BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_P05
(471 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.12c |||NADH-cytochrome reductase|Schizosaccharomyces po... 29 0.27
SPAC4D7.09 |tif223||translation initiation factor eIF2B|Schizosa... 27 1.1
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 27 1.9
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 27 1.9
SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo... 27 1.9
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 25 4.4
SPAC694.03 |||conserved fungal protein|Schizosaccharomyces pombe... 25 7.7
SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase ... 25 7.7
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 25 7.7
>SPAC17H9.12c |||NADH-cytochrome reductase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 266
Score = 29.5 bits (63), Expect = 0.27
Identities = 21/76 (27%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +1
Query: 73 AVQIQEAYA-YKMNVNFLASGANNVGVGSAGSGIYSGKAGSLISIMPGVPTTRLLVSRVP 249
++Q+Q Y ++ N L G S I+S K G I + TT+L ++ P
Sbjct: 86 SMQVQRPYTPLYLDANELKFFIRKYEEGPVSSYIHSKKEGDTIELRGPFKTTKLDCTKYP 145
Query: 250 KVPGQVSGESYPGPIY 297
++ V+G PIY
Sbjct: 146 RIVAIVAGTGI-APIY 160
>SPAC4D7.09 |tif223||translation initiation factor
eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 468
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/48 (27%), Positives = 22/48 (45%)
Frame = +1
Query: 58 LPYLTAVQIQEAYAYKMNVNFLASGANNVGVGSAGSGIYSGKAGSLIS 201
+PYL Q Q+++ + N+ S NN+ G K +LI+
Sbjct: 277 IPYLVKCQYQKSFTVRENIQRFLSSPNNIDNYDGGLSSQEIKINALIA 324
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 26.6 bits (56), Expect = 1.9
Identities = 21/83 (25%), Positives = 35/83 (42%)
Frame = +2
Query: 215 FQLHGYLSQEYLKFQGRYLENHTQVQSTITRRIMXXXXXXXXXXXXXXSADLWYRDPKSL 394
F +H Y+ Q LKFQ + E+H + + ++ + +L Y DPK L
Sbjct: 677 FYVHPYIKQGILKFQSK--ESHKFRKKEVWSTVLAVLQRDVFTLYNLNTPNLSY-DPKDL 733
Query: 395 H*WIKMSAAILKSDSTKPEATPF 463
K+ ++K+ A PF
Sbjct: 734 D-ISKVGKPVIKTTIIASLAKPF 755
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 26.6 bits (56), Expect = 1.9
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = -1
Query: 213 SWHDADQRTCFTGIYSRTCRSDTNVIGPRSQKVHVHFVRIC 91
S+ D +T +SRT R + + P+S + V + RIC
Sbjct: 385 SYSDLRFELLYTESHSRTLRHEKRHVSPKSPLIDVCWWRIC 425
>SPBC27.02c |ask1|mug181|DASH complex subunit
Ask1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 26.6 bits (56), Expect = 1.9
Identities = 13/43 (30%), Positives = 19/43 (44%)
Frame = +1
Query: 265 VSGESYPGPIYDNPSDHDGLRLITDPSLVSHISRPLVQGSQEF 393
+SG P P+ NPSD D + T+ L H ++ F
Sbjct: 69 LSGVEEPVPVESNPSDQDVMSNSTEADLQLHTKNEHLEKRHSF 111
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 25.4 bits (53), Expect = 4.4
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +1
Query: 136 NNVGVGSAGSGIYSGKAGSLIS 201
NN+G GSAGSG++S G+ +S
Sbjct: 286 NNMG-GSAGSGVWSSCFGNSLS 306
>SPAC694.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 249
Score = 24.6 bits (51), Expect = 7.7
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -1
Query: 186 CFTGIYSRTCRSDTNVIGPRSQKVHVHF 103
C ++ CRS +N +GPR Q V F
Sbjct: 103 CKHALFVDKCRSISNKLGPREQVYLVGF 130
>SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase
Atp23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 185
Score = 24.6 bits (51), Expect = 7.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 105 NERELSGFWGQ*RWCRICRFGNIFR*SRFFDQHH 206
+E S G+ RW + RFGNI + F +HH
Sbjct: 110 SEIRASSMSGECRWTKELRFGNI----KTFRKHH 139
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 24.6 bits (51), Expect = 7.7
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 184 LYRNIFPNLQIRHQRYWPQKPES 116
L+R+++ N + Q YWP PES
Sbjct: 491 LHRDLYVN--VLSQAYWPSYPES 511
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,183,898
Number of Sequences: 5004
Number of extensions: 47985
Number of successful extensions: 137
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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