BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_F18
(472 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 93 3e-20
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 29 0.47
SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 4.4
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 25 5.8
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 7.7
>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
L22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 92.7 bits (220), Expect = 3e-20
Identities = 46/109 (42%), Positives = 64/109 (58%), Gaps = 1/109 (0%)
Frame = +2
Query: 131 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARD-KTKVAINADI 307
+S K+ ID T D I DV FEKYL +R+KV+GKT NLG+ VV++R+ +K+A+ A I
Sbjct: 8 VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIAHI 67
Query: 308 PFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDSYELRYFNINADSDNED 454
FS DWLRVV++ YELRY+N+ +D E+
Sbjct: 68 DFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRYYNVVVGNDEEE 116
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 28.7 bits (61), Expect = 0.47
Identities = 19/62 (30%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = -2
Query: 261 T*LPRLFVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPPFTP 85
T +P+L L +T + F++ R+ G ++++ L ++F +L L RI+ PFTP
Sbjct: 714 TVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTP 770
Query: 84 FL 79
F+
Sbjct: 771 FI 772
>SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1573
Score = 25.4 bits (53), Expect = 4.4
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +2
Query: 221 VKVEGKTNNLGNHVVIARDKTKVAINADIP 310
+K+ G NNL +++ + +T++ + DIP
Sbjct: 1210 IKLVGMANNLNDNLELEEFRTRITNSMDIP 1239
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 25.0 bits (52), Expect = 5.8
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 324 ISNT*PSVTSRKTIFGT-GSEWWLLHMTHMSFATS 425
I N TS K +FG G WW+L +T + T+
Sbjct: 180 ILNNGIKATSTKVLFGLLGLWWWMLFVTASFYHTT 214
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 24.6 bits (51), Expect = 7.7
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2
Query: 185 DVGNFEKYLKERVKVEGKTNNLGNH 259
+ GN +KY + +KV GK ++ H
Sbjct: 955 EAGNLKKYDQPNLKVSGKNDSFVTH 979
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,824,689
Number of Sequences: 5004
Number of extensions: 33979
Number of successful extensions: 91
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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