BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0003_E02
(437 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.41
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 2.2
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 2.9
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 25 3.9
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 25 3.9
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 25 5.1
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 6.7
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 6.7
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 25 6.7
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 6.7
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 24 8.9
SPAC12G12.05c |taf9||transcription initiation factor Taf9|Schizo... 24 8.9
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.41
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -3
Query: 99 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 19
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 26.2 bits (55), Expect = 2.2
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +1
Query: 157 PTNLSL-VSAPLDVRGSSIQTVKFPEFHRYT 246
PT L SA DVR +S + +FH+Y+
Sbjct: 51 PTELKARTSATFDVRSASTSPINASDFHKYS 81
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 2.9
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +3
Query: 75 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWK--LDSDSEIS 227
P PQ+ + +QN + + + G P K V+ + T DSDS++S
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSDSKLS 829
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 25.4 bits (53), Expect = 3.9
Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 5/63 (7%)
Frame = +3
Query: 117 DAAINYWIQNGAPTHKLVLGISTTGRTWKLDSDSEISGVPP-----IHADXGGEAGPYTK 281
+ A YWI+ T + +LG S + + + IS + P H+ G GP TK
Sbjct: 792 EKATTYWIKGTEFTVERLLGYSNEAQRF-VGGSICISRLAPQDYHRFHSPVNGCIGPITK 850
Query: 282 VQG 290
++G
Sbjct: 851 IEG 853
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 25.4 bits (53), Expect = 3.9
Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +3
Query: 282 VQGLLSYPEICAKLINPNQNGKRPHLRKVNDP-SKRFGTYAFRLPDDXGEGGF 437
V L+SYP++ ++ + + +LR++N P + F +A P EG F
Sbjct: 1815 VNSLISYPKMNIEVSQSSSDKLLLYLRRLNQPLNPDFYIFAPLFPKPQSEGFF 1867
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 25.0 bits (52), Expect = 5.1
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +3
Query: 240 IHADXGGEAGPYTKVQGLLSY 302
+H G AG Y KV+G + Y
Sbjct: 562 LHKKQSGGAGQYAKVEGYIEY 582
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 24.6 bits (51), Expect = 6.7
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +1
Query: 142 KMVRLPTNLSLVSAPLDVR---GSSIQTVKFPEFHRYTP 249
+++ +P NL+ PL GSS +T + PEF TP
Sbjct: 144 ELIMVPPNLA--GKPLSANSSFGSSAKTFQLPEFDSVTP 180
>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 713
Score = 24.6 bits (51), Expect = 6.7
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +3
Query: 42 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 161
+R+P + Y + P N +NA A+NY+ + A H
Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 24.6 bits (51), Expect = 6.7
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 290 TLNFGIRTSFTTLIGVYRWNSGNFTV*IE 204
T F + SFT+ +YRWN N V IE
Sbjct: 140 TFTFSDKPSFTS---IYRWNVTNSNVTIE 165
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 24.6 bits (51), Expect = 6.7
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +3
Query: 30 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 158
Y TP Y P Y P R+P Q+ A + NG PT
Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 24.2 bits (50), Expect = 8.9
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 303 PEICAKLINPNQNGKRPHLRKVNDPSKRFGTYAFRLP 413
PE+ + L P ++ L + PS +FG +F P
Sbjct: 66 PEVSSPLAEPYESSSSFRLSLSSPPSSKFGGPSFGTP 102
>SPAC12G12.05c |taf9||transcription initiation factor
Taf9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 163
Score = 24.2 bits (50), Expect = 8.9
Identities = 32/99 (32%), Positives = 41/99 (41%), Gaps = 1/99 (1%)
Frame = +1
Query: 130 ITGFKMVRL-PTNLSLVSAPLDVRGSSIQTVKFPEFHRYTPXRVVKLVLIPKFKVY*ATQ 306
I G K VRL LS + P + +Q + F HRYT LI +VY A
Sbjct: 11 IKGPKDVRLIHLILSSLGVPSYSQTVPLQLLTFA--HRYTQQ------LIQDSQVY-AEH 61
Query: 307 KFAPNLLTPTKTESVLIFVRSTILANVLGPMPSAFLMXM 423
N P E V + V S I + GP P FL+ +
Sbjct: 62 SRGQN--APISVEDVRLAVASQINHSFTGPPPKEFLLEL 98
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,912,865
Number of Sequences: 5004
Number of extensions: 39735
Number of successful extensions: 118
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 158122380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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