BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_G06
(192 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 44 4e-06
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 29 0.085
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 27 0.26
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 0.79
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 1.8
SPMIT.05 |cob1|cob|cytochrome b, Cob1|Schizosaccharomyces pombe|... 24 2.4
SPBC13E7.11 ||SPBC30D10.19c|mitochondrial rhomboid protease|Schi... 23 4.2
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 23 4.2
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 23 5.6
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 23 5.6
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 43.6 bits (98), Expect = 4e-06
Identities = 21/62 (33%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +3
Query: 6 EAQKWIEAVIGERFPSDLPYELPLRNGIILCKLMNRLQPGLISKVNVSGG-DYXYMDHIH 182
EA+KWIE +G +E LRNG++L L+ + QP + K+ S + + D+I+
Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105
Query: 183 QF 188
+F
Sbjct: 106 KF 107
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 29.1 bits (62), Expect = 0.085
Identities = 16/62 (25%), Positives = 31/62 (50%)
Frame = +3
Query: 3 QEAQKWIEAVIGERFPSDLPYELPLRNGIILCKLMNRLQPGLISKVNVSGGDYXYMDHIH 182
+EA++WIE + + + L L++G+ILC++ G + S + M++I
Sbjct: 7 KEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEAL-GANIRYKESNMPFVQMENIS 65
Query: 183 QF 188
F
Sbjct: 66 AF 67
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 27.5 bits (58), Expect = 0.26
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +3
Query: 6 EAQKWIEAVIGERFPSDLPYELPLRNGIILCKLMNRLQPGLIS 134
EA+KW+E + + + L NG +LC+L + P L S
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLAS 111
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.8 bits (54), Expect = 0.79
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 30 VIGERFPSDLPYELPLRNGIILCKLMNRLQPGL 128
+IGERFP ++ + IIL K+ L+P L
Sbjct: 2293 IIGERFPVEVKCAILYTLNIILSKISTFLRPFL 2325
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 24.6 bits (51), Expect = 1.8
Identities = 9/33 (27%), Positives = 20/33 (60%)
Frame = +3
Query: 81 NGIILCKLMNRLQPGLISKVNVSGGDYXYMDHI 179
+ +I+C + RL+P + S + + GG + + D +
Sbjct: 2017 SAMIVCSYLIRLRPFVDSYLALQGGQFDHADRL 2049
>SPMIT.05 |cob1|cob|cytochrome b, Cob1|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 387
Score = 24.2 bits (50), Expect = 2.4
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = -3
Query: 127 NPGCSLFISLHRMMPFL 77
NP + F SLH +MPF+
Sbjct: 173 NPTLNRFFSLHYLMPFV 189
>SPBC13E7.11 ||SPBC30D10.19c|mitochondrial rhomboid
protease|Schizosaccharomyces pombe|chr 2|||Manual
Length = 298
Score = 23.4 bits (48), Expect = 4.2
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +3
Query: 57 LPYELPLRNGIILCKLMNRLQPGLISKVNVSGGDYXYMDH 176
LP+ +P++ G+ L LM GLIS+ S ++ +DH
Sbjct: 217 LPF-IPIKIGVALLGLMAFDAWGLISRGFSSFANFTLIDH 255
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 23.4 bits (48), Expect = 4.2
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 10 PKNGLKR**VNDSLQICP 63
PK+G R ND+L +CP
Sbjct: 173 PKSGFTRSFNNDTLMVCP 190
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 23.0 bits (47), Expect = 5.6
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -1
Query: 69 VHRANLKGIVHLSPLQS 19
V R N I+HL P+QS
Sbjct: 758 VQRRNAANILHLEPIQS 774
>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 885
Score = 23.0 bits (47), Expect = 5.6
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3
Query: 117 QPGLISKVNVSGGDYXYMDHI 179
Q GL++K+N S GD + I
Sbjct: 58 QKGLLAKLNASNGDVIWRQSI 78
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 812,668
Number of Sequences: 5004
Number of extensions: 12120
Number of successful extensions: 32
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32
length of database: 2,362,478
effective HSP length: 43
effective length of database: 2,147,306
effective search space used: 42946120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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