Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_P09
(356 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 97 5e-22
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 65 3e-12
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 61 4e-11
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 61 4e-11
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 57 9e-10
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 50 1e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 37 0.001
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 36 0.001
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 36 0.001
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 36 0.001
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 36 0.001
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 31 0.040
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 31 0.070
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 28 0.37
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 2.6
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 25 3.5
SPCC1442.15c |cox18||mitochondrial inner membrane protein Cox18|... 25 4.6
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 24 6.1
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 24 6.1
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 24 6.1
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 24 8.0
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 97.5 bits (232), Expect = 5e-22
Identities = 51/104 (49%), Positives = 70/104 (67%), Gaps = 6/104 (5%)
Frame = +3
Query: 63 FQSKYLFRRMCSSE--PSSKT----INVKKIRNFSIVAHVDHGKSTLADRLLELTGVIKP 224
F Y R++ S + PS + I ++RN++++AH+DHGKSTL+D +L+LTGVI
Sbjct: 28 FSVSYASRKLASEDNKPSIQEVVRGIPQNRVRNWAVIAHIDHGKSTLSDCILKLTGVINE 87
Query: 225 GADHAQVLDQLQVERERGITVKAVTASLNYSYNNEKYLLNLIDT 356
Q LD+L+VER RGITVKA T S+ Y Y+ + YLLNLIDT
Sbjct: 88 HNFRNQFLDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDT 131
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 65.3 bits (152), Expect = 3e-12
Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 9/87 (10%)
Frame = +3
Query: 123 NVKKIRNFSIVAHVDHGKSTLADRLLELTGVIKPG-ADHAQVLDQLQVERERGITVKAVT 299
N + IRNF+++AHVDHGK+TLAD LL G+I A + LD + E RGIT+K+
Sbjct: 15 NQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMKSSA 74
Query: 300 ASLNY---SYNNEK-----YLLNLIDT 356
SL + S N+EK YL+NLID+
Sbjct: 75 ISLFFKVISQNDEKRVEKDYLINLIDS 101
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 61.3 bits (142), Expect = 4e-11
Identities = 28/59 (47%), Positives = 40/59 (67%), Gaps = 1/59 (1%)
Frame = +3
Query: 135 IRNFSIVAHVDHGKSTLADRLLELTGVIKPG-ADHAQVLDQLQVERERGITVKAVTASL 308
+RN S++AHVDHGKSTL D L++ G+I A A+ +D E+ERG+T+K+ SL
Sbjct: 19 VRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISL 77
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 61.3 bits (142), Expect = 4e-11
Identities = 28/59 (47%), Positives = 40/59 (67%), Gaps = 1/59 (1%)
Frame = +3
Query: 135 IRNFSIVAHVDHGKSTLADRLLELTGVIKPG-ADHAQVLDQLQVERERGITVKAVTASL 308
+RN S++AHVDHGKSTL D L++ G+I A A+ +D E+ERG+T+K+ SL
Sbjct: 19 VRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISL 77
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 56.8 bits (131), Expect = 9e-10
Identities = 31/82 (37%), Positives = 52/82 (63%), Gaps = 3/82 (3%)
Frame = +3
Query: 120 INVKKIRNFSIVAHVDHGKSTLADRLLELTGVIK--PGADHAQ-VLDQLQVERERGITVK 290
I++ IRN I+AH+D GK+TL +++L G D V+D L ER+RGIT+
Sbjct: 23 ISINSIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITIN 82
Query: 291 AVTASLNYSYNNEKYLLNLIDT 356
+A++++++ N++ +NLIDT
Sbjct: 83 --SAAISFTWRNQR--INLIDT 100
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 49.6 bits (113), Expect = 1e-07
Identities = 26/62 (41%), Positives = 41/62 (66%), Gaps = 6/62 (9%)
Frame = +3
Query: 126 VKKIRNFSIVAHVDHGKSTLADRLLELTGVIKP-----GADHAQV-LDQLQVERERGITV 287
+K+IRN I AH+D GK+T +R+L TG IK G D+ +D +++ERE+GIT+
Sbjct: 56 LKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREKGITI 115
Query: 288 KA 293
++
Sbjct: 116 QS 117
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 36.7 bits (81), Expect = 0.001
Identities = 21/67 (31%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +3
Query: 141 NFSIVAHVDHGKSTLADRLLELTGVIKPGA--DHAQVLDQLQVERERGITVKAVTASLNY 314
N + HVDHGK+TL + + + + D++Q+ D+ E+ RGIT+ +A + Y
Sbjct: 55 NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQI-DKAPEEKARGITIS--SAHVEY 111
Query: 315 SYNNEKY 335
N Y
Sbjct: 112 ETANRHY 118
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 36.3 bits (80), Expect = 0.001
Identities = 23/77 (29%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
Frame = +3
Query: 135 IRNFSIVAHVDHGKSTLADRLLELT--GVIKPGADHAQVLDQLQVERERGITVKAVTASL 308
+R+F + H+ HGKS L D L+ T P + D +ERER +++K+ +L
Sbjct: 140 VRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMSIKSTPLTL 199
Query: 309 NYS-YNNEKYLLNLIDT 356
S + + IDT
Sbjct: 200 AVSDMKGKTFAFQCIDT 216
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 36.3 bits (80), Expect = 0.001
Identities = 28/87 (32%), Positives = 40/87 (45%), Gaps = 16/87 (18%)
Frame = +3
Query: 141 NFSIVAHVDHGKSTLADRLL----------------ELTGVIKPGADHAQVLDQLQVERE 272
N ++ HVD GKST L+ E T + K +A VLD+L+ ERE
Sbjct: 9 NVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Query: 273 RGITVKAVTASLNYSYNNEKYLLNLID 353
RGIT+ + + KY + +ID
Sbjct: 69 RGITIDIAL----WKFETPKYNVTVID 91
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 36.3 bits (80), Expect = 0.001
Identities = 28/87 (32%), Positives = 40/87 (45%), Gaps = 16/87 (18%)
Frame = +3
Query: 141 NFSIVAHVDHGKSTLADRLL----------------ELTGVIKPGADHAQVLDQLQVERE 272
N ++ HVD GKST L+ E T + K +A VLD+L+ ERE
Sbjct: 9 NVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Query: 273 RGITVKAVTASLNYSYNNEKYLLNLID 353
RGIT+ + + KY + +ID
Sbjct: 69 RGITIDIAL----WKFETPKYNVTVID 91
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 36.3 bits (80), Expect = 0.001
Identities = 28/87 (32%), Positives = 40/87 (45%), Gaps = 16/87 (18%)
Frame = +3
Query: 141 NFSIVAHVDHGKSTLADRLL----------------ELTGVIKPGADHAQVLDQLQVERE 272
N ++ HVD GKST L+ E T + K +A VLD+L+ ERE
Sbjct: 9 NVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Query: 273 RGITVKAVTASLNYSYNNEKYLLNLID 353
RGIT+ + + KY + +ID
Sbjct: 69 RGITIDIAL----WKFETPKYNVTVID 91
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 31.5 bits (68), Expect = 0.040
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 141 NFSIVAHVDHGKSTLADRLLELTGVI 218
N + HVD GKSTL +L LTG++
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 30.7 bits (66), Expect = 0.070
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 6/43 (13%)
Frame = +3
Query: 99 SEPSSKTINVKKIRN------FSIVAHVDHGKSTLADRLLELT 209
S PS K++N KK +++ HVDHGK+TL D + T
Sbjct: 153 SAPSPKSLNSKKDSGPLRPPVVTLMGHVDHGKTTLLDAFRKST 195
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 28.3 bits (60), Expect = 0.37
Identities = 21/77 (27%), Positives = 34/77 (44%), Gaps = 16/77 (20%)
Frame = +3
Query: 123 NVKKIRNFSIVAHVDHGKSTLADRLLELTGVI----------------KPGADHAQVLDQ 254
N K + + + HVD GKST+ R++ G I K +A +LD
Sbjct: 173 NPKPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDT 232
Query: 255 LQVERERGITVKAVTAS 305
+ ER RG+T+ + +
Sbjct: 233 TEEERARGVTMDVASTT 249
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.4 bits (53), Expect = 2.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 32 KHTFMLLYFRIPVQIFIPANVQFRTKF 112
KHT +PV+IF+P++ F + F
Sbjct: 313 KHTEWTSQGELPVEIFLPSSYHFHSVF 339
>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 485
Score = 25.0 bits (52), Expect = 3.5
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = +3
Query: 21 NIFKSTHLCFYTSGFQSKYLFR 86
N+F+ST + GF++ YL+R
Sbjct: 70 NVFQSTVIVISLDGFRADYLYR 91
>SPCC1442.15c |cox18||mitochondrial inner membrane protein
Cox18|Schizosaccharomyces pombe|chr 3|||Manual
Length = 202
Score = 24.6 bits (51), Expect = 4.6
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 218 KTRCRSCTSFRSITGRTRKRNYC 286
K RCR+ T FR++ + KR C
Sbjct: 55 KKRCRTNTDFRTVRKKLYKRFNC 77
>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 230
Score = 24.2 bits (50), Expect = 6.1
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +3
Query: 9 VKLINIFKSTHLCFYTSGFQSKYLFRRMCSSEPSSKTINVKKI 137
+ LIN ++LC +++ S +LF + P+S +IN KK+
Sbjct: 44 IPLINTH--SNLCLFSNFSHSCFLFCTHPDTLPTSLSINPKKL 84
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 24.2 bits (50), Expect = 6.1
Identities = 9/34 (26%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +3
Query: 216 IKPGADH-AQVLDQLQVERERGITVKAVTASLNY 314
IK AD ++++ + +ER+R + + ++ ++NY
Sbjct: 739 IKEAADRWSKLVTEKSIERKRNLAISSLNLAVNY 772
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 24.2 bits (50), Expect = 6.1
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +2
Query: 212 SYKTRCRSCTSFRSITGRTRKRNYCKSSYS 301
+Y R RSCTS R + K SYS
Sbjct: 54 AYHERLRSCTSCIQAYYELRNESLAKGSYS 83
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 23.8 bits (49), Expect = 8.0
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = +3
Query: 117 TINVKKIRNFSIVAHVDHGKSTLADRLLELTGVIKPGADHAQVLDQLQV 263
++N+K IV GKSTL L L +I+P + Q LD + +
Sbjct: 1245 SVNIKPQEKIGIVGRTGAGKSTLT---LALFRLIEPTSGDIQ-LDDINI 1289
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,435,837
Number of Sequences: 5004
Number of extensions: 27421
Number of successful extensions: 91
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 107972554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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