Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_O19
(605 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.06 |gln1||glutamate-ammonia ligase Gln1|Schizosaccharom... 79 4e-16
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 29 0.70
SPAC1527.02 |sft2||Golgi transport protein Sft2 |Schizosaccharom... 27 1.6
SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual 26 4.9
SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein Ucp7|Schizosaccha... 26 4.9
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 25 6.5
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 25 6.5
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 25 8.6
SPAC23C4.16c |atg15||triacylglycerol lipase Atg15 |Schizosacchar... 25 8.6
>SPAC23H4.06 |gln1||glutamate-ammonia ligase
Gln1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 359
Score = 79.4 bits (187), Expect = 4e-16
Identities = 44/79 (55%), Positives = 52/79 (65%), Gaps = 1/79 (1%)
Frame = +2
Query: 245 VLSKT-LLGRYNDLPLPADKIIATYIWIDGTGEHLRCKDRTLNFIPCMPKNLPVWNFDGS 421
+LSK +L +Y DLP K++A YIWIDG HLR K TL+ P L VWNFDGS
Sbjct: 9 LLSKAAILNKYADLPQNG-KVMAEYIWIDGFN-HLRSKTMTLDAKPSSIDQLRVWNFDGS 66
Query: 422 STGQADGHNSDTFLVPRAI 478
STGQA G+NSDT L P A+
Sbjct: 67 STGQAPGNNSDTLLKPVAM 85
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +3
Query: 471 VQFYKDPFRRGNHILVMCDTY*YNMETTESNHRIRCPEGYDRCTD 605
V Y DPFRRG++ILV+ Y + NHR C + ++ D
Sbjct: 83 VAMYNDPFRRGDNILVLAACYTADGSPNGFNHRDACAKLLEKHAD 127
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 28.7 bits (61), Expect = 0.70
Identities = 15/54 (27%), Positives = 27/54 (50%)
Frame = +2
Query: 167 SDPAHAKIEDNPKILSGPVLTNSPNAVLSKTLLGRYNDLPLPADKIIATYIWID 328
S P + ++DN + S +S N+ LS + G + +P+PA + T + D
Sbjct: 288 SKPNLSTLQDNASLTSQGSNLSSQNSGLSSSSSGIFGRMPIPAQSLDTTMLRTD 341
>SPAC1527.02 |sft2||Golgi transport protein Sft2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 27.5 bits (58), Expect = 1.6
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = -1
Query: 590 IALWTSNAVIAFCGFHVVLICVAHNKNMISTSKRIFIKL 474
+ LWT +++A GF +V VAH + ++T +R+ I L
Sbjct: 100 VLLWTMGSLLAVLGFAIVQGFVAHFR-QLTTMERLPITL 137
>SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual
Length = 264
Score = 25.8 bits (54), Expect = 4.9
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +3
Query: 429 VKRTAITLTPS*YHVQFYKDPFRRGNHILVM 521
VK ++L PS Y+ + YK G H++++
Sbjct: 34 VKTNYVSLKPSSYYAELYKLNHLEGAHVIML 64
>SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein
Ucp7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 697
Score = 25.8 bits (54), Expect = 4.9
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 179 HAKIEDNPKILSGPVLTNSPNAVLSKTLLGRYNDLPLPA 295
H KIE+N + +S P ++ S L L + + LPA
Sbjct: 116 HDKIEENKRPISQPQVSASEKVTLKDLSLEPHQPVSLPA 154
>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 897
Score = 25.4 bits (53), Expect = 6.5
Identities = 12/48 (25%), Positives = 26/48 (54%)
Frame = -3
Query: 357 SLHLRCSPVPSIHIYVAIILSAGSGKSLYRPSKVFESTALGELVNTGP 214
++++ SP P++ V I +A + + +PS F++ L ++T P
Sbjct: 234 NVNVDLSPFPNLPATVPITQAASTANAFQQPSNQFQTQKLPSGLDTRP 281
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 25.4 bits (53), Expect = 6.5
Identities = 20/85 (23%), Positives = 37/85 (43%), Gaps = 6/85 (7%)
Frame = +2
Query: 161 IMSDPAHAKIEDNPKILSGPVLTNSPNAVLSKT-LLGRYNDLPLPADKI-----IATYIW 322
+ S P HA+++ ++ S N+P VL T + R D+P I ++
Sbjct: 414 VPSYPLHAQLDQKKRLQSLEKFKNNPKGVLVCTDVAARGIDIPSVTHVIHYHVPHTADMY 473
Query: 323 IDGTGEHLRCKDRTLNFIPCMPKNL 397
+ +G R + ++ + C PK L
Sbjct: 474 VHRSGRTARANEDGVSILMCGPKEL 498
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/26 (46%), Positives = 14/26 (53%), Gaps = 2/26 (7%)
Frame = -3
Query: 603 LCID--HSPLDIECGDCFLWFPCCIN 532
LC++ H P ECG F W CIN
Sbjct: 258 LCMEFIHCPAATECGHIFCW--SCIN 281
>SPAC23C4.16c |atg15||triacylglycerol lipase Atg15
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 25.0 bits (52), Expect = 8.6
Identities = 14/49 (28%), Positives = 27/49 (55%)
Frame = -1
Query: 557 FCGFHVVLICVAHNKNMISTSKRIFIKLHVVLRRCQSYGRPLDPSNYHR 411
FC F + + C +++I +S+ + K++V L+ YG D +Y+R
Sbjct: 14 FC-FIIRISCTGVFESVIKSSENVPDKVNVKLQHVFHYGLNEDSISYYR 61
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,576,774
Number of Sequences: 5004
Number of extensions: 55210
Number of successful extensions: 114
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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