Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_M21
(603 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 105 4e-24
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 67 2e-12
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 64 1e-11
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 63 3e-11
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 35 0.011
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 34 0.014
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 33 0.042
SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces ... 28 1.2
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 28 1.2
SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase ... 27 1.6
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 27 2.8
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 27 2.8
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 26 3.7
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.9
SPAC17A5.16 |||human down-regulated in multiple cancers-1 homolo... 26 4.9
SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces po... 25 6.4
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 25 8.5
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 25 8.5
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 105 bits (253), Expect = 4e-24
Identities = 66/199 (33%), Positives = 106/199 (53%), Gaps = 2/199 (1%)
Frame = +2
Query: 2 AVPPIVVFLAKAPIVAKYDLSSVNVVWCGAAPLSAETIN--ETLKRMPNCIGIFQAYGMT 175
AVP I L + P L + + ++PL+ ++ E R P + +AY MT
Sbjct: 256 AVPTIHQILLRTP--PPKPLPRIRFIRSCSSPLAPPVLSKLEATFRAP----VLEAYAMT 309
Query: 176 ETSLAATKDSDDANAVRKPGSGGYPLPGVKVKVVDITSRQKLGPNQEGEICIKGPIVMKG 355
E S T + V KP S G P GV++K++D + + +EGEIC++G V KG
Sbjct: 310 EASHQMTTNPLPP-LVHKPHSVGKPF-GVELKILDQKGNE-MPQGKEGEICVRGINVTKG 366
Query: 356 YAGDEKASRETMDEEGYLKTGDIGYYDKDGCFFIVDRLKELIKYKGYQVAPALVESVILQ 535
Y + A++ + ++ + +TGD G DKDG FI R+KEL+ G +++PA +++V++Q
Sbjct: 367 YLNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDAVLMQ 426
Query: 536 HSGVAECGVVGAPDENVGE 592
H V+E PDE G+
Sbjct: 427 HPDVSEAVCFAVPDEKYGQ 445
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 66.9 bits (156), Expect = 2e-12
Identities = 52/190 (27%), Positives = 86/190 (45%), Gaps = 9/190 (4%)
Frame = +2
Query: 50 KYDLSSVNVVWCGAAPLSAETINETLKRM--PNCIGIFQAYGMTETSLAATKDSDDANAV 223
K+D SS+ V+ P++ E+ + + C + Y TET +
Sbjct: 382 KHDRSSLRVLGSVGEPIAPESFMWYYEVVGEKRC-AVADTYWQTETGSHIVTSLGPVTPM 440
Query: 224 RKPGSGGYPLPGVKVKVVD-ITSRQKLGPNQEGEICIKGP------IVMKGYAGDEKASR 382
KPGS P G+ ++D +T + G + EG + I+ P V +G+ D
Sbjct: 441 -KPGSATLPFFGIDAVIIDPLTGKIIEGNDVEGVLAIRSPWPSAARTVWRGH--DRYIDT 497
Query: 383 ETMDEEGYLKTGDIGYYDKDGCFFIVDRLKELIKYKGYQVAPALVESVILQHSGVAECGV 562
G+ TGD DKDG +I R+ +++ G++++ A +E+ +L H VAE V
Sbjct: 498 YLKPYPGFYFTGDGATRDKDGYIWIRGRVDDVVNISGHRLSTAEIEAALLSHDAVAESAV 557
Query: 563 VGAPDENVGE 592
VG DE G+
Sbjct: 558 VGVHDELTGQ 567
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 64.5 bits (150), Expect = 1e-11
Identities = 40/123 (32%), Positives = 63/123 (51%), Gaps = 3/123 (2%)
Frame = +2
Query: 164 YGMTETSLAATKDSDDANAVRKPGSGGYPLPGVKVKVVDIT--SRQKLGPNQEGEICIKG 337
YG+TE S AA + S G LP ++K+VD + G GEI ++G
Sbjct: 433 YGLTEISAAAMVQNPAC--FNLDDSAGSLLPCTEMKLVDCEEGNYNSHGHPPRGEIWLRG 490
Query: 338 PIVMKGYAGDEKASRETMDEEGYLKTGDIGYYDKDGCFFIVDRLKELIK-YKGYQVAPAL 514
P + +GY +K ++E+ +G+ +TGD+G +G I+DR K L+K G +A
Sbjct: 491 PSLTRGYLNRDKENKESFTPDGWFRTGDVGELTPEGLLRIIDRKKNLVKTQNGEYIALEK 550
Query: 515 VES 523
+ES
Sbjct: 551 LES 553
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 62.9 bits (146), Expect = 3e-11
Identities = 38/134 (28%), Positives = 67/134 (50%), Gaps = 4/134 (2%)
Frame = +2
Query: 134 MPNCI-GIFQAYGMTETSLAATKDSDDANAVRKPGSGGYPLPGVKVKVVDITSRQKLGPN 310
+ +CI + YG+TET + S + + + G+P+P ++ K+VDI +
Sbjct: 428 LSSCICPVLPGYGLTETCAGSFVLSPEQWHLYA-NTVGFPIPSIEFKLVDIPDLGYYTDS 486
Query: 311 Q--EGEICIKGPIVMKGYAGDEKASRETMDEEGYLKTGDIGYYDKDGCFFIVDRLKELIK 484
GE+ I+GP V GY + ++ E+G+ KTGD+G K ++DR K ++K
Sbjct: 487 SPPRGEVWIRGPAVCNGYLNRPEDNKAAFTEDGWFKTGDVGEIAKGNTLRLIDRKKNIVK 546
Query: 485 -YKGYQVAPALVES 523
G +A +E+
Sbjct: 547 SLNGEYIALEKIEA 560
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 34.7 bits (76), Expect = 0.011
Identities = 46/171 (26%), Positives = 69/171 (40%), Gaps = 12/171 (7%)
Frame = +2
Query: 107 ETINETLKRMPNCIGIFQAYGMTETSLAATKDS--DDANAVRKPGSGGYPLPGVKVKVVD 280
E + E L ++ I + + G +E + KD D NAV+ S G LPG V
Sbjct: 395 EKLQERLAKLSGGIAVIKVGGSSEVEVNEKKDRIVDALNAVKAAVSEGV-LPGAGTSFVK 453
Query: 281 ITSRQKLGPNQEGEICIKGPIVMKGYAGDEKASRETMDEEGYLKTGDIG-YYDKD---GC 448
+ R P + + IV K + E EG L G + Y K+ G
Sbjct: 454 ASLRLGDIPTNNFDQKLGVEIVRKAITRPAQTILENAGLEGNLIVGKLKELYGKEFNIGY 513
Query: 449 FFIVDRLKELIKYKGYQVAPALVESVILQHSGVA------ECGVVGAPDEN 583
DR +L + G +V + ++ SGVA EC +V AP+E+
Sbjct: 514 DIAKDRFVDLNEI-GVLDPLKVVRTGLVDASGVASLMGTTECAIVDAPEES 563
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 34.3 bits (75), Expect = 0.014
Identities = 37/147 (25%), Positives = 61/147 (41%), Gaps = 4/147 (2%)
Frame = -1
Query: 603 AVGSSPTFSSGAPT-TPHSATPL-CCNITLSTNAGAT*YPLYLINSFNLSTIKKQP--SL 436
+V S+P S+ + T T S+TPL N T +T+A +T PL +NS ++ P S+
Sbjct: 419 SVSSTPLSSANSTTATSASSTPLSSVNSTTATSASST--PLSSVNSTTATSASSTPLTSV 476
Query: 435 S*YPISPVLRYPSSSIVSRDAFSSPA*PFITIGPLIHISPS*FGPSFCRDVIXXXXXXXX 256
+ + P +S+ S A S+ + P + S S PS+ +
Sbjct: 477 NSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTSSVLPTSSVSS 536
Query: 255 XXXXXXXXXFRTAFASSESLVAANDVS 175
T+ ASS L + N +
Sbjct: 537 TPLSSANSTTATS-ASSTPLTSVNSTT 562
Score = 25.8 bits (54), Expect = 4.9
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = -1
Query: 603 AVGSSPTFSSGAPT-TPHSATPLCC-NITLSTNAGAT*YPLYLINSFNLSTIKKQPSLS 433
+ S+P S + T T S+TPL N T +T+A +T PL +NS + ++ P S
Sbjct: 337 STSSTPLSSVNSTTATSASSTPLTSVNSTTATSASST--PLTSVNSTSATSASSTPLTS 393
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 32.7 bits (71), Expect = 0.042
Identities = 35/137 (25%), Positives = 58/137 (42%), Gaps = 13/137 (9%)
Frame = +2
Query: 152 IFQAYGMTETSLAATKDSDDANAVRKPGSGGYPLPGVKVKVVDITSRQK----LGPNQEG 319
+ AYG TE ++ T + + A+ G PL + VV + + L G
Sbjct: 499 LVNAYGPTEAAIHVTLNP--SKALTTVYLVGVPLQSATICVVSLPTEDSQPHVLHEGFLG 556
Query: 320 EICIKGPIVMKGYAGDEKASRETMDEEGY-------LKTGDIGYYDKDGCFFIVDRL--K 472
E+CI GP + GY + + + E Y +TGD+G +G +I R+
Sbjct: 557 EVCIAGPQLSSGYINRPEINAKAFVEVQYNEQTLSIYRTGDLGRI-INGKLYIFGRIAGD 615
Query: 473 ELIKYKGYQVAPALVES 523
IK +G ++ +ES
Sbjct: 616 MQIKIRGRRIEIGEIES 632
Score = 31.9 bits (69), Expect = 0.074
Identities = 34/137 (24%), Positives = 56/137 (40%), Gaps = 7/137 (5%)
Frame = +2
Query: 152 IFQAYGMTETSLAATKDSD-DANAVRKPGSGGYPLPGVKVKVVDITSRQKLGPNQEGEIC 328
++ +G TE ++ A+ NA K + G P P ++ ++ + GEIC
Sbjct: 3007 LYNFWGPTELTIGASAFRKVPKNA--KVSNIGPPFPNCSTYILSKETKVPVLLGGLGEIC 3064
Query: 329 IKGPIVMKGYAG-----DEKASRETMDEEGYLKTGDIGYYDKD-GCFFIVDRLKELIKYK 490
+ G V KGY D K + + TGD+G KD R + IK +
Sbjct: 3065 MGGNQVAKGYLNLPEQTDAKFYFDRRFNDRIYHTGDLGRLLKDNNSLEFCGRTDDQIKLR 3124
Query: 491 GYQVAPALVESVILQHS 541
G ++ + +VI S
Sbjct: 3125 GQRIEIGEINAVIKSSS 3141
Score = 27.1 bits (57), Expect = 2.1
Identities = 17/61 (27%), Positives = 25/61 (40%)
Frame = +2
Query: 242 GYPLPGVKVKVVDITSRQKLGPNQEGEICIKGPIVMKGYAGDEKASRETMDEEGYLKTGD 421
G PL G+ VV+ + GE+CI + G + T + Y +TGD
Sbjct: 2051 GIPLKGLIFAVVNSDTNTLAPIGSSGELCI-ASVKKSGNTMMDSQRVFTFENRSYYRTGD 2109
Query: 422 I 424
I
Sbjct: 2110 I 2110
>SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 512
Score = 27.9 bits (59), Expect = 1.2
Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = +2
Query: 452 FIVDRLKELIKYKGYQVAPALVESVILQHSGVAE-CGVVGAP 574
+ D ++E +YK ++++PA V I+ +GVA+ G V AP
Sbjct: 455 YAFDYIEERREYKHWKISPAGVPEEIIISNGVAQPTGNVAAP 496
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 27.9 bits (59), Expect = 1.2
Identities = 27/97 (27%), Positives = 44/97 (45%), Gaps = 5/97 (5%)
Frame = -1
Query: 594 SSPTFSSGAPTTPHSATPLCCNITLSTNAGAT*YPLYLINSFNLSTIKKQPSLS*--YPI 421
S+ T PT ++ T + N+T S + +T + ++ K PS S P
Sbjct: 259 SASTSPRNTPTPSNNGTSINANVTSSLTSNSTGKTSKTTDLLIAASKKSLPSNSTPSKPN 318
Query: 420 SPVLRYPSSSIV-SRD--AFSSPA*PFITIGPLIHIS 319
+ P ++I SRD AFS+P PF +G H++
Sbjct: 319 TSFFETPHNNIWDSRDRGAFSAPPAPFFPLGFSPHLN 355
>SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase
Ppk6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 27.5 bits (58), Expect = 1.6
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -3
Query: 394 VHCLSGRFLVTSITFHNYWSFDTYFTFL 311
VHC S + TS T + WS + YF L
Sbjct: 151 VHCSSQAIVTTSNTTFSIWSANDYFCLL 178
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 26.6 bits (56), Expect = 2.8
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = +2
Query: 191 ATKDSDDANAVRKPGSGGYPLPGVKVKVVDITSRQKLGPNQEGEICIKGPIVMKGY 358
A + S N +R G+ YP + +VD ++ PN GEI + P + G+
Sbjct: 575 ARRQSKHPNTIRV-GAFWYPFVDATLAIVDPETQVLCLPNIVGEIWVDSPSLSGGF 629
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1428
Score = 26.6 bits (56), Expect = 2.8
Identities = 25/113 (22%), Positives = 49/113 (43%), Gaps = 14/113 (12%)
Frame = +2
Query: 245 YPLPGVKVKVVDITSRQKLGPNQEGEI-----C---IKGPIVMKGYAGDEKASRETMDE- 397
YP+P V VV+ S+ + GEI C +K V+ G++ E E
Sbjct: 508 YPIPEASVAVVNEDSKNICKVGEVGEIWVYSDCLPKLKAASVINNPQGEQLTDYENNQEN 567
Query: 398 -----EGYLKTGDIGYYDKDGCFFIVDRLKELIKYKGYQVAPALVESVILQHS 541
++ +G G+ + F + ++ +++ +Y+ VA L+E + +S
Sbjct: 568 KFEKNNSFMDSGLKGFLHNEKIFVLGNKDEQIRQYRKTMVADKLMEEKYVHYS 620
Score = 25.8 bits (54), Expect = 4.9
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +2
Query: 359 AGDEKASRETMDEEGYLKTGDIGY 430
+G E +ET+D++ Y +TG +G+
Sbjct: 1230 SGCELLVQETLDKKSYSRTGQLGF 1253
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 26.2 bits (55), Expect = 3.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 92 APLSAETINETLKRMPNCIGIFQAYGMTETSLAATKDS 205
A S + ++E + PN + IFQ + TSL+A KD+
Sbjct: 336 ASASVDDVSEESQVEPNTL-IFQNFSSPSTSLSAPKDT 372
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 4.9
Identities = 24/78 (30%), Positives = 38/78 (48%), Gaps = 1/78 (1%)
Frame = -1
Query: 594 SSPTFSSGAPTTPHSATPLCCNITLSTNAGAT*YPLYLI-NSFNLSTIKKQPSLS*YPIS 418
SS TFSS APT+ S+ ++ S+++ ++ L +S + S+I S S S
Sbjct: 193 SSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSS 252
Query: 417 PVLRYPSSSIVSRDAFSS 364
+ SSS S + SS
Sbjct: 253 SLSSSSSSSTASSSSSSS 270
>SPAC17A5.16 |||human down-regulated in multiple cancers-1 homolog
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 25.8 bits (54), Expect = 4.9
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -1
Query: 453 KKQPSLS*YPISPVLRYPSSSIVSRDAFSSPA*PFITIG 337
+K PS+ P+SPV +P+SSI S DA S + +++G
Sbjct: 161 QKSPSV---PLSPVSTFPASSI-SLDASSDVSAADVSVG 195
>SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 25.4 bits (53), Expect = 6.4
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -3
Query: 103 TEGSGTTPHDINRR*IVFRYNW 38
T G G P ++ R I +R+NW
Sbjct: 60 TTGQGEMPLNMRERIITYRFNW 81
>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 677
Score = 25.0 bits (52), Expect = 8.5
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -1
Query: 588 PTFSSGAPTTPHSATPLCCNITLSTNAGAT*YP 490
PT+S TT SATP + + ST G +P
Sbjct: 452 PTYSQSTSTTNPSATPTTGDPSNSTYGGPQTFP 484
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.0 bits (52), Expect = 8.5
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = -3
Query: 358 ITFHNYWSFDTYF-TFLIWSKFLSRCYIDYLHFHPW 254
+ F+ Y S D F T + L+RC IDY +H W
Sbjct: 2987 VVFYVYTSADVDFQTNSLSPALLNRCTIDY--YHSW 3020
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,532,688
Number of Sequences: 5004
Number of extensions: 52616
Number of successful extensions: 153
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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