BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_O19
(364 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5; Obtectomera|... 105 2e-22
UniRef50_P50725 Cluster: Attacin-A precursor; n=14; Obtectomera|... 68 6e-11
UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria cunea... 61 7e-09
UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin ... 38 0.072
UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains: Immune-i... 36 0.22
UniRef50_Q8WQW8 Cluster: Serine proteinase inhibitor serpin-4; n... 33 2.1
UniRef50_Q22XV6 Cluster: Insect antifreeze protein; n=3; Eukaryo... 32 2.7
UniRef50_Q70I53 Cluster: Histone deacetylase-like amidohydrolase... 32 2.7
UniRef50_Q39M17 Cluster: Outer membrane protein; n=4; Burkholder... 32 3.6
UniRef50_Q6AEK3 Cluster: Lipopolysaccharide exporter; n=3; Actin... 31 6.3
UniRef50_O66895 Cluster: Hydrogenase large subunit; n=5; Bacteri... 31 6.3
UniRef50_Q1QKG4 Cluster: Putative uncharacterized protein precur... 31 6.3
UniRef50_Q2CEK6 Cluster: Putative uncharacterized protein; n=1; ... 31 8.3
UniRef50_Q8W2Y1 Cluster: Putative polyprotein protein; n=2; Oryz... 31 8.3
UniRef50_Q336X3 Cluster: Retrotransposon protein, putative, LINE... 31 8.3
>UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5;
Obtectomera|Rep: Attacin-like protein - Antheraea
mylitta (Tasar silkworm)
Length = 230
Score = 105 bits (253), Expect = 2e-22
Identities = 47/57 (82%), Positives = 53/57 (92%)
Frame = +2
Query: 194 SGAMVKVPITGNENHRLSALGSVDLTNQMKLGAATAGLAYDNVNGHGATLTKTHIPG 364
SGA VK+PITGNENH+LSA+GS+D ++ KLGAATAGLAYDNVNGHGATLTKTHIPG
Sbjct: 59 SGAAVKIPITGNENHKLSAIGSLDFNDRNKLGAATAGLAYDNVNGHGATLTKTHIPG 115
Score = 56.8 bits (131), Expect = 1e-07
Identities = 25/29 (86%), Positives = 28/29 (96%)
Frame = +3
Query: 84 KPGYYIEQYEDQPEQWANSRVGRRQAGAL 170
+PGYYIEQYE+QPEQW+NSRV RRQAGAL
Sbjct: 24 QPGYYIEQYEEQPEQWSNSRV-RRQAGAL 51
>UniRef50_P50725 Cluster: Attacin-A precursor; n=14;
Obtectomera|Rep: Attacin-A precursor - Trichoplusia ni
(Cabbage looper)
Length = 254
Score = 67.7 bits (158), Expect = 6e-11
Identities = 35/70 (50%), Positives = 45/70 (64%)
Frame = +2
Query: 155 ASGCTXLSTQTAXSGAMVKVPITGNENHRLSALGSVDLTNQMKLGAATAGLAYDNVNGHG 334
A G L++ + G KVPI GNE + LSALGSVDL +Q+K + GLA DNVNGHG
Sbjct: 64 AQGSVTLNSDGSM-GLGAKVPIVGNEKNVLSALGSVDLNDQLKPASRGMGLALDNVNGHG 122
Query: 335 ATLTKTHIPG 364
++ K +PG
Sbjct: 123 LSVMKETVPG 132
>UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria
cunea|Rep: Putative attacin - Hyphantria cunea (Fall
webworm)
Length = 233
Score = 60.9 bits (141), Expect = 7e-09
Identities = 29/56 (51%), Positives = 37/56 (66%)
Frame = +2
Query: 194 SGAMVKVPITGNENHRLSALGSVDLTNQMKLGAATAGLAYDNVNGHGATLTKTHIP 361
S A +K+P+ G+ + LSALGSV L +A+ GLA DNV GHG +LT THIP
Sbjct: 60 SRANIKLPLAGSNKNVLSALGSVGFDANKHLSSASGGLALDNVRGHGLSLTGTHIP 115
>UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin -
Musca domestica (House fly)
Length = 208
Score = 37.5 bits (83), Expect = 0.072
Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 3/48 (6%)
Frame = +2
Query: 227 NENHRLSALG---SVDLTNQMKLGAATAGLAYDNVNGHGATLTKTHIP 361
N+ H+L A +L N K GL Y++ NGHGA++T + IP
Sbjct: 106 NDKHKLDANAFHSRTNLDNGFKFNTVGGGLDYNHANGHGASVTASRIP 153
>UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains:
Immune-induced peptide 16 (DIM-16) (MPAC)]; n=21;
Sophophora|Rep: Attacin-C precursor [Contains:
Immune-induced peptide 16 (DIM-16) (MPAC)] - Drosophila
melanogaster (Fruit fly)
Length = 241
Score = 35.9 bits (79), Expect = 0.22
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = +2
Query: 266 LTNQMKLGAATAGLAYDNVNGHGATLTKTHIPG 364
L N K A L Y ++ GHGATLT +IPG
Sbjct: 155 LANGFKFDRNGAALDYSHIKGHGATLTHANIPG 187
Score = 31.1 bits (67), Expect = 6.3
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +2
Query: 299 AGLAYDNVNGHGATLTKTHIPG 364
A L Y+N +GHG LTKTH PG
Sbjct: 103 ATLGYNN-HGHGLELTKTHTPG 123
>UniRef50_Q8WQW8 Cluster: Serine proteinase inhibitor serpin-4; n=4;
Ixodidae|Rep: Serine proteinase inhibitor serpin-4 -
Rhipicephalus appendiculatus (Brown ear tick)
Length = 485
Score = 32.7 bits (71), Expect = 2.1
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = -2
Query: 234 FSFPVIGTLTIAPEXAV*VDXLVHPLAGXQPWSWPTA-PADPHIVQCSNQAFTSTYRL*P 58
F F ++ + ++P+ + P +G QPW WPT+ P + V + TS+ L P
Sbjct: 29 FGFKLLNAIPVSPQ----TNLFYSPYSGPQPWRWPTSVPGERLSVTFTRHWATSSVGLTP 84
Query: 57 D 55
+
Sbjct: 85 E 85
>UniRef50_Q22XV6 Cluster: Insect antifreeze protein; n=3;
Eukaryota|Rep: Insect antifreeze protein - Tetrahymena
thermophila SB210
Length = 8517
Score = 32.3 bits (70), Expect = 2.7
Identities = 17/55 (30%), Positives = 24/55 (43%)
Frame = +3
Query: 12 QTHKDVAKFLVSSFGRVTAGTCS*KPGYYIEQYEDQPEQWANSRVGRRQAGALXC 176
Q +DV F +S ++ C PGY++EQY DQ S + L C
Sbjct: 5978 QCSQDVTLFQPASDIKICTVQCDEYPGYFLEQYTDQQSLQVVSTCSKCAENCLSC 6032
>UniRef50_Q70I53 Cluster: Histone deacetylase-like amidohydrolase;
n=2; Proteobacteria|Rep: Histone deacetylase-like
amidohydrolase - Alcaligenes sp. (strain DSM 11172)
(Bordetella sp. (strain FB188))
Length = 369
Score = 32.3 bits (70), Expect = 2.7
Identities = 18/38 (47%), Positives = 23/38 (60%)
Frame = +2
Query: 224 GNENHRLSALGSVDLTNQMKLGAATAGLAYDNVNGHGA 337
G E RLSA G+V+LT ++ G +AG A N GH A
Sbjct: 107 GLEIARLSAGGAVELTRRVATGELSAGYALVNPPGHHA 144
>UniRef50_Q39M17 Cluster: Outer membrane protein; n=4; Burkholderia
cepacia complex|Rep: Outer membrane protein -
Burkholderia sp. (strain 383) (Burkholderia cepacia
(strain ATCC 17760/ NCIB 9086 / R18194))
Length = 388
Score = 31.9 bits (69), Expect = 3.6
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 155 ASGCTXLSTQTAXSGAMVKVPITG-NENHRLSALGSVDLTNQMKLGAATAGLAYDNVNGH 331
A+G T + Q + GA+ P +G N + + G + Q ++G TAGL Y N
Sbjct: 221 ANGWTNATAQPSFGGAL-GYPSSGYNGGNAFKSAGIAQVATQYQIGPYTAGLRYSNAQYQ 279
Query: 332 G 334
G
Sbjct: 280 G 280
>UniRef50_Q6AEK3 Cluster: Lipopolysaccharide exporter; n=3;
Actinobacteria (class)|Rep: Lipopolysaccharide exporter
- Leifsonia xyli subsp. xyli
Length = 294
Score = 31.1 bits (67), Expect = 6.3
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = -2
Query: 288 PNFIWLVRSTEPRALSL*FSFPVIGTLTIAPEXAV*VDXLVHPLA 154
P IW+ R + + S PV+G IA +D L+ PLA
Sbjct: 111 PRTIWVNRIVASKGIEFLLSLPVLGIFAIATGARANIDALLFPLA 155
>UniRef50_O66895 Cluster: Hydrogenase large subunit; n=5;
Bacteria|Rep: Hydrogenase large subunit - Aquifex
aeolicus
Length = 633
Score = 31.1 bits (67), Expect = 6.3
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 120 PEQWANSRVGRRQAGALXCQLRRHXQVLWSRYL 218
PE+W + VGR A AL Q+ H + W + L
Sbjct: 472 PEKWLPTTVGRTIARALEAQMSAHTNLYWMKKL 504
>UniRef50_Q1QKG4 Cluster: Putative uncharacterized protein
precursor; n=3; Nitrobacter|Rep: Putative
uncharacterized protein precursor - Nitrobacter
hamburgensis (strain X14 / DSM 10229)
Length = 216
Score = 31.1 bits (67), Expect = 6.3
Identities = 24/103 (23%), Positives = 40/103 (38%)
Frame = +2
Query: 56 SGHSRYVLVKAWLLH*TI*GSAGAVGQLQGWXPASGCTXLSTQTAXSGAMVKVPITGNEN 235
+GH + + L G+ A G ++ ASG T A V +
Sbjct: 66 TGHKSHRRIPVGLARVMAEGAGRAAGVVRS---ASGATAHVASRATGAFQCLVSALDRQG 122
Query: 236 HRLSALGSVDLTNQMKLGAATAGLAYDNVNGHGATLTKTHIPG 364
+ + +G ++ AGLA D +N HG +T+ +PG
Sbjct: 123 YPIRFMGGWRAHGSVRASLHPAGLALD-INQHGRNITRPRMPG 164
>UniRef50_Q2CEK6 Cluster: Putative uncharacterized protein; n=1;
Oceanicola granulosus HTCC2516|Rep: Putative
uncharacterized protein - Oceanicola granulosus HTCC2516
Length = 154
Score = 30.7 bits (66), Expect = 8.3
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Frame = +2
Query: 254 GSVDLTNQMKLGAATAGLAYDNV---NGHGATLTKTHI 358
GS+DL+ +GAAT G D NGH AT+ +T I
Sbjct: 65 GSIDLSAYPVVGAATTGAIADVTSVGNGHSATIVQTGI 102
>UniRef50_Q8W2Y1 Cluster: Putative polyprotein protein; n=2; Oryza
sativa (japonica cultivar-group)|Rep: Putative
polyprotein protein - Oryza sativa subsp. japonica
(Rice)
Length = 742
Score = 30.7 bits (66), Expect = 8.3
Identities = 22/76 (28%), Positives = 33/76 (43%)
Frame = -2
Query: 294 AAPNFIWLVRSTEPRALSL*FSFPVIGTLTIAPEXAV*VDXLVHPLAGXQPWSWPTAPAD 115
A FIWL A++ + + +A AV +D L P+A +P + AP+
Sbjct: 460 ATDGFIWLAAVIRKTAVAAASVEGALRQMLVAAGVAVALDKLAAPVALDKPAANAAAPSP 519
Query: 114 PHIVQCSNQAFTSTYR 67
P I S +ST R
Sbjct: 520 PVIALASLAMISSTTR 535
>UniRef50_Q336X3 Cluster: Retrotransposon protein, putative, LINE
subclass; n=3; Oryza sativa|Rep: Retrotransposon
protein, putative, LINE subclass - Oryza sativa subsp.
japonica (Rice)
Length = 808
Score = 30.7 bits (66), Expect = 8.3
Identities = 22/76 (28%), Positives = 33/76 (43%)
Frame = -2
Query: 294 AAPNFIWLVRSTEPRALSL*FSFPVIGTLTIAPEXAV*VDXLVHPLAGXQPWSWPTAPAD 115
A FIWL A++ + + +A AV +D L P+A +P + AP+
Sbjct: 219 ATDGFIWLAAVIRKTAVAAASVEGALRQMLVAAGVAVALDKLAAPVALDKPAANAAAPSP 278
Query: 114 PHIVQCSNQAFTSTYR 67
P I S +ST R
Sbjct: 279 PVIALASLAMISSTTR 294
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 371,313,490
Number of Sequences: 1657284
Number of extensions: 6732831
Number of successful extensions: 15808
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 15381
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15808
length of database: 575,637,011
effective HSP length: 90
effective length of database: 426,481,451
effective search space used: 12794443530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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