Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_O06
(579 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 225 3e-60
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 198 4e-52
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 97 2e-21
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.092
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 30 0.21
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.28
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 29 0.37
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.1
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.5
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 2.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.6
SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pomb... 25 6.1
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 6.1
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 225 bits (551), Expect = 3e-60
Identities = 113/154 (73%), Positives = 131/154 (85%)
Frame = +2
Query: 62 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 241
P+Y PFFGVMG +AI+F+S GAAYGTAK+G GI+AM V+RP+ I+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 242 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 421
YGLVV+VLI+G+L Q +LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQ
Sbjct: 67 YGLVVSVLISGNLKQI---LSLYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQ 123
Query: 422 PRLFVGMILILIFAEVLGLYRLIVAIYLYTEAVN 523
PRLFV MILILIFAEVLGLY LIVA+ L T A +
Sbjct: 124 PRLFVAMILILIFAEVLGLYGLIVALLLNTRATD 157
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 198 bits (484), Expect = 4e-52
Identities = 94/154 (61%), Positives = 120/154 (77%)
Frame = +2
Query: 62 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 241
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE +MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 242 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 421
YGLV++VLIAG + P N+Y+L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q
Sbjct: 67 YGLVMSVLIAGDMS-PDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQ 125
Query: 422 PRLFVGMILILIFAEVLGLYRLIVAIYLYTEAVN 523
R+FV M+LILIFAEVLGLY LIV + L T+ N
Sbjct: 126 DRIFVSMVLILIFAEVLGLYGLIVGLILQTKTSN 159
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 96.7 bits (230), Expect = 2e-21
Identities = 50/147 (34%), Positives = 80/147 (54%), Gaps = 5/147 (3%)
Frame = +2
Query: 80 FGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIYGLVVA 259
+G++G AS + F +GAA+G GT I AV P K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 260 VLIAGSLD--QPSNNYT---LYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 424
++ + ++ P+ YT Y GF G+ VG L G +GI G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 425 RLFVGMILILIFAEVLGLYRLIVAIYL 505
LFV ++++ IF VLGL+ LIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.092
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = -1
Query: 477 RPNTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTARPAPK*MNP 313
RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1693 RPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 30.3 bits (65), Expect = 0.21
Identities = 31/101 (30%), Positives = 47/101 (46%), Gaps = 2/101 (1%)
Frame = +2
Query: 248 LVVAVLIAGSLDQPSNNYTLYK--GFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 421
L+ + ++ + +N +Y+ G + +A+G +GLA G IG+ G G A
Sbjct: 141 LITSSMLLDTFQTMESNQEMYELSGTRKMRRRIAMGLAGLAGGALIGLTG-----GLAAP 195
Query: 422 PRLFVGMILILIFAEVLGLYRLIVAIYLYTEAVNQPEHTQL 544
FV L +FA LGL +I A YL T + P T L
Sbjct: 196 ---FVAAGLGTLFAG-LGLGTMIGATYLGTLITSAPMITAL 232
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 29.9 bits (64), Expect = 0.28
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = -1
Query: 486 SRYRPNTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTAR 337
S Y+P++S+K+ + + G A+P+ P P P +P R T +
Sbjct: 401 SSYKPSSSSKVSAKSVSRKPTGAPAIPKLP--PKHPSRQPTVRATPSTGK 448
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 29.5 bits (63), Expect = 0.37
Identities = 21/69 (30%), Positives = 30/69 (43%)
Frame = +2
Query: 206 IIPVVMAGIIAIYGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGI 385
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVI-------AAMVGILYFGHWLVYKILILAFGFKIVT 245
Query: 386 VGDAGVRGT 412
GD V T
Sbjct: 246 SGDVCVSNT 254
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.1
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = -1
Query: 414 AVPRTPAS--PTMPMAKPAARPEKPTARPAP 328
A P P S P++PM PAA P P+A AP
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.5 bits (58), Expect = 1.5
Identities = 16/80 (20%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +2
Query: 77 FFG-VMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMR-PEQIMKSIIPVVMAGIIAIYGL 250
F+G ++G + F+ + + ++ G + I+ S+ P+V++ IIA +
Sbjct: 1439 FYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFF 1498
Query: 251 VVAVLIAGSLDQPSNNYTLY 310
+ +++ L S Y +Y
Sbjct: 1499 CLGIMLRPILGDRSKTYGVY 1518
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.1 bits (57), Expect = 2.0
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +2
Query: 65 IYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIY 244
+YGPF+ A+ FS+ Y +G G + ++ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 245 GLVVAVLIAGSL 280
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 4.6
Identities = 19/61 (31%), Positives = 24/61 (39%), Gaps = 10/61 (16%)
Frame = -1
Query: 486 SRYRPNTSAKMRIRIIPTNNLGCWAVPRT----------PASPTMPMAKPAARPEKPTAR 337
S RP TSA R I P + +P PA P P+ P + P P+A
Sbjct: 135 SELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAV 194
Query: 336 P 334
P
Sbjct: 195 P 195
>SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 287
Score = 25.4 bits (53), Expect = 6.1
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +3
Query: 36 HIQEKWPKIQSMDLSLELWGRRLPSSSAPLVLHMAQPSQAPVLPP 170
HI WP+ QS D E G L + A + + + ++ +L P
Sbjct: 62 HIATLWPETQSSDQENEEEGEELEDAVAKEINSLQKKNKKELLTP 106
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 6.1
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -1
Query: 414 AVPRTPASPTMPMA----KPAARPEKPTARPAPK 325
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,794,475
Number of Sequences: 5004
Number of extensions: 64347
Number of successful extensions: 188
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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