDG-- 72
Query: 270 VSLAKVDCTEGGKSTCEKFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKYMRAQVGPSS 449
+SL +VDCTE G C ++S+ GYPTL +F+ G+ S Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 450 RDL 458
+ +
Sbjct: 132 KPI 134
Score = 69.7 bits (163), Expect = 2e-13
Identities = 37/90 (41%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +3
Query: 105 AEEDVLDLTDSDFSS-VLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLA 281
++ED++ L +F V+D+ LV FYAPWCGHCK L P Y A DD V +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY-SDDSNVVVA 411
Query: 282 KVDCTEGGKSTCEKFSVSGYPTLKIFRKGE 371
K+D TE S S+SG+PT+ F+ +
Sbjct: 412 KIDATENDISV----SISGFPTIMFFKAND 437
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 73.3 bits (172), Expect = 2e-14
Identities = 41/123 (33%), Positives = 65/123 (52%), Gaps = 6/123 (4%)
Frame = +3
Query: 114 DVLDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDC 293
+ ++L +F + +LV+FYAPWCG+CK+L P Y A L P + VDC
Sbjct: 32 NTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSLLP---VTAVDC 88
Query: 294 -TEGGKSTCEKFSVSGYPTLKIF---RKGE--LSSDYNGPRESNGIVKYMRAQVGPSSRD 455
+ ++ C ++ V G+PT+K+ KG S+DYNG R + K++ + PS
Sbjct: 89 DADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PSKVK 147
Query: 456 LLT 464
+LT
Sbjct: 148 ILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 62.9 bits (146), Expect = 3e-11
Identities = 35/112 (31%), Positives = 57/112 (50%), Gaps = 3/112 (2%)
Frame = +3
Query: 114 DVLDLTDSDFSSV-LDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVD 290
+V++L +F V +D LV FYA WCG+CKRL P Y G + K++P V + K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYE-TLGKVFKNEPNVEIVKIN 199
Query: 291 CTEGGKSTCEKFSVSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVG 440
+ V+ +PT+K F K + Y G R +++Y+ + G
Sbjct: 200 -ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
Score = 60.9 bits (141), Expect = 1e-10
Identities = 38/139 (27%), Positives = 65/139 (46%), Gaps = 1/139 (0%)
Frame = +3
Query: 48 MSVSLKSLFLLGLVYLCSAAEEDVLDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPE 227
M + L S + L L A+ L + +++ AL+ FYA WCGHCK L P
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPV 60
Query: 228 YAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLKIF-RKGELSSDYNGPRES 404
Y G L +D V + K+D + +K+ ++G+PTL F G Y+ R+
Sbjct: 61 YE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDV 118
Query: 405 NGIVKYMRAQVGPSSRDLL 461
+ + +++ + G R ++
Sbjct: 119 DSLTQFVSEKTGIKKRKIV 137
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 44.8 bits (101), Expect = 7e-06
Identities = 28/94 (29%), Positives = 46/94 (48%)
Frame = +3
Query: 141 FSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCE 320
F + + A VMF + HC+ AV + I + D + +A+V+C E K C
Sbjct: 189 FGNEIMSKTRAFVMFVS--LKHCEDCFHWEAVWSSITRNTDERLKMAQVNCDE-EKEMCN 245
Query: 321 KFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKY 422
F + +PT ++F +G S YNGP + ++ Y
Sbjct: 246 HFHIKKFPTFRVF-QGFDSIQYNGPLKYQQLLSY 278
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 41.1 bits (92), Expect = 9e-05
Identities = 27/112 (24%), Positives = 53/112 (47%), Gaps = 4/112 (3%)
Frame = +3
Query: 126 LTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKP--EYAVAAGILKKDDPPVSLAKVDCTE 299
LTD+D S + + T + +Y P CG CKRL P + V + + +VDC++
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 300 GGKSTCEKFSVSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVGPSS 449
S+C ++ PTL +++ GE+ + + ++ ++ + P +
Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDT 138
Score = 35.9 bits (79), Expect = 0.003
Identities = 21/97 (21%), Positives = 44/97 (45%)
Frame = +3
Query: 132 DSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKS 311
D+D + L + + FY+ C C + + A ++ +++A ++C K
Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGK---LNVAHINCAVS-KR 343
Query: 312 TCEKFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKY 422
C+++S+ +PT +F K E +Y G +V +
Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 41.1 bits (92), Expect = 9e-05
Identities = 24/78 (30%), Positives = 37/78 (47%)
Frame = +3
Query: 138 DFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTC 317
D+++ + +V FYA WCG CK LKP L + + S V+ +
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKPFLEK----LSEQNQKASFIAVN-ADKFSDIA 81
Query: 318 EKFSVSGYPTLKIFRKGE 371
+K V PT+ +FRKG+
Sbjct: 82 QKNGVYALPTMVLFRKGQ 99
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 38.7 bits (86), Expect = 5e-04
Identities = 21/79 (26%), Positives = 37/79 (46%)
Frame = +3
Query: 135 SDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKST 314
S+F S++ Q +V F+A WCG CK + P++ + + KVD + +
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYS----DATFIKVDVDQLSEIA 64
Query: 315 CEKFSVSGYPTLKIFRKGE 371
E V P+ +++ GE
Sbjct: 65 AEA-GVHAMPSFFLYKNGE 82
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 35.5 bits (78), Expect = 0.004
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +3
Query: 186 YAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLKIFRK 365
YA WCG CK + P ++ A K P AKV+ E + V PT F
Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83
Query: 366 GE 371
G+
Sbjct: 84 GK 85
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 30.3 bits (65), Expect = 0.17
Identities = 17/49 (34%), Positives = 30/49 (61%), Gaps = 2/49 (4%)
Frame = -1
Query: 154 NTDEKSESVRSNTSSSA-AEHKYTNPSKNKDFSDTDI-IKSRRQLL*NR 14
N K E ++ SA +HK PS+N++ +D +I I++RRQ++ N+
Sbjct: 399 NICSKEELYETDLKESAPTQHKQPAPSENENKADQEIDIEARRQIIKNK 447
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 28.3 bits (60), Expect = 0.68
Identities = 18/73 (24%), Positives = 30/73 (41%)
Frame = +3
Query: 174 LVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLK 353
L+ FYAPW CK++ + A KD K++ E E F V+ P
Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78
Query: 354 IFRKGELSSDYNG 392
+ ++ + +G
Sbjct: 79 LIHGAKVLARISG 91
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.1 bits (57), Expect = 1.6
Identities = 18/53 (33%), Positives = 31/53 (58%)
Frame = -1
Query: 181 MTKAVSC*SNTDEKSESVRSNTSSSAAEHKYTNPSKNKDFSDTDIIKSRRQLL 23
++KA+ +T + S+ +NTSS+A ++K P+K+ FSD SR + L
Sbjct: 243 VSKAIQLVKSTSDLG-SLSTNTSSTAQKNKSRKPTKS--FSDAVAAASRAKEL 292
>SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 279
Score = 26.2 bits (55), Expect = 2.7
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +3
Query: 306 KSTCEKFSVSGYPTLKIFRKGELSSDYNGPRESN 407
KS EK + Y LK+ + E + DYN P N
Sbjct: 37 KSPLEKEIANEYEALKVTERKEDTQDYNEPELHN 70
>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 642
Score = 25.8 bits (54), Expect = 3.6
Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = -1
Query: 157 SNTDEKSESVRSNTSSSAAEHKYTNPSKN--KDFSDTDI 47
+ DE+S S +SN+ S A+ K + K F+DT +
Sbjct: 93 AENDEESSSQKSNSKESHAQRKKLQKERKAMKPFADTSL 131
>SPAC1039.04 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 25.4 bits (53), Expect = 4.8
Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 5/63 (7%)
Frame = +3
Query: 24 NNCRRDLIMSVSLKSLFLLGLVYLCSAAEEDVLDLT-----DSDFSSVLDQHDTALVMFY 188
+ R L + L LL L+YL +A ++ + D D V DQ++ + +FY
Sbjct: 47 HKAERRLCRKFDFRILPLLALLYLFNALDKSNVSNAKTNGMDKDLGFVGDQYNIMISIFY 106
Query: 189 APW 197
P+
Sbjct: 107 IPF 109
>SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase
I|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 25.0 bits (52), Expect = 6.3
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 18 FYNNCRRDLIMSVSLKSLFLLGLV 89
FY N R ++++ L +LFL+G+V
Sbjct: 786 FYKNFRTYVVLTWILSNLFLVGIV 809
>SPAC10F6.08c |||HMG box protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 24.6 bits (51), Expect = 8.4
Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 2/37 (5%)
Frame = -1
Query: 154 NTDEKSESVRSNTSSSAAEHKYTNPSKNKDFS--DTD 50
+T+E S++ + ++S HK +N S+ KD S DTD
Sbjct: 110 STEETSDTNVALNNTSEISHKSSNNSQPKDASVNDTD 146
>SPAC644.04 |pct1||RNA 5'-triphosphatase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -1
Query: 127 RSNTSSSAAEHKYTNPSKNKDFSDT 53
R + +A+EHKY N N+ F D+
Sbjct: 114 RFESDMTASEHKYLNEFLNQAFRDS 138
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 340 YPDTENFSHVLLPPSVQSTFAN 275
YP+ N H LL S+ S +AN
Sbjct: 436 YPELSNLYHKLLHISISSIYAN 457
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,953,222
Number of Sequences: 5004
Number of extensions: 38348
Number of successful extensions: 131
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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