Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0004_G17
(546 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 317 6e-88
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 310 9e-86
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 147 1e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 92 4e-20
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 29 0.34
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 0.78
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 3.2
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 3.2
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 4.2
SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 25 5.5
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 5.5
SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces pom... 25 9.6
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 25 9.6
SPBC29A10.16c |||cytochrome b5 |Schizosaccharomyces pombe|chr 2|... 25 9.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 317 bits (779), Expect = 6e-88
Identities = 140/181 (77%), Positives = 159/181 (87%)
Frame = +3
Query: 3 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 182
LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+L
Sbjct: 193 LTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252
Query: 183 NVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 362
NVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR
Sbjct: 253 NVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 312
Query: 363 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 542
G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +
Sbjct: 313 TGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGI 372
Query: 543 A 545
A
Sbjct: 373 A 373
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 310 bits (761), Expect = 9e-86
Identities = 137/181 (75%), Positives = 157/181 (86%)
Frame = +3
Query: 3 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 182
LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+L
Sbjct: 189 LTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSL 248
Query: 183 NVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 362
NVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR
Sbjct: 249 NVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 308
Query: 363 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 542
G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 543 A 545
A
Sbjct: 369 A 369
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 147 bits (356), Expect = 1e-36
Identities = 66/172 (38%), Positives = 103/172 (59%)
Frame = +3
Query: 3 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGAL 182
L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G L
Sbjct: 187 LSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGEL 246
Query: 183 NVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 362
N DL + N+VP+PR+HF +V +AP+ + + + +SV E+T F+ N MV DPR
Sbjct: 247 NSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPR 306
Query: 363 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 518
HG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 307 HGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 92.3 bits (219), Expect = 4e-20
Identities = 46/170 (27%), Positives = 88/170 (51%), Gaps = 4/170 (2%)
Frame = +3
Query: 27 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQ 206
++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSII 257
Query: 207 TNLVPYPRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKY 374
+L+P PR HF L +Y P + E + +V ++ P NQMV +P + +
Sbjct: 258 ASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCF 317
Query: 375 MACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTV 524
++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P +
Sbjct: 318 ISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYI 367
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 29.5 bits (63), Expect = 0.34
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +1
Query: 34 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 147
++ S+ T KP + TW +N R P S+ + H
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 28.3 bits (60), Expect = 0.78
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = +3
Query: 309 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 434
+ ACFEP N C H K C L + KD N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 135
Score = 26.2 bits (55), Expect = 3.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2
Query: 188 DIEGAVETQRGGNR*YDLADKTIEVGVGRAFN 93
DIE E GN+ ++L +K I++ G+ FN
Sbjct: 58 DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 26.2 bits (55), Expect = 3.2
Identities = 16/67 (23%), Positives = 26/67 (38%)
Frame = +1
Query: 19 PWNTPIVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*R 198
P+ W T KP + A W ++ + + + S +PP S A +
Sbjct: 419 PYKHRYPYDWRTHKPLILRATPQWFISLENERKTAIKALDSVKMIPP--NSRARLLGF-L 475
Query: 199 SSRPTWC 219
+ RP WC
Sbjct: 476 NGRPEWC 482
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.8 bits (54), Expect = 4.2
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +1
Query: 91 ILNARPTPTSIVLSARSYHRL 153
I NAR T V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519
>SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1066
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = -2
Query: 440 GSDSRVDVLGHDVTAVQHAAGHVFAVT 360
G+D + GH V +Q A G VF+ T
Sbjct: 421 GADEKPKYAGHVVAVLQRAPGQVFSGT 447
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.4 bits (53), Expect = 5.5
Identities = 19/67 (28%), Positives = 30/67 (44%)
Frame = +1
Query: 16 PPWNTPIVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI* 195
PPW ST S TS+ W A+P ++ + + + P +AP++S+
Sbjct: 407 PPWAAA-----STSVSSSTSSPAPW---AKPASSAAPSNPAPWQQ--PAAPQSAPALSMN 456
Query: 196 RSSRPTW 216
SS P W
Sbjct: 457 PSSLPPW 463
>SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 409
Score = 24.6 bits (51), Expect = 9.6
Identities = 26/113 (23%), Positives = 45/113 (39%), Gaps = 1/113 (0%)
Frame = +3
Query: 21 LEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTE 200
L SD +NE YDI +N + E N I + + + + + L++ E
Sbjct: 61 LSESDVENAKNNEHTYDIDNKNFNFEPKNSVNKTETIREELDQAASKILIE-TLDLHQKE 119
Query: 201 -FQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCD 356
F+ +V T A + A+ H + + E+ E AN++V D
Sbjct: 120 GFEETIVTEE------ATDAVDLEAKGTTHGENDLDEVPTELNELANEVVDVD 166
>SPBC428.07 |meu6||meiotic chromosome segregation protein
Meu6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 651
Score = 24.6 bits (51), Expect = 9.6
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 177 ALNVDLTEFQTNLVPYPRIHFPLVT 251
A +V++ E +TN +P HFP T
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATT 392
>SPBC29A10.16c |||cytochrome b5 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 124
Score = 24.6 bits (51), Expect = 9.6
Identities = 14/50 (28%), Positives = 20/50 (40%)
Frame = -2
Query: 440 GSDSRVDVLGHDVTAVQHAAGHVFAVTRVTFHHLVGGLEASVRDLRYREL 291
G D +D G D T GH A + +G L+ + R +EL
Sbjct: 41 GLDIMLDYAGQDATKAYQDIGHSIAADELLEEMYIGDLKPGTEE-RLKEL 89
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,375,960
Number of Sequences: 5004
Number of extensions: 47783
Number of successful extensions: 162
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 225926624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -