Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_L20
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 90 2e-19
SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 32 0.060
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 30 0.24
SPAC17G8.11c |||mannosyltransferase complex subunit |Schizosacch... 25 5.2
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 25 5.2
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 25 6.8
SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 25 6.8
SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha... 25 9.0
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 25 9.0
SPAC3A11.09 |sod22||plasma membrane alkali metal cation/H+ antip... 25 9.0
SPAC806.08c |mod21||gamma tubulin complex subunit Mod21|Schizosa... 25 9.0
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 9.0
SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch... 25 9.0
SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar... 25 9.0
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.0
SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha... 25 9.0
>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
3-phosphatidyltransferase Pis1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 90.2 bits (214), Expect = 2e-19
Identities = 42/92 (45%), Positives = 61/92 (66%)
Frame = +2
Query: 224 SFYFMSTHCVLSCICYVASALLDAFDGHAARYFNQSTKFGAILDQLTDRAGTAGLMMTLA 403
S YFMS H I Y+ S+LLDAFDG AAR +Q+T FGAILD +TDR T+ L+ L
Sbjct: 44 SLYFMSWHPNYCTIVYLYSSLLDAFDGWAARKLHQATNFGAILDMVTDRCATSCLLCFLC 103
Query: 404 TFYPRYTFWLQISMAIDITCHWIYLHSSTLQG 499
YP+Y Q+ +++D+ H+++++S+ QG
Sbjct: 104 AAYPKYAIIFQLLVSLDLASHYMHMYSTLHQG 135
>SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 570
Score = 31.9 bits (69), Expect = 0.060
Identities = 23/84 (27%), Positives = 34/84 (40%), Gaps = 1/84 (1%)
Frame = +2
Query: 155 IKENIFLFVPNXXXXXXXXXXXXSFYFMSTHCVLSCICYVASA-LLDAFDGHAARYFNQS 331
++ENIF +PN Y + C+ A D DG+ AR F+
Sbjct: 394 LRENIFT-LPNLLTFSRLLSAPLIAYLYIYDYTKAAACFFLYAGFTDLVDGYIARKFDLG 452
Query: 332 TKFGAILDQLTDRAGTAGLMMTLA 403
+ G +LD L D+ L + LA
Sbjct: 453 SIAGTVLDPLADKTLMTCLTICLA 476
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 29.9 bits (64), Expect = 0.24
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -2
Query: 130 ECNAVQRMNKIIFTSLLMRMATCEMYTTDLLEKLK 26
+C++ R N I+ L R + Y TDLLE+LK
Sbjct: 703 QCSSTPRTNTILANERLPRSRRDDPYWTDLLERLK 737
>SPAC17G8.11c |||mannosyltransferase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 25.4 bits (53), Expect = 5.2
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +3
Query: 426 FGYKFPWLLILLATGSIF 479
F Y FP+L ++ +TG +F
Sbjct: 193 FNYHFPYLTVMYSTGPLF 210
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 25.4 bits (53), Expect = 5.2
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -2
Query: 346 GSKFSTLIEIPCSMPIKRIEQSRSNVAD 263
GSKF T ++ P M + + + +N+AD
Sbjct: 218 GSKFITFLDTPGHMAFEAMRKRGANIAD 245
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 25.0 bits (52), Expect = 6.8
Identities = 13/50 (26%), Positives = 23/50 (46%)
Frame = -2
Query: 169 NVFFNVSHLEIKGECNAVQRMNKIIFTSLLMRMATCEMYTTDLLEKLKHQ 20
N FF+ HL I+G+ + N I +L + ++ LL++ Q
Sbjct: 394 NYFFHAGHLHIEGQKMSKSLKNFITIKEILKKFTPRQLRLAFLLQQWNTQ 443
>SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 25.0 bits (52), Expect = 6.8
Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = -2
Query: 193 NNIRDK*KNVFFN-VSHLEI--KGECNAVQRMNK 101
N ++ KNV +N VS + I KG+ NAV MNK
Sbjct: 159 NAVKTIEKNVNYNNVSDIVIPNKGDANAVMHMNK 192
>SPBC1348.02 |||S. pombe specific 5Tm protein
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 344
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 240 LRIVYCRASATLLLLCSMRLMGMLQGISIKVLNLEPYWISL 362
L ++YC + LM ++GISI + YWISL
Sbjct: 181 LFVIYCALGFGITRTVPKYLM--IKGISIVIALCSVYWISL 219
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 24.6 bits (51), Expect = 9.0
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -2
Query: 367 VCKLIQYGSKFSTLIEIPCSMPIKRIEQSRSNV 269
VCK +Y F L PIK +E+ +NV
Sbjct: 543 VCKRPEYSDGFELLYGSALVSPIKSVERVTNNV 575
>SPAC3A11.09 |sod22||plasma membrane alkali metal cation/H+
antiporter Sod22|Schizosaccharomyces pombe|chr
1|||Manual
Length = 759
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -2
Query: 310 SMPIKRIEQSRSNVADARQYTMRRHKI 230
S+PIKR++ RS+ RRH +
Sbjct: 467 SLPIKRVDALRSSANSIASSIRRRHPV 493
>SPAC806.08c |mod21||gamma tubulin complex subunit
Mod21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 618
Score = 24.6 bits (51), Expect = 9.0
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = +2
Query: 101 FIHTLHCIALTFYLKMTDIKEN 166
F++T H LT Y T++ EN
Sbjct: 473 FVNTCHNFFLTLYQSFTEVDEN 494
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = -1
Query: 77 ENGDMRDVHHRLTREAKASDGELP 6
E+GD R+VHH T +D P
Sbjct: 728 ESGDAREVHHMCTLLGYGADAVCP 751
>SPBPB2B2.19c |||S. pombe specific 5Tm protein
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 344
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 240 LRIVYCRASATLLLLCSMRLMGMLQGISIKVLNLEPYWISL 362
L ++YC + LM ++GISI + YWISL
Sbjct: 181 LFVIYCALGFGITRTVPKYLM--IKGISIVIALCSVYWISL 219
>SPAC977.01 |||S. pombe specific 5Tm protein
family|Schizosaccharomyces pombe|chr 1||Partial|Manual
Length = 316
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 240 LRIVYCRASATLLLLCSMRLMGMLQGISIKVLNLEPYWISL 362
L ++YC + LM ++GISI + YWISL
Sbjct: 153 LFVIYCALGFGITRTVPKYLM--IKGISIVIALCSVYWISL 191
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 24.6 bits (51), Expect = 9.0
Identities = 8/29 (27%), Positives = 17/29 (58%)
Frame = +2
Query: 44 VCGVHLACRHSHKK*SENNFIHTLHCIAL 130
+CG ++ C H ++ +++ T HC A+
Sbjct: 276 ICG-NIGCGRYHDAHAKQHYVDTAHCYAM 303
>SPAC750.05c |||S. pombe specific 5Tm protein
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 344
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 240 LRIVYCRASATLLLLCSMRLMGMLQGISIKVLNLEPYWISL 362
L ++YC + LM ++GISI + YWISL
Sbjct: 181 LFVIYCALGFGITRTVPKYLM--IKGISIVIALCSVYWISL 219
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,075,718
Number of Sequences: 5004
Number of extensions: 40016
Number of successful extensions: 112
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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