Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0002_M04
         (531 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...   185   4e-48
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...   140   1e-34
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    81   1e-16
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe...    27   1.3  
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch...    25   5.3  
SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc...    25   7.0  
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    25   7.0  
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom...    25   7.0  
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch...    25   9.3  
SPAC25B8.13c |isp7||2-OG-Fe|Schizosaccharomyces pombe|chr 1|||Ma...    25   9.3  
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz...    25   9.3  

>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score =  185 bits (450), Expect = 4e-48
 Identities = 91/170 (53%), Positives = 116/170 (68%)
 Frame = +3

Query: 3   NVVHVKSLPNVKCRHHDVDCIIIREQTEGEYSALEHESVPGVVECLKIITAAKSERIAKF 182
           ++V +K++P  K RH +VD  IIRE TEGEYS LEH+SVPGVVE LKIIT  KS+RIA+F
Sbjct: 114 SLVLIKNIPGFKTRHDNVDFAIIRENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQF 173

Query: 183 AFDYAVKMGRKKVTAVHKANIMKLGDGLFLRSCEEMAKLYPRIQFEKMIVDNCTMQMVSN 362
           AFD+A++ GRK VT +HKANIMKL DGLF R+  ++A  Y  I  + +IVDN +MQ VS 
Sbjct: 174 AFDFALQNGRKSVTCIHKANIMKLADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSR 233

Query: 363 PNQFDVMVTPNLYGNIVDNXXXXXXXXXXXXXXXXXXXECAVFEQGARHI 512
           P QFDV+V PNLYG+I+ N                   + A+FE G RH+
Sbjct: 234 PQQFDVLVMPNLYGSILSNIGSALVGGPGVIPGANFGRDYALFEPGCRHV 283


>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  140 bits (339), Expect = 1e-34
 Identities = 65/141 (46%), Positives = 98/141 (69%), Gaps = 2/141 (1%)
 Frame = +3

Query: 3   NVVHVKSLPNVKCRHHDVDCIIIREQTEGEYSALEHESVPGVVECLKIITAAKSERIAKF 182
           NV    S+   K  + +V+ ++IRE TEGEYS +EHE +PGVV+ +K+IT A SER+ ++
Sbjct: 136 NVRPCVSITGYKTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRY 195

Query: 183 AFDYAVKMGRKKVTAVHKANIMKLGDGLFLRSCEEMAKLYPRIQFEKMIVDNCTMQMVSN 362
           AF YA + G+  +T VHKA IM++ DGLFL   +E+A  YP I+  + I+DN  +++V++
Sbjct: 196 AFQYARQTGKNNITVVHKATIMRMADGLFLECAKELAPEYPDIELREEILDNACLKIVTD 255

Query: 363 PNQFD--VMVTPNLYGNIVDN 419
           P  ++  VMV PNLYG+IV +
Sbjct: 256 PVPYNNTVMVMPNLYGDIVSD 276


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 81.0 bits (191), Expect = 1e-16
 Identities = 58/158 (36%), Positives = 82/158 (51%), Gaps = 19/158 (12%)
 Frame = +3

Query: 3   NVVHVKSLPNVKCRHHDVDCIIIREQTEGEYSALEH--ESVPG--VVECLKIITAAKSER 170
           NV  VKSL   K +   VD +I+RE TE  Y   E   ++ PG  V E ++ I+   S +
Sbjct: 105 NVRPVKSLDGAKGK--PVDLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTK 162

Query: 171 IAKFAFDYAVKMGR----------KK--VTAVHKANIMKLGDGLFLRSCEEMAKL---YP 305
           I K AF+ A    +          KK  VT +HK+N+M + DGLF  SC     L   Y 
Sbjct: 163 IGKMAFEIAKSRQKIRESGTYSIHKKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYA 222

Query: 306 RIQFEKMIVDNCTMQMVSNPNQFDVMVTPNLYGNIVDN 419
            I  ++ IVD+   ++   P  FDV+V PNLYG+I+ +
Sbjct: 223 SINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSD 260


>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 14/41 (34%), Positives = 19/41 (46%)
 Frame = +2

Query: 35  EVPASRCRLHHHQRTDRRRILRSGT*VCAWRGRVLEDYHRC 157
           EV  S C ++  Q      I  +G  V AW+G   E+Y  C
Sbjct: 72  EVTWSSCNIYSTQDHAAAAIAATGVPVFAWKGETEEEYLWC 112


>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
 Frame = +3

Query: 18  KSLPNVKC--RHHDVDCIIIREQTEGEYSALEHESV 119
           K++PNV    R H +  + + EQ  GE S+ EH ++
Sbjct: 636 KTVPNVAIDYRDHGIRWVTLGEQNFGEGSSREHAAL 671


>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
           L20|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score = 25.0 bits (52), Expect = 7.0
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 182 EFRDTFRFRSGDNLQALDHARHRLMFQS 99
           EFRDT R  + + + A   ARHR  F+S
Sbjct: 92  EFRDTTRVGAVEAMYADMAARHRARFRS 119


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
            Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 25.0 bits (52), Expect = 7.0
 Identities = 7/23 (30%), Positives = 14/23 (60%)
 Frame = -3

Query: 388  VTITSNWFGFDTICIVQLSTIIF 320
            + ++  W G +T+  V LS ++F
Sbjct: 1076 IAVSDKWLGAETVTPVSLSNVVF 1098


>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
           protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score = 25.0 bits (52), Expect = 7.0
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 182 EFRDTFRFRSGDNLQALDHARHRLMFQS 99
           EFRDT R  + + + A   ARHR  F+S
Sbjct: 92  EFRDTTRVGAVEAMYADMAARHRARFRS 119


>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 872

 Score = 24.6 bits (51), Expect = 9.3
 Identities = 14/51 (27%), Positives = 26/51 (50%)
 Frame = -2

Query: 506 PSALFEDGTLRAVGSAGDDTGPANKSAGEVVYYVAVQVGRDHHVELVRVRY 354
           P  LF D T+ ++ +  + T  A      + +++ + VGR  H E++ V Y
Sbjct: 704 PMNLFSDQTIGSITNTIEPTAAAKNVVQFLGFHMRILVGRADH-EILDVFY 753


>SPAC25B8.13c |isp7||2-OG-Fe|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 397

 Score = 24.6 bits (51), Expect = 9.3
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -2

Query: 209 AHLDRVIESEFRDTFRFRSGDNL 141
           AH D  I +EFR++F F   DNL
Sbjct: 171 AHGDESISNEFRESFYF-GNDNL 192


>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 676

 Score = 24.6 bits (51), Expect = 9.3
 Identities = 10/28 (35%), Positives = 18/28 (64%), Gaps = 3/28 (10%)
 Frame = -2

Query: 437 NKSAGEVVYYVAVQVGRDHH---VELVR 363
           NK   ++VY   +++GR HH   +EL++
Sbjct: 608 NKKVRQLVYDDLIRIGRSHHFANIELIQ 635


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,112,523
Number of Sequences: 5004
Number of extensions: 42088
Number of successful extensions: 124
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)