BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_L04
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.59
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 28 1.0
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 27 2.4
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 27 2.4
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 5.5
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 25 7.3
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 25 9.6
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 29.1 bits (62), Expect = 0.59
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -1
Query: 534 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 421
CI GF ++ + +RTR + PS +LS T V+ FL
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS-AFKTLSLTSVMVTSFL 361
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 28.3 bits (60), Expect = 1.0
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 534 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 421
CI GF ++ + +RTR + PS +LS + V+ FL
Sbjct: 325 CIAGFVVNELYTTRTRIIAPS-AFQTLSLSSVMVTSFL 361
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 27.1 bits (57), Expect = 2.4
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +2
Query: 29 MLPTDLEPALSFCTHLLYKSAGIQADTYKMVSLNENLD 142
M+ +D EP +S C LL + D + MVS E +
Sbjct: 1281 MVESDYEPDVSLCPELLSLAIDFPGDPFVMVSKMEKYE 1318
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 27.1 bits (57), Expect = 2.4
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -2
Query: 272 PIKDCTSVDLQCRHHLPLSR 213
P K+C + L+C +H+P SR
Sbjct: 46 PCKNCKAGKLECTYHMPSSR 65
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 616 DNVDQVYDAWDVKVNGAIYVWQ 551
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 25.4 bits (53), Expect = 7.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +2
Query: 281 QARTAFTNSALLLAEQYGFDGIDLSWQLPK 370
Q T F S+L +A G+D I L W L K
Sbjct: 531 QGVTEFVPSSLGVALAKGYDEIGLEWSLTK 560
>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
L32|Schizosaccharomyces pombe|chr 2|||Manual
Length = 103
Score = 25.0 bits (52), Expect = 9.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 485 ALVREMKQALNVKPNMQLVISVLPNVNCSIYFDVPSIINL 604
AL+R A++ K S LP+++ I+ PSI N+
Sbjct: 2 ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,616,197
Number of Sequences: 5004
Number of extensions: 52101
Number of successful extensions: 169
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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